马铃薯致病疫霉拮抗细菌研究进展

为了解决马铃薯致病疫霉侵染马铃薯导致其死亡,进而造成马铃薯单位种植产量低的问题。本文从拮抗菌入手,归纳分析细菌类拮抗菌中的主要拮抗菌中芽孢杆菌、假单胞菌和粘细菌对马铃薯致病疫霉的抑制作用。同时,在总结前人研究的基础上,笔者认为在拮抗菌应用方面,可以采用多对一的新型平板对峙筛菌模式来模拟复杂条件下拮抗菌的拮抗作用,以期得到稳定的拮抗菌;并指出应从定植能力和根际微生物影响方面对拮抗菌实际应用的可行性进行研究;此外,提出未来对拮抗菌抑制马铃薯致病疫霉的探究应从表型层面的研究深入到分子层面,以期能成功从源头找寻抑制马铃薯致病疫霉的答案。     

强化型EM菌剂对金针菇菌糠堆肥的影响

为提高EM菌剂在菌糠堆肥中的应用效果,加速腐熟进程,改善堆肥质量,采用纤维素酶与木聚糖酶高产菌株黑曲霉SNH-7、蛋白酶高产菌株枯草芽孢杆菌SNK-103与EM菌剂进行复配,研制强化型EM菌剂,并研究其对菌糠堆肥的影响。结果表明：与普通EM菌剂相比,接种强化型EM菌剂的处理堆肥过程中微生物代谢更加旺盛,温度、pH、EC值、总有机碳、可溶性有机碳、总氮、铵态氮、硝态氮、C/N、腐植酸和黄腐酸含量等理化指标的升高或降低幅度更大,腐熟进程加快;成品堆肥的GI提高,C/N降低,总氮、硝态氮、总腐植酸和游离腐植酸的含量升高,生物安全性更好、肥效更高。说明在EM菌剂中补充纤维素酶、木聚糖酶和蛋白酶高产菌株,可强化其对纤维素、木质素和蛋白质的降解能力,在无辅料、高C/N、低pH的不利条件下,添加该菌剂能加速堆肥进程,提高堆肥质量。     

Influence of temperature,humidity duration and growth stage on the infection and mycotoxin production by Fusarium langsethiae and Fusarium poae in oats

High occurrence of Fusarium poae (FP) and Fusarium langsethiae (FL) and their mycotoxins nivalenol (NIV) and T-2/HT-2 have been observed in Swiss oats. Early prediction of mycotoxin levels is important for farmers and the cereal industry to minimize the risk of contaminated food and feed. Therefore, climate chamber experiments were conducted to investigate the influence of different temperatures (10, 15, 20 °C) and durations (4, 8, 12 h) at 99% relative humidity (RH) on the infection of oats with FP and FL. In addition, to discover the most susceptible period of oats, artificial FL inoculations were conducted at different growth stages. Field experiments were performed to observe the dispersal of these fungal species within the field and to investigate the weather conditions that influence the dispersal. The climate chamber experiments revealed higher contamination with NIV and T-2/HT-2 in the 10 °C treatments and with a prolonged humidity duration of 12 h 99% RH. Inoculations of oat plants at early (DC 61) and mid (DC 65) anthesis, led to higher FL infection and T-2/HT-2 accumulation in the grains compared with treatments at earlier growth stages, which might be due to an increased susceptibility during anthesis. No indication for spore dispersal was observed in the field experiments. The results obtained, together with the cropping factors that influence infection and mycotoxin production, could be used as a first step in developing forecasting models to predict the contamination of oats with the mycotoxins NIV and T-2/HT-2.     

叶面滞尘量对大叶黄杨光谱特征影响研究

  目的  叶面滞尘会影响植被光谱特征，削弱植被指数对植被的响应能力，影响反演评估的准确性。为探究叶面滞尘量对植被光谱响应特征及预测模型的影响，本文以北京市常见常绿绿化树种大叶黄杨为研究对象展开研究。  方法  从封闭区域、半封闭区域、开放区域，采集叶片样本，并收集环境灰尘。通过室内控制试验，利用ASD FildSpec Handheld光谱仪测量不同滞尘量叶片的高光谱数据，选取5个特征波段，通过光谱角的方法研究了叶面滞尘量对叶片光谱特征的影响，以及滞尘量对叶面滞尘量预测模型的精度和稳定性影响。  结果  随着叶面滞尘量的增加，植被光谱曲线特征逐渐减弱，灰尘的特征逐渐增强，但光谱曲线的总体变化趋势基本一致。当叶面滞尘量 > 120 g/m2时，光谱曲线的基本表现为灰尘的光谱特征。当叶面滞尘量较少时，预测模型的模拟精度相对较高，随着滞尘量的增加，所有模拟预测模型的决定系数均减小；当叶面滞尘量 > 120 g/m2时，预测模型对叶面滞尘量的模拟预测能力将更差，并且均方根误差（RMSE）随着叶片单位面积滞尘量的增加而增大，模拟预测模型的稳定性及预测精度逐渐降低。光谱角对滞尘叶片350 ~ 1 770 nm波段区间的光谱变化十分敏感，利用叶片光谱角检测滞尘程度不需要分区域讨论，只需与阈值做简单的比较，方法简便易行。  结论  本研究通过室内控制试验，研究叶面滞尘量对植被光谱响应特征，可为建立滞尘植被光谱反射物理模型提供参考与借鉴。      

Vole abundance and reindeer carcasses determine breeding activity of Arctic foxes in low Arctic Yamal,Russia

Background

High latitude ecosystems are at present changing rapidly under the influence of climate warming, and specialized Arctic species at the southern margin of the Arctic may be particularly affected. The Arctic fox (Vulpes lagopus), a small mammalian predator endemic to northern tundra areas, is able to exploit different resources in the context of varying tundra ecosystems. Although generally widespread, it is critically endangered in subarctic Fennoscandia, where a fading out of the characteristic lemming cycles and competition with abundant red foxes have been identified as main threats. We studied an Arctic fox population at the Erkuta Tundra Monitoring site in low Arctic Yamal (Russia) during 10 years in order to determine which resources support the breeding activity in this population. In the study area, lemmings have been rare during the last 15 years and red foxes are nearly absent, creating an interesting contrast to the situation in Fennoscandia.

Results

Arctic fox was breeding in nine of the 10 years of the study. The number of active dens was on average 2.6 (range 0–6) per 100 km² and increased with small rodent abundance. It was also higher after winters with many reindeer carcasses, which occurred when mortality was unusually high due to icy pastures following rain-on-snow events. Average litter size was 5.2 (SD = 2.1). Scat dissection suggested that small rodents (mostly Microtus spp.) were the most important prey category. Prey remains observed at dens show that birds, notably waterfowl, were also an important resource in summer.

Conclusions

The Arctic fox in southern Yamal, which is part of a species-rich low Arctic food web, seems at present able to cope with a state shift of the small rodent community from high amplitude cyclicity with lemming dominated peaks, to a vole community with low amplitude fluctuations. The estimated breeding parameters characterized the population as intermediate between the lemming fox and the coastal fox ecotype. Only continued ecosystem-based monitoring will reveal their fate in a changing tundra ecosystem.

     

Impact of grassland contract policy on soil organic carbon losses from alpine grassland on the Qinghai–Tibetan Plateau

Carbon storage in the soils on the Qinghai–Tibetan Plateau plays a very important role in the global carbon budget. In the 1990s, a policy of contracting collective grasslands to smaller units was implemented, resulting in a change from the traditional collective grassland management to two new management patterns: a multi‐household management pattern (MMP: grassland shared by several households without enclosures) and a single‐household management pattern (SMP: grassland enclosed and used by only one household). In 2016, 50 MMP and 54 SMP winter pastures on the Qinghai–Tibetan Plateau were sampled to assess the differences in soil organic carbon (SOC) between the two management patterns. Results showed that average SOC was significantly greater under MMP than under SMP, with an estimated 0.41 Mg C/ha/yr lost due to SMP following the new grassland contract. Based on the government's grassland policy, four grassland utilization scenarios were developed for both summer and winter pastures. We found that if the grassland were managed under SMP, likely C losses ranged between 0.31 × 10⁷ and 6.15 × 10⁷ Mg C/yr across the Qinghai–Tibetan Plateau relative to MMP, which more closely resembles pre‐1990s grassland management. Previous estimates of C losses have only considered land use change (with cover change) and ignored the impacts driven by land management pattern changes (without cover change). The new data suggest that C losses from the Qinghai–Tibetan Plateau are greater than previously estimated, and therefore that the grassland contract policy should be reviewed and SMP households should be encouraged to reunite into the MMP. These findings have potential implications for land management strategies not only on the Qinghai–Tibetan Plateau but also other grazing regions globally where such practices may exist.     

Utilizing a DUALEM‐421 and inversion modelling to map baseline soil salinity along toposequences in the Hunter Valley Wine district

In the oldest commercial wine district of Australia, the Hunter Valley, there is the threat of soil salinization because marine sediments underlie the area. To understand the risk requires information about the spatial distribution of soil properties. Electromagnetic (EM) induction instruments have been used to identify and map the spatial variation of average soil salinity to a certain depth. However, soils vary with depth dependent on soil forming factors. We collected data from a single‐frequency and multiple‐coil DUALEM‐421 along a toposequence. We inverted this data using EM4Soil software and evaluated the resultant 2‐dimensional model of true electrical conductivity (σ – mS/m) with depth against electrical conductivity of saturated soil pastes (EC_p – dS/m). Using a fitted linear regression (LR) model calibration approach and by varying the forward model (cumulative function‐CF and full solution‐FS), inversion algorithm (S1 and S2), damping factor (λ) and number of arrays, we determined a suitable electromagnetic conductivity image (EMCI), which was optimal (R² = 0.82) when using the full solution, S2, λ = 3.6 and all six coil arrays. We conducted an uncertainty analysis of the LR model used to estimate the electrical conductivity of the saturated soil‐paste extract (EC_e – dS/m). Our interpretation based on estimates of EC_e suggests the approach can identify differences in salinity, how these vary with parent material and how topography influences salt distribution. The results provide information leading to insights into how soil forming factors and agricultural practices influence salinity down a toposequence and how this can guide soil management practices.     

<Emphasis Type="Italic">Lupinus albus</Emphasis> Conglutin Gamma Modifies the Gene Expressions of Enzymes Involved in Glucose Hepatic Production <Emphasis Type="Italic">In Vivo</Emphasis>

Lupinus albus seeds contain conglutin gamma (Cγ) protein, which exerts a hypoglycemic effect and positively modifies proteins involved in glucose homeostasis. Cγ could potentially be used to manage patients with impaired glucose metabolism, but there remains a need to evaluate its effects on hepatic glucose production. The present study aimed to analyze G6pc, Fbp1, and Pck1 gene expressions in two experimental animal models of impaired glucose metabolism. We also evaluated hepatic and renal tissue integrity following Cγ treatment. To generate an insulin resistance model, male Wistar rats were provided 30% sucrose solution ad libitum for 20 weeks. To generate a type 2 diabetes model (STZ), five-day-old rats were intraperitoneally injected with streptozotocin (150 mg/kg). Each animal model was randomized into three subgroups that received the following oral treatments daily for one week: 0.9% w/v NaCl (vehicle; IR-Ctrl and STZ-Ctrl); metformin 300 mg/kg (IR-Met and STZ-Met); and Cγ 150 mg/kg (IR-Cγ and STZ-Cγ). Biochemical parameters were assessed pre- and post-treatment using colorimetric or enzymatic methods. We also performed histological analysis of hepatic and renal tissue. G6pc, Fbp1, and Pck1 gene expressions were quantified using real-time PCR. No histological changes were observed in any group. Post-treatment G6pc gene expression was decreased in the IR-Cγ and STZ-Cγ groups. Post-treatment Fbp1 and Pck1 gene expressions were reduced in the IR-Cγ group but increased in STZ-Cγ animals. Overall, these findings suggest that Cγ is involved in reducing hepatic glucose production, mainly through G6pc inhibition in impaired glucose metabolism disorders.     

Hybrid breeding for biomass yield in winter triticale: II. Combining ability and hybrid prediction

Accurate hybrid prediction and knowledge about the relative contribution of general (GCA) and specific combining ability (SCA) are of utmost importance for efficient hybrid breeding. We therefore evaluated 91 triticale single-cross hybrids in field trials at seven environments for plant height, heading time, fresh biomass, dry matter content and dry biomass. Fresh and dry biomass showed the highest proportion (23%) of variance due to SCA. Prediction accuracies based on GCA were slightly higher than based on mid-parent values. Utilizing parental kinship information yielded the highest prediction accuracies when both parental lines have been tested in other hybrid combinations, but still moderate-to-low prediction accuracies for two untested parents. Thus, hybrid prediction for biomass traits in triticale is currently promising based on mid-parent values as emphasized by our simulation study, but can be expected to shift to GCA-based prediction with an increasing importance of GCA due to selection in hybrid breeding. Moreover, the performance of potential hybrids between newly developed lines can be predicted with moderate accuracy using genomic relationship information.