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European Journal of Forest Research - Rare domestic tree species are increasingly being viewed as promising alternatives and additions to current main tree species in forests facing climate change....  相似文献   
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Plasmin is a protease in milk, which concentration is increased during mastitis. The aim of the study was design and characterize short, tailor made fluorogenic substrates for plasmin to see differences in plasmin activity in healthy milk compared to mastitic milk. According to the specificity matrix of plasmin, containing 125 currently known plasmin substrates, six novel plasmin specific substrates were designed and characterized. Eight other substrates were insensitive for plasmin, which was in line with our expectations. The plasmin-sensitive substrates showed no cross-reactivity with proteases from mastitogenic bacteria in vitro. A proof of concept to distinguish healthy milk from mastitic milk is described. Potentially, these substrates present a rapid alternative to the currently established methodologies to detect mastitis in milk.  相似文献   
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Abstract

A group of spring wheat cultivars originating from Sweden, Finland, Norway, Germany, and the Netherlands was analysed for powdery mildew resistance. Using functional molecular markers, two alleles of the major resistance gene Pm3 were detected among the cultivars under the study. One of the alleles, Pm3d, was detected in the resistant cultivars ‘Vinjett’, ‘SW Estrad’, and ‘Zebra’, and in ‘Tjalve’, a cultivar of earlier release susceptible to the local population of powdery mildew. The second allele of Pm3 detected in the analysed group of cultivars was the allele Pm3f, rarely used in Europe. It was identified in the resistant cultivars ‘Satu’, ‘Helle’, and in the moderatively resistant cultivar ‘Mahti’. Pm3f was found to be effective against the local population of powdery mildew in Estonia, while Pm3d provided no protective effect. Besides the Pm3d allele on chromosome 1A, monosomic analysis of the cultivar ‘Vinjett’,which is almost immune to powdery mildew, identified two additional loci on chromosomes 5D and 7D, respectively, presumably responsible for the high resistance in this cultivar. In contrast to the earlier cultivars, six recently released cultivars (‘Vinjett’, ‘SW Estrad’, ‘Zebra’, ‘Satu’, ‘Helle’, ‘Meri’) demonstrated a high resistance to the powdery mildew fungus Blumeria graminis DC. f. sp. tritici both in the field and seedling tests, showing that the genetic basis of powdery mildew resistance in Nordic spring wheat has been improved noticeably in the last ten years.  相似文献   
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Ethnic entrepreneurship has become a popular concept in a modern multi–cultural society. This paper seeks to offer an overview of the potential of ethnic entrepreneurship for solving inter alia the structural unemployment problems of ethnic groups in cities. There is a general lack of empirical evidence on this issue in the current literature. The present paper aims to fill this gap by addressing in particular the critical success conditions for ethnic entrepreneurs. The focus of the research is on variations in success across three ethnic groups in the Amsterdam area. By means of structured personal interviews with many ethnic entrepreneurs, a systematic qualitative data base was created. The paper sets out to identify empirically the driving forces for business success, such as education or the role of informal networks. The explanatory framework deployed for the identification of these qualitative success factors for distinct ethnic groups is based on a particular, recently developed artificial intelligence method, viz. rough set analysis. This multidimensional classification approach appears to be able to identify various important factors for the motivation and performance of ethnic enterprises. Two major findings emerge from this investigation: (i) performance conditions vary across ethnic groups, and (ii) informal networks are crucial for business success.  相似文献   
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Ammonium (NH ) nutrition causes retardation of growth in many plant species. In Arabidopsis grown with NH as the sole N source, growth retardation occurs already at early stages before photosynthesis has come to its full power. In order to describe the peculiarities of these retarded plants, they were compared with nitrate (NO )‐grown plants of the same age of 15 d. Photosynthetic activity as measured by CO2 uptake per unit chlorophyll is half as high in NH ‐grown seedlings as in NO ‐grown ones. This finding is confirmed by the analysis of chlorophyll fluorescence. Chloroplasts of NO ‐grown, but not of NH ‐grown, seedlings show starch deposits after 5 h of illumination with 40 μmol m–2 s–1. Gene‐expression analysis based on cDNA microarray and on Northern blots provide a clue about the biochemical background. After the first 2 weeks of growth, it seems that NO ‐grown seedlings subsist mainly on normal photosynthesis, whereas NH ‐grown seedlings still use lipids from the seeds stored in oleosomes. Corresponding to this observation, the mRNAs for enzymes of β‐oxidation are more strongly expressed in NH ‐grown seedlings. Different carbohydrate sources for sucrose synthesis are indicated by different gene expression. Higher gene expression of fructose bisphosphate aldolase (cytosolic isoform) in NO ‐grown seedlings indicates the dependence on photosynthesis, whereas a higher gene expression of PEP carboxykinase in NH ‐grown seedlings points to a prominent role of β‐oxidation of storage lipids still present.  相似文献   
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We analyzed the distribution of branched tetraether membrane lipids derived from soil bacteria in a marine sediment record that was recovered close to the Congo River outflow, and the results enabled us to reconstruct large-scale continental temperature changes in tropical Africa that span the past 25,000 years. Tropical African temperatures gradually increased from approximately 21 degrees to 25 degrees C over the last deglaciation, which is a larger warming than estimated for the tropical Atlantic Ocean. A direct comparison with sea-surface temperature estimates from the same core revealed that the land-sea temperature difference was, through the thermal pressure gradient, an important control on central African precipitation patterns.  相似文献   
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