A simplified roller bottle platform for the production of a new generation VLPs rabies vaccine for veterinary applications

Rabies is a neglected disease with an estimated annual mortality of 55,000 human deaths, affecting mainly low-income countries. Over 95% of these cases result from virus transmission through the bite of infected dogs and for this reason there is a real need for a cheap and effective rabies veterinary vaccine to be used in mass vaccination campaigns. In this work, we describe the establishment of a simple platform for the production of a virus-like particles based rabies vaccine using mammalian cells and roller bottles as culture system. Adherent cells were cultured during more than 15 days and VLPs were continuously produced and secreted to the culture supernatant. Immunogenicity and protective efficacy of VLPs were tested through rabies virus neutralizing antibody test and NIH potency test. These viral particles induced high titer of long lasting neutralizing antibodies and protected mice against active virus challenge. Therefore, this development represents a promising platform for the production of a new generation and virus-free rabies vaccine candidate for veterinary applications.     

h指数及其衍生指标评估兽用疫苗领域单篇专利的适用性探索

随着禽流感、非洲猪瘟等疾病的出现,兽用疫苗的开发研制成为了动物防疫体系建设的重要一环,同时兽用疫苗领域国际竞争日益激烈,直接影响到我国相关企业的生存和发展,因此国家必须加强对兽用疫苗领域专利质量和专利保护的重视.本文以计量学中的h指数及其衍生指标为对象,研究其与传统专利价值评估指标的关系,通过相关性分析来筛选评估兽用疫苗领域高价值专利的指标.结果表明:h指数、g指数和hg指数这三个指标,与传统专利价值评估指标相关性强,且计算简便,相对于传统指标,能够快速准确地对兽用疫苗领域专利价值进行预估.     

《兽医临床诊断学》教学改革效果分析

对近3年<兽医临床诊断学>教学改革的效果进行了分析,具体包括教学方法改革效果、实践教学改革效果、考核方式改革效果以及其他方面的改革效果,旨在为兽医临床诊断学及其他课程教学提供有益参考.     

非洲猪瘟疫情背景下兽医公共卫生学课程的教学策略

兽医公共卫生学是利用一切与人类和动物健康问题有关的理论知识、实践活动和物资资源,为人类健康事业服务的一门综合性应用学科,在动物性食品安全、动物疫病及人兽共患病方面发挥着重要的防线作用。针对当前我国发生的非洲猪瘟疫情,在阐述兽医公共卫生学重要性的基础上,分析了兽医公共卫生学课程的教学策略,以期打破对兽医专业认识的局限性,强化理解兽医公共卫生的意义,提升兽医公共卫生学的课程价值,并为优化教学内容、提高教学质量提供指导。