鸡传染性法氏囊病间接ELISA诊断试剂盒的研制及初步应用

采用vero细胞增殖的IBDV抗原建立了间接ELISA，用于定量检测IBDV抗体。对该方法进行了标准化研究，确立了该方法的最佳工作条件。在此基础上研制了相应的诊断试剂盒，并对盒内各组分的性状、保存、质量控制以及诊断试剂盒的特异性、敏感性、可重复性、符合率、与国外同类产品的比较、保存期等进行了全面、详细的研究。结果表明，研制的试剂盒在-20℃保存至6个月时各项性能都很好。该试剂盒与进口试刺盒对同样血清样品检测，符合率为86．7％。间接ELISA试剂盒与琼扩试验的符合率为92，5％。该试刺盒可对大量血清样品进行检测，操作简单方便、结果特异性强、敏感性高、快速，更适合于大型鸡场IBD免疫抗体水平的监测及现地疫病诊断等需要。     

芪蓝抗毒饮治疗鸡传染性法氏囊病的剂量研究

比较了芪蓝抗毒饮三种剂量和中剂量三种给药次数对雏鸡实验性传染性法氏囊病的疗效。结果表明，中剂量和发病后该剂量每天给药1次、连续4天的疗效最好。     

Effect of the Immunity Efficacy of IBD Immune Complex Vaccine on ND La Sota Vaccine

1-day-old SPF chickens and commercial Jingfen chickens were vaccinated with IBD immune complex(IC) vaccine, NDV La Sota vaccine were inoculated simultaneously every one week and every two weeks.NDV La Sota immunization alone was as the control group.At the 2nd, 3rd, 4th and 5th week post inoculation, blood samples were taken and the ND HI antibody were tested.Experimental chickens were challenged with high virulent NDV at the 5th week post inoculation, the protective rate of each group was calculated.The results showed the ND HI antibody were not significant different in the combined immunization of IBD IC vaccine priming and NDV La Sota vaccine boost and NDV La Sota vaccine alone immunization (P>0.05).The results indicated that IBD IC vaccine has no immunosuppression on NDV La Sota vaccine in SPF chickens and commercial Jingfen chickens.     

2003～2005年河南省鸡传染性法氏囊病流行病学调查

对2003～2005年间河南省15个地区鸡群传染性法氏囊病的流行情况进行了调查。发现传染性法氏囊病的发病有全年化趋势,但每年仍以6～9月份为发病高峰期;发病鸡的日龄范围扩大;病变以法氏囊呈胶胨样水肿和肾脏尿酸盐沉积为主要特征,二者出现率分别占67.2%和73.11%,其它病变多不典型;发病鸡群中免疫鸡群占78.17%;二次发病鸡群约占16.98%;宿主和易感群体的范围扩大。对出现上述情况进行了初步的分析并提出相应的措施。     

CD2配体在IBD和ND疫苗免疫应答中的佐剂效应研究

分别以猪源ＣＤ２配体协同新城疫（ＮＤ）Ⅳ疫苗免疫１８日龄鸡和绵羊源ＣＤ２配体协同法氏囊（ＩＢＤ）中等毒力疫苗免疫２５日龄鸡，采用β－微量法和琼脂扩散法测ＮＤ抗体及ＩＢＤ抗体水平。结果表明，两种ＣＤ２配体均能促进鸡体内特异性抗体的提前产生，并且试验组鸡抗体效价显著高于对照。     

Diagnostic evaluation and short-term outcome as indicators of long-term prognosis in horses with findings suggestive of inflammatory bowel disease treated with corticosteroids and anthelmintics

Background

Recurrent colic and unexplained weight loss despite good appetite and adequate feeding and management practices are common conditions in the horse. However, little information has been published on the systematic diagnostic evaluation, response to treatment, prognostic factors or outcome of either presentation. The aims of this study were to 1) identify possible prognostic indicators and 2) report the short- and long-term response to treatment with corticosteroid therapy of a variety of horses with a presumptive diagnosis of inflammatory bowel disease (IBD).Thirty-six horses with a history of recurrent colic and/or unexplained weight loss were screened with a detailed clinical, clinicopathological and diagnostic imaging examination. Twenty horses were subsequently selected that had findings consistent with inflammatory bowel disease based on the fulfilment of one or more of the following additional inclusion criteria: hypoproteinaemia, hypoalbuminaemia, malabsorption, an increased intestinal wall thickness on ultrasonographic examination or histopathological changes in rectal biopsy. These 20 horses were treated with a standardized larvicidal anthelmintic regime and a minimum of three weeks of corticosteroid therapy.

Results

The initial response to treatment was good in 75% (15/20) of horses, with a 3-year survival rate of 65% (13/20). The overall 3-year survival in horses that responded to initial treatment (12/15) was significantly higher (P = 0.031) than in those that did not respond to initial treatment (1/5). The peak xylose concentration was significantly (P = 0.048) higher in survivors (1.36 ± 0.44 mmol/L) than non-survivors (0.94 ± 0.36 mmol/L).

Conclusions

The overall prognosis for long-term survival in horses with a presumptive diagnosis of IBD appears to be fair to moderate, and the initial response to anthelmintic and corticosteroid therapy could be a useful prognostic indicator. The findings of the present study suggest that a low peak xylose concentration in absorption testing is associated with a less favourable prognosis, supporting the use of this test.     

鸡传染性法氏囊病快速诊断试纸的研制

本文简述用胶体金免疫层析技术,构建鸡传染性法氏囊病快速诊断试纸。试纸主要由测试端、显色区和手柄端3部分构成,能在很短的时间内完成对鸡传染性法氏囊病的诊断,且无需任何仪器设备,具有简便、快速、敏感、特异的特点。     

硒增强雏鸡对IBD抵抗力机制的细胞免疫研究

为了解硒对雏鸡传染性法氏囊病（ＩＢＤ）抵抗大的影响及其细胞免疫机制，自１日龄起给雏鸡分别饲喂基础（含硒０．０８６ｍｇ／ｋｇ）、补硒０．３ｍｇ／ｋｇ和０．６ｍｇ／ｋｇ的不同日粮，于４９日龄采用微量全血培养￣３Ｈ－ＴｄＲ掺入法测定其中自然发生ＩＢＤ后第１０天和正常对照雏鸡的Ｔ淋巴细胞转化率，并计算ＩＢＤ鸡死亡率。结果，两补硒组ＩＢＤ雏鸡死亡率显著低于基础日粮组ＩＢＤ雏鸡（Ｐ＜０．０１）；ＩＢＤ明显抑制雏鸡Ｔ淋巴细胞转化率（Ｐ＜０．０５；Ｐ＜０．０１）；补硒的正常对照雏鸡和ＩＢＤ雏鸡Ｔ淋巴细胞转化率分别高于各自基础日粮雏鸡（Ｐ＜０．０５；Ｐ＜０．０１）。结果显示补硒可提高雏鸡细胞免疫功能，从而增强雏鸡对ＩＢＤ抵抗力，降低雏鸡死亡率。     

MB43活疫苗免疫效果及对鸡法氏囊组织操作的研究

本试验对鸡传染性法氏囊病（IBD）MB43活疫苗免疫后鸡的囊体比、新城疫血凝抑制（ND-HI）抗体效价及IBD AGP效价进行检测。结果表明，含有IBD高母抗试验鸡在17日龄免疫MB43活疫苗后，囊组织有不同程度的损伤；对正常免疫的ND-HI抗体效价无影响；试验鸡16天后IBD AGP阳性率为100%；21天攻毒保护率100%。     

鸡传染性法氏囊病超强毒901株的分离和鉴定

从暴发鸡传染性法氏囊病死亡率６３．５１％的病死鸡中，分离出一株ＩＢＤ病毒９０１株，经检测，９０１株是一株ＩＢＤＶ超强毒株，可使３１日龄健康雏鸡７１．４％死亡，１０日龄ＳＰＦ鸡胚和３０日龄ＳＰＦ雏鸡１００％死亡。死亡鸡再现了自然暴发ＩＢＤ的典型病变。