用免疫荧光、酶标技术检测赭曲霉毒素A的研究

该项研究采用本课题组提纯的赭曲霉毒素A(Ochratoxin A·简称OA)和研制的兔抗OA抗体等,进行间接荧光法和ELISA间接法检测OA的试验。通过对不同浓度的OA纯品和含毒饲料浸提液及中毒死亡鸡内脏的间接荧光染色,均能检出分布均匀、大小有别的黄绿色荧光亮点;用OA为被检抗原和兔抗OA抗体与羊抗兔IgG-HRP进行ELISA间接法试验,对含不同浓度OA的试验孔和对照孔的检测,结果均正确稳定。研究结果证明,以上两种检测方法检测OA均具有灵敏、特异、快速等优点。     

多肽生长因子生理功能的研究进展

多肽生长因子作为动物体细胞分泌的活性物质，具有重要的生理功能，其抗体对肿瘤也具有良好的抑制效果。本文对七种多肽生长因子在生理功能方面的研究进展做一综述。     

佐剂对鸡补体水平影响的研究

６０只未免疫的伊莎褐雄鸡雏分成４组，于２４日龄开始用试管法以５０％溶血度为标准样检测补体。３１日时Ａ，Ｂ，Ｃ三组分别皮下注射司盘－８０＋白油，氢氧化铝胶和ＢＣＧ，Ｄ组对照；４６日龄时再次注射。从３１日龄至５６日龄每隔５天检测一次。结果表明，佐剂使鸡的补体水平普遍升高。于每次注射后第５天达到高峰，第１０天左右接近正常。其中ＢＣＧ影响现大，并于５１日龄时天对照组异显著（０．０１＜Ｐ＜０．０５）．     

稻瘟菌诱导性稻叶脂氧合酶的进一步纯化及抗体制备

 采用DEAE-Toyopearl离子交换、Buty l-Toyopear l疏水层析、CM-Toyopearl离子交换、Sephacryl S100凝胶过滤和FPLCMono Q等步骤,从非亲和性稻瘟菌侵染稻叶中纯化了两种诱导性脂氧合酶,即CM-Loxl和CM-Lox2。SDS-PAGE检测结果表明:CM-Lox1和CM-Lox2为单链多肽,它们的分子量分别为98 kd和102 kd。利用微量免疫法制备了CM-Lox1和CM-Lox2的抗体,制备的抗体效价达到1:1000倍,能检测0.5~2.0 ng的抗原。免疫印迹杂交证明CM-Lox1和CM-Lox2之间存在密切的血清学关系。此外,Anti-CM-Lox1和Anti-CM-Lox2与水稻中另一稻瘟菌诱导性脂氧合酶RLL以及发芽种子出现的RL-2也存在交叉反应。     

酶联免疫吸附法测定畜禽组织中恩诺沙星的残留量

利用抗原抗体反应的高度特异性和酶的高效催化作用相结合,测定畜禽组织中恩诺沙星的残留量。结果表明,该检测方法简便、快速、灵敏。     

部分地区猪细环病毒1型感染的流行情况调查

猪细环病毒1型是近年来发现的又一种环状DNA病毒,可能存在潜在致病作用。为了初步了解其流行情况,用PCR技术和ELISA技术对来自京津冀、华东与华南地区578头保育猪血清样品进行了检测。结果显示,猪细环病毒1型的核酸与抗体的猪场阳性率均为100%;核酸阳性率为73.88%,抗体阳性率为78.72%,二者之间无显著差异（P>0.05）。进一步分析发现,猪细环病毒1型的核酸阳性率和抗体阳性率都超过50%的猪场达到86.67%,都超过80%的占53.33%。调查结果表明,猪细环病毒1型感染在我国部分地区普遍流行,其在多数猪场的感染率很高。     

黄瓜和葡萄霜霉病菌对不同内吸杀菌剂的交互抗药性

为了确定甲霜胺、乙磷铝与恶唑烷酮、霜脲氰、安克和硫代磷酰胺酯之间的交抗型,在叶盘上共测试了黄瓜霜霉(PC)和葡萄霜霉(PV)的27个分离系对6种内吸杀菌剂的抗药水平,结果证实了在两种目标真菌上甲霜胺和乙磷铝之间的非交抗关系,甲霜胺和恶唑烷酮之间的正交互抗药性,在PC上甲霜胺和霜脲氰之间的非交抗关系。试验还表明在PC上甲霜胺和安克之间以及乙磷铝和霜脲氰之间的非交抗关系,在PV上甲霜胺和乙磷铝与硫代磷酰胺酯之间的非交抗关系。此外,试验发现了霜脲氰和安克在叶盘上对抗甲霜胺分离系具有很高的杀菌活性。     

兔病毒性出血症首免时间的研究

针对生产上出现的从断奶育成这一年龄段多发兔病毒性出血症的实际情况，采用快速、敏感的Dot-ELISA方法，通过测定不同免疫状态下的母兔所产兔的母源抗体动态变化规律，并进一步测定不同抗体效价的成年兔攻毒后是否得到保护的结果，再结合生产实际，得出兔病毒性出血症首免时间为30日龄，且剂量加倍。     

Isotype-specific antibodies in horses and dogs with immune-mediated hemolytic anemia

Classes of antibody bound to erythrocytes were determined using direct immunofluorescence (DIF) flow cytometry in 3 horses and 12 dogs with immune-mediated hemolytic anemia (IMHA). Background levels of antibody binding were determined in samples from 12 horses and 12 dogs that were free of clinical disease. The range of nonspecific binding of a fluorescein isothiocyanate (FITC)-conjugated goat anti-equine immunoglobulin G (IgG) was 19.9–36.7%, but was eliminated by the use of the F(ab)₂ fragment of FITC-conjugated goat anti-equine IgG. Background binding by other class-specific antibodies to equine and canine erythrocytes was negligible. The DIF results were compared to the direct antiglobulin (Coombs) test in 5 horses and 20 dogs with anemia. The former assay was more sensitive in dogs with IMHA than was the Coombs' test (100% versus 58%). In contrast, the Coombs' test had better specificity than the DIF assay (100% versus 87.5%, respectively). Using clinical parameters or response to therapy as the comparison, the positive and negative predictive values for the DIF test were 92% and 100% compared to the values of the Coombs' test of 100% and 62%. The DIF assay detected low levels of cells bound with antibody (<30%) in 5 dogs that were Coombs' test-negative. For both species, performance of the DIF test was independent of the prozone effect. Five dogs with IMHA had IgG and IgM on erythrocytes, 5 had IgG, and 2 had IgM. Three horses had surface-bound IgG, including a horse with suspected penicillin-induced IMHA, a foal with neonatal isoerythrolysis, and a foal with clostridial septicemia. The DIF method was valuable in monitoring the response to therapy in the foal with neonatal isoerythrolysis.     

图书馆业务外包的核心问题——保证服务质量和水平

在图书馆业务外包环境下,如何保证服务质量这一关键性问题作出了一些探讨。