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排序方式: 共有109条查询结果,搜索用时 15 毫秒
1.
Yang Zi Chi Ma Huimin Li Suting Shen Yingchun Liu Ming Li Feng Gao 《Animal Science Journal》2021,92(1):e13613
This study investigated the effects of intrauterine growth restriction during late pregnancy on the ovine fetal renal function and renal antioxidant capacity. Eighteen ewes pregnant were randomly divided into control group (CG, ad libitum, 0.67 MJ ME·BW−0.75·day−1, n = 6), restricted group 1 (RG1, 0.18 MJ ME·BW−0.75·day−1, n = 6), and restricted group 2 (RG2, 0.33 MJ ME·BW−0.75·day−1, n = 6). At 140 days, the fetal blood, allantoic fluid and kidney tissue were collected to determinate fetal renal function and renal antioxidant capacity. The results showed that the fetal weight, kidney weight, superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), aquaporin-2 (AQP-2) and aquaporin-3 (AQP-3), and total antioxidant capacity (T-AOC) in RG1 group were decreased compared with the CG (P < 0.05), but the contents of β2-Microglobulin (β 2-MG), cystatin C (Cys-C), filtered sodium excretion fraction (FENa), malondialdehyde (MDA), and hydroxyl radical (OH) in RG1 group were increased (P < 0.05). The impaired ovine fetal renal growth, antioxidant imbalance and dysfunction of glomerulus ultrafiltration, and the renal tubules reabsorption were induced by maternal malnutrition during late pregnancy. 相似文献
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该研究应用膜孔径为100 nm的陶瓷膜错流超滤中试系统(MEMBRALOX?,Pall)对果胶酶酶解后的鲜榨苹果原汁进行了过滤澄清与除菌效果研究。结果表明,100 nm陶瓷膜错流超滤的最佳操作条件为:跨膜压力为0.85×105 Pa,温度为50℃,膜面流速为5 m/s;以0.5%次氯酸钠和4% NaOH溶液作为清洗剂,膜通量的恢复率可以达到98%。过滤后果汁中的悬浮颗粒和引起浊度升高的大分子胶体物质大部分被滤除,而果汁的各项质量指标如pH值、总酸、糖度等却未发生 相似文献
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Limited effects of land use on soil dissolved organic matter chemistry as assessed by excitation–emission fluorescence spectroscopy and molecular weight fractionation 下载免费PDF全文
P. Roberts J. M. Gibbons P. W. Hill D. L. Jones M. Farrell 《Soil Use and Management》2016,32(4):662-665
Dissolved organic matter (DOM) in soil solution represents a complex mixture of organic molecules and plays a central role in carbon and nitrogen cycling in plant–microbial–soil systems. We tested whether excitation–emission matrix (EEM) fluorescence spectroscopy can be used to characterize DOM and support previous findings that the majority of DOM is of high molecular weight (MW). EEM fluorescence spectroscopy was used in conjunction with MW fractionation to characterize DOM in soil solution from a grassland soil land management gradient in North Wales, UK. Data analysis suggested that three distinct fluorescence components could be separated and identified from the EEM data. These components were identified as being of humic‐like or fulvic‐like origin. Contrary to expectations, the majority of the fluorescence signal occurred in the small MW (<1 kDa) fraction, although differences between soils from the differently managed grasslands were more apparent in larger MW fractions. We conclude that following further characterization of the chemical composition of the fluorophores, EEM has potential as a sensitive technique for characterizing the small MW phenolic fraction of DOM in soils. 相似文献
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南极磷虾肽制备工艺优化及体外抗氧化研究 总被引:1,自引:1,他引:0
本文研究了3种不同的蛋白酶(木瓜蛋白酶、碱性蛋白酶、胰蛋白酶)分别水解南极磷虾得到多肽的体外抗氧化活性,以清除超氧阴离子、羟自由基和DPPH的能力为指标。结果表明,木瓜蛋白酶水解制备的多肽抗氧化活性比较好,其次是碱性蛋白酶。用响应面法优化木瓜蛋白酶水解南极磷虾制备多肽的工艺参数,经凝胶电泳测定其分子量,显示该多肽有3个明显条带,其分子量大多位于3~7 ku。将上述多肽溶液脱氟后经超滤系统分离,按分子量(molecular weight,MW)大小分为MW≥10 ku,5~10 ku,MW≤5 ku的3种多肽,并比较其抗氧化活性的强度。在本实验体系浓度范围内,3种不同分子量的多肽对超氧阴离子、羟自由基、DPPH的清除能力有所差异,随着多肽浓度的增加,其对清除能力也增大,呈明显的剂量依赖关系。 相似文献
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To further utilize bioactive substance such as bovine colostrum sIgA and IgG,sIgA and IgG were isolated and purified simultaneously by salting out,ultrafiltration and gel chromatography,etc.The analysis of results were showed quantitatively by non-hydrogenized SDS-PAGE,and quanlities of sIgA and IgG were respectively detected by Western Blot.The results showed that the purity and yield of bovine colostrum sIgA were 85.3%and 42.8%,respectively,while the purity and yield of bovine colostrum IgG were respectively 97.2%and 64.4%.This preparative method provides theoretical and experimental foundation for sIgA industrial production. 相似文献
8.
超滤技术对苹果汁加工中主要芳香成分影响的研究 总被引:6,自引:2,他引:6
选用青香蕉苹果和国光苹果为原料,研究了超滤对苹果汁主要芳香成分的影响。苹果汁用国产平板膜超滤机澄清,用顶空动态采样法收集芳香物,用气相色谱法分析芳香成分。通过比较超滤前后青香蕉和国光苹果汁芳香成分的变化,截留分子量为10000daltons的聚砜膜PS—10对苹果汁芳香成分保留有良好效果,优于PS—30,PS—60和PS—80;聚砜膜对醇类芳香成分保留优于酯类芳香成分,并对其截留机理作了初步探讨,认为吸附起主要作用。芳香物回收实验表明,12000Pa,50℃为苹果汁芳香成分回收的适宜条件。 相似文献
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姬松茸子实体多糖的分子量分布研究 总被引:5,自引:0,他引:5
微滤和不同截留分子量的超滤串联,对姬松茸子实体粗多糖进行分级纯化,工艺简单可行,操作方便。可将姬松茸多糖按分子量分成>30万Da、30万~8万Da,8万~1万Da以及<1万Da 4个级别,所得多糖质量分布比例约为5∶1∶3∶1。在低温下干燥制备的姬松茸多糖制品,纯度均高于70%,多糖总回收率高达75.60%。 相似文献
10.
LIU Xiaofei GAO Xuejun YAO Yonghao ZHOU Shenghua 《东北农业大学学报(英文版)》2008,15(2):23-25
sIgA in bovine colostrum was purified by ultrafiltration and enzymolysis methods in this experiment, and the prepared substance was detected by Western Blot. The purity and yield were up to 73.6% and 65.2%, respectively. This convenient technique offers helpful exploration for production of bovine colostrum sIgA 相似文献