膜过滤绿茶多糖的系统分级纯化及免疫活性分析

采用150kD、20kD、6kD的超滤膜和对水溶性茶多糖进行分级和浓缩,得到的三部分截留液分别经过DEAE-52纤维素离子交换层析和丙烯葡聚糖凝胶Sephacryl S-300柱层析系统分离、纯化,共得到了20多个分级组分;HPGPC-ELSD鉴定各多糖组分的纯度及分子量分布,结果表明得到5个均一多糖组分;用小鼠巨噬细胞系Raw264.7检测免疫活性,发现对小鼠巨噬细胞Raw264.7有显著活性的多糖组分大多集中在20kD左右,大多为不均一多糖。     

超滤法浓缩鳀加工蒸煮液的工艺研究

文章以鳀（Engraulis japonicus）蒸煮液为原料,采用聚醚砜超滤膜浓缩回收蒸煮液中有效成分,对样品预处理方法、膜截留孔径的大小、操作压力、进样流速、样品pH与膜通量等的关系进行了研究。结果表明,预处理采用0.2μm的微滤膜过滤,样品中营养物质损失较小且固体颗粒去除彻底;同时,确定了最佳的超滤操作条件为膜孔径30kDa,操作压力137.90kPa,进样流速2.5mL·s^-1,pH8.5,温度30℃。将200mL样品蒸煮液进行浓缩,体积浓缩倍数为1时,蛋白质浓缩倍数为1.61,蛋白质的截留率为80.58%,膜通量达94.4L·（m^2·h）^-1。     

Identification of the Major ACE-Inhibitory Peptides Produced by Enzymatic Hydrolysis of a Protein Concentrate from Cuttlefish Wastewater

The aim of this work was the purification and identification of the major angiotensin converting enzyme (ACE) inhibitory peptides produced by enzymatic hydrolysis of a protein concentrate recovered from a cuttlefish industrial manufacturing effluent. This process consisted on the ultrafiltration of cuttlefish softening wastewater, with a 10 kDa cut-off membrane, followed by the hydrolysis with alcalase of the retained fraction. Alcalase produced ACE inhibitors reaching the highest activity (IC₅₀ = 76.8 ± 15.2 μg mL⁻¹) after 8 h of proteolysis. Sequential ultrafiltration of the 8 h hydrolysate with molecular weight cut-off (MWCO) membranes of 10 and 1 kDa resulted in the increased activity of each permeate, with a final IC₅₀ value of 58.4 ± 4.6 μg mL⁻¹. Permeate containing peptides lower than 1 kDa was separated by reversed-phase high performance liquid chromatography (RP-HPLC). Four fractions (A–D) with potent ACE inhibitory activity were isolated and their main peptides identified using high performance liquid chromatography coupled to an electrospray ion trap Fourier transform ion cyclotron resonance-mass spectrometer (HPLC-ESI-IT-FTICR) followed by comparison with databases and de novo sequencing. The amino acid sequences of the identified peptides contained at least one hydrophobic and/or a proline together with positively charged residues in at least one of the three C-terminal positions. The IC₅₀ values of the fractions ranged from 1.92 to 8.83 μg mL⁻¹, however this study fails to identify which of these peptides are ultimately responsible for the potent antihypertensive activity of these fractions.     

Pilot Investigation of Water Production by Ultrafiltration Process during High Algal Period

On account of the characteristics of water in Taihu during high algal period, the pilot investigation was conducted with the combination of ultrafiltration membrane with coagulation, pre oxidation technology or adsorption technology. The results show that the quality of influent water from the three combination processes: coagulation precipitation ultrafiltration membrane, potassium permanganate coagulation precipitation ultrafiltration membrane, potassium permanganate coagulation precipitation power activated carbon ultrafiltration membrane are preferred, with turbidity lower than 0.1 NTU, algae control in the number of 2.5 × 104/L, and other measured targets meet the drinking water standards (GB 5749—2006). The organic matter of high algal water mainly includes hydrophobic organic compounds which are major factors leading to membrane fouling. The effective pre treatment before ultrafiltration membrane can prevent hydrophobic organic compounding into the membrane pore, which will effectively ease the membrane fouling during the high algal period.     

从蛋清浓厚蛋白中提取溶菌酶技术的研究

通过中空纤维超滤法及亲和凝胶层析法对鸡蛋清中溶菌酶进行提取精制,结果表明蛋清溶菌酶的最佳超滤提取工艺为:连续稀释4倍、超滤温度35℃,料液pH值8.5,进样压力0.08 MPa,再经超滤浓缩处理,酶活得率为131.93%,溶菌酶透过率为73.57%,样品液的酶活约为4 700 U/mL。经过亲和凝胶层析的进一步纯化及真空冷冻干燥,最终蛋清溶菌酶冻干粉的酶活为18 500 U/mg,蛋清溶菌酶的酶活得率为180.83%。     

超滤技术在鲁梅克斯K-1加工中的应用

通过试验研究了在鲁梅克斯K-1的加工中应用超滤技术对其汁液进行浓缩并减少加热提取叶蛋白时的能源消耗的方法。试验表明：采用聚砚中空纤维膜对该作物进行浓缩加工的技术不仅实用，而且节能效果明显。     

A Method of Purification of Bovine Colostrum sIgA

sIgA in bovine colostrum was purified by ultrafiltration and enzymolysis methods in this experiment, and the prepared substance was detected by Western Blot. The purity and yield were up to 73.6% and 65.2%, respectively. This convenient technique offers helpful exploration for production of bovine colostrum sIgA.     

膜技术制备饮用水的应用综述

在综合大量国内外文献的基础上 ,介绍了反渗透、纳滤、超滤、微滤、电渗析等膜技术在制备饮用水上的研究及应用     

Technologic Study on the Isolation of Polysaccharides from Herba Spirodelae seu Lemnae by Ultrafiltration

The aim of the paper is to investigate the technics of isolating the immune-active polysaccharides from Herba Spirodelae seu Lemnae(PHS) by ultrafiltration.PHS were extracted and isolated through decoction,ultrafiltration,(removal) of protein and ethanol precipitation et al.The total contents of saccharides in the extraction and sample were(determined) by the methods of sulphuric acid phenol and sulphuric acid anthrone.The protein contents were determined by colorimetric method of coomassie brilliant blue G 250.Some hazel powder was gained.The purity,the contents of protein,and the recovery are 53.5%,1.2%,and 6.43%,respectively.Ultrafiltraion is a kind of method,which can isolate PHS simply,quickly and effectively.     

亚麻籽中木脂素提取工艺的研究

传统的亚麻籽木脂素提取工艺步骤繁琐,提取物中有益成分散失。研究了溶剂法提取亚麻籽中木脂素的工艺,确定了最佳溶剂系统:质量分数为70%的乙醇,提取温度为室温,时间为24h。建立了中空纤维超滤膜超滤木脂素提取液的平均渗透通量数学模型,并用亚麻籽中木脂素提取液进行了实验验证。结果表明,该工艺步骤简单、易行,超滤模型的计算结果与实验结果基本吻合,所建立的模型可用于超滤过程渗透通量的预测。