Bacteria and antibiotic resistance detection in fractures of wild birds from wildlife rehabilitation centres in Spain

Anatomic adaptations make birds more prone to open fractures with exposed bone parts losing vascularization. As a result of this exposure, fractures are colonized by different microorganisms, including different types of bacteria, both aerobic and anaerobic, causing osteomyelitis in many cases. For this reason, antibiotic treatment is common. However, carrying out antibiotic treatment without carrying out a previous antibiogram may contribute to increased resistance against antibiotics, especially in migratory wild birds. In this paper, bacterial counts regarding fracture type, bacterial identification and antibiotic resistance have been analysed in wild birds from wildlife rehabilitation centres in Spain. The results obtained showed that open fractures had higher bacterial counts (CFU/mL) than closed ones. Bacteria in family Enterobacteriaceae, identified were Escherichia spp., Enterobacter spp., Shigella spp., Hafnia alvei, Proteus mirabilis, Leclercia adecarboxylata and Pantoea agglomerans. Other bacteria present in wild birds’ fractures were Aeromonas spp., Enterococcus spp. Bacillus wiedmannii and Staphylococcus sciuri. All species found presented resistance to at least one of the antibiotics used. Wild birds can be implicated in the introduction, maintenance and global spreading of antibiotic resistant bacteria and represent an emerging public health concern. Results obtained in this paper support the idea that it is necessary to take this fact into account before antibiotic administration to wild animals, since it could increase the number of bacteria resistant to antibiotics.     

野生大豆内生真菌对作物促生作用的研究

为了实现绿色生态农业、农作提质增效,加快生防菌剂的研发进度。本研究利用具有广谱拮抗玉米大斑病和大豆疫霉病野生大豆内生真菌Y6R15和Y2S2为试材,通过菌液灌根和叶喷处理,分析不同处理下玉米和大豆苗期干物质和根系指标的变化。结果表明：拮抗菌株Y6R15对玉米叶片和根干重促生作用明显,均提高根长度、根表面积、根体积、根尖数等指标,以根冠比指标标准Y6R15叶喷效果为最佳方式,依据根系指标特征变化Y6R15灌根效果好于叶喷。拮抗菌株Y2S2和Y6R15处理均对大豆有促生作用,效果显著,Y6R15菌株对玉米和大豆均有促生作用。     

Genetic diversity and antibiotic susceptibility of Staphylococcus aureus isolates from wild boars

We here report the occurrence of S. aureus in wild boars and characterize isolates genotypically and phenotypically in order to get knowledge about the occurrence of clonal lineages and genotypes in free-living wild animals. Forty-one S. aureus isolates obtained from 111 wild boars hunted in Lower Saxony, Germany, were investigated and compared to human and livestock isolates. The S. aureus belonged to multilocus sequence types ST1, ST7, ST30, ST133, ST425, ST804, ST890 and to the new ST3237, ST3238, ST3255 and ST3369. The livestock associated CC398-MRSA lineage, however, was not found. In addition to well-known spa types, the new types t14999, t15000, t15001 and t15002 were detected. Macrorestriction analysis revealed a variety of different SmaI fragment patterns. Most isolates were susceptible to all antimicrobials tested, including methicillin, and resistance was detected only to ampicillin, penicillin and erythromycin. PCR analysis confirmed the presence of staphylococcal enterotoxin genes (seh) in all t127-ST1 isolates. A high degree of genetic diversity was detected with many spa types and clonal lineages previously reported in humans and livestock animals.     

First serological evidence of West Nile virus infection in wild birds in Northern Algeria

While the epidemiology of Flaviviruses has been extensively studied in most of the Mediterranean basin, little is known about the current situation in Algeria. In order to detect the circulation of West Nile (WNV) and Usutu viruses (USUV) in Kabylia, 165 sera were collected from two wild birds species, namely the long distance migrant Turdus philomelos (song thrush) (n = 92) and the resident Passer domesticus (house sparrow) (n = 73). A total of 154 sera were first analyzed by commercial competition ELISA. WNV and USUV micro-neutralization tests were performed on all c-ELISA positive sera and all samples with poor volume. Overall, 7.8 % (CI95 %: 3.5–11.9) were positive by c-ELISA. Positive results were detected in 12.5 % (CI95 %:5.6–19.4) of song thrushes and 1.5 % (CI95 %: 0.0–4.5) for sparrow.Micro-neutralization tests revealed an overall seroprevalence of 6.7 % for WNV (CI95 %: 2.9–10.3), Neutralizing antibodies were found in 8.7 % (CI95 %: 3.0–14.4) for song thrushes and in 4.1 % (CI95 %: 0.0–8.7) of sparrows. The current study demonstrates significant seroprevalence of WNV antibodies in wild birds in Algeria.     

Micropropagation of Prunus scoparia,a Suitable Rootstock for Almond under Drought Conditions

Prunus scoparia is a wild deciduous shrub, usually living on dry calcareous soils of the rocky mountains and has been used as a grafting rootstock for domesticated almonds to provide drought resistance. In the current study, micropropagation ability of P. scoparia was investigated using cytokinin and auxin. Uniform nodal shoot pieces (3–5 cm in length) of seedlings were used as explants. The explants were disinfected with 10% sodium hypochlorite solution. For adventitious shoot induction and proliferation, Murashige and Skoog (MS) media containing 7.00 g/l agar and 30.00 g/l sucrose containing five concentrations of benzyl adenine (BA) (0.00, 0.50, 1.00, 2.00, and 4.00 mg/1) and also containing six concentrations of Thidiazuron (TDZ) (0.00, 0.50, 1.00, 2.00, 5.00, and 7.00 mg/1) were compared. For rooting, in vitro shoots (2–3 cm) were transferred into ½ MS medium supplemented with 30 g/l sucrose, 7.50 g/l agar, and different concentrations of IBA (0.00, 0.25, 0.50, and 1.00 mg/l) and NAA (0.00, 0.25, 0.50, and 1.00 mg/l). Based on the results obtained for shoot proliferation, only 2.00 and 4.00 mg/l BA and 2.00 mg/l TDZ concentrations generated shoots, while other treatments did not show shoot proliferation. Among the three treatments that generated shoots, the best results for shoot number, leaf number, and leaf color quality were observed in media containing 2.00 mg/l TDZ. Based on the results obtained for rooting, the effect of IBA concentrations on the rooting percentage, root number, and root length was significant. Among IBA concentrations, only 0.50 mg/l IBA induced rooting, while there was no rooting in the media containing other IBA concentrations. None of the NAA concentrations showed rooting. In conclusion, MS culture medium supplemented with 2.00 mg/l TDZ and ½ MS culture medium supplemented with 0.50 mg/l IBA are suggested for in vitro shoot proliferation and rooting of P. scoparia, respectively. The results presented herein could be used for in vitro selection and micropropagation of P. scoparia.     

Wild Bonelli’s eagles (Aquila fasciata) as carrier of antimicrobial resistant Salmonella and Campylobacter in Eastern Spain

Wild birds have repeatedly been found to be involved in the dissemination of enteric bacterial pathogens in the environment. The aim of this study was to determine the occurrence of Salmonella and Campylobacter as well as the antimicrobial resistance in wild Bonelli’s eagles nestlings in Eastern Spain. In addition, we compared the efficiency of two sampling methods (fresh faecal samples from nest and cloacal swabs from nestlings) for detection of both bacteria. A total of 28 nests with 45 nestlings were analysed. In the nest, Salmonella occurrence was 61 ± 9.2%, while Campylobacter occurrence was 11 ± 5.8% (p < 0.05). In the nestlings, Salmonella occurrence was 36 ± 7.1%, while Campylobacter occurrence was 11 ± 4.7% (p < 0.05). Eight Salmonella serovars were identified, and the most frequently isolated were S. Enteritidis, S. Typhimurium, S. Houston, and S. Cerro. Only one Campylobacter species was identified (C. jejuni). Regarding antimicrobial resistance, the Salmonella strains isolated were found to be most frequently resistant to ampicillin and to tigecycline; however, the sole Campylobacter strain recovered was multidrug resistant. In conclusion, this study demonstrated that wild Bonelli’s eagles nestlings are greater carriers of Salmonella than of Campylobacter. Both Salmonella and Campylobacter isolates exhibited antimicrobial resistance. In addition, faecal samples from nests were most reliable for Salmonella detection, while cloacal swab from nestlings were most reliable for Campylobacter detection.     

乡土植物在城市绿化中的应用

介绍乡土植物在城市绿地系统中应用的重要性,分析了乡土植物在城市绿地中的生态、景观、经济等方面的作用,认为人类生存必须依附于自然,城市的建设要尽最大可能减少对自然的破坏,增加乡土植物应用的比例,建立健康的生态系统。同时根据植物景观的需要,介绍了部分在城市绿地有应用前景的野生植物类型。     

中国国家重点保护野生植物现状

国家林业局于1996年始在全国范围内组织了国家重点保护野生植物资源调查，历时5年，被调查的野生植物物种共191种(含变种)。调查表明，盐桦(Betula halophila)、金平桦(Betula jinpingensis)、秤锤树(Sinojachia xylocarpa)3树种此次调查未找到。原产地野生仅存1～10株的木本植物有12种：普陀鹅耳枥(Carpinus putoensis)、绒毛皂荚(Gleditia japonica var.velutina）、丹霞梧桐(Firmiana danxiaensis)、广西火桐(Erythropsis kwangsiensis)、百山祖冷杉(Abies beshanzuensis)、羊角槭(Acer yangjuechi)、云南蓝果树(Nyssa yunnanensis)、猪血木(Euryodendron excelsum)、天目铁木(Ostrya rehderiana)、华盖木(Manglietiastrum sinicum)、滇桐(Craigia yunnanensis)、膝柄木(Bhesa sinensis)。原产地野生仅存11～100株的木本植物有9种：峨眉拟单性木兰(Parakmeria omeiensis)、天台鹅耳枥(Carpinus tientaiensis)、景东翅子树(Pterospermum kingtungense)、巧家五针松(Pinus squamata)、紫檀(Pterocarpus indicus)、梓叶槭(Acer catalpifolium)、异形玉叶金花(Mussaenda anomala)、广西青梅(Vatica guangxiensis)、毛枝五针松(Pinus wangii)。仅存50000株以下的国家重点保护的野生植物共有85种，占191种的44．5％，形势异常严峻。报告分析了不同区域和空间野生植物分布的差异、存在的问题，并提出了5点建议。     

贵州亚热带地区的野生香花类观赏植物资源

贵州亚热带地区有野生香花类观赏植物23科39属（1亚属）70种（含新分布种和变种）。分布垂直带谱为海拔160－1000m。花色分为白色、黄色、紫色、黄绿色和红色5类。生境类型分为森林湿地型、疏林灌丛型、山坡草地型和附生型，以森林湿地型和疏林灌丛型的种类居多。银钟花、大叶木莲、尖叶木、云川醉鱼草、紫萼、香槐、两粤黄檀、单性木兰、苍白秤钩风和扭肚藤处于濒危状态。     

湖南野生观赏植物及其园林应用

湖南坐落在温带和中亚热带的植物过渡地区,拥有维管束植物5 500余种(含种下单位)。以现有欧亚和北美栽培的观赏植物为基础,湖南有2 000多种野生植物可作为栽培观赏植物,有很高的观赏价值和选育潜力。根据它们的观赏特性、抗寒性、选育潜力与园林应用的不同,可将湖南观赏植物划分为10类。将观赏植物引入园林建设,对创建地带性、个性化的园林景观具有特别重要的意义。