Role of PhoPR in the response to stress of Mycobacterium bovis

PhoP is part of the two-component PhoPR system that regulates the expression of virulence genes of Mycobacteria. The goal of this work was to elucidate the role of PhoP in the mechanism that Mycobacterium bovis, the causative agent of bovine tuberculosis, displays upon stress. An analysis of gene expression and acidic growth curves indicated that M. bovis neutralized the external acidic environment by inducing and secreting ammonia. We found that PhoP is essential for ammonia production/secretion and its role in this process seems to be the induction of asparaginase and urease expression. We also demonstrated that the lack of PhoP negatively affected the synthesis of phthiocerol dimycocerosates. This finding is consistent with the role of the lipid anabolism in maintaining the redox environment upon stress in mycobacteria. Altogether the results of this study indicate that PhoP plays an important role in the response mechanisms to stress of M. bovis.     

Clostridioides difficile in bat guano

Bats are associated with the emergence of several mammalian diseases. Their sessional migration, and tendency to form large colonies in close proximity to human habitats enables effective intra- and inter-species transmission of pathogens. Clostridioides difficile is an important enteric pathogen in humans and animals; however, the source of its dissemination in the population is unknown. The purpose of this study was to determine the prevalence of C. difficile in bats, and to characterize C. difficile isolates.Feces (n = 93) was sampled from bats during their migration across Europe. Eighteen samples (19.4%) were positive for C. difficile; ribotypes 078, 056, and a new ribotype CDB3 were identified.Clostridioides difficile ribotypes 078 and 056 are associated with human and animal diseases. The C. difficile prevalence and ribotypes in this study do not necessarily identify bats as a significant source, but more likely as an indicator of C. difficile perpetuation in the environment.     

Impact of soil water content on maize responses to the plant growth-promoting rhizobacterium Azospirillum lipoferum CRT1

Members of the bacterial genus Azospirillum are root-associated bacteria that increase yield in cereals by promoting growth and alleviating drought stress. How plants integrate the many bacterium-derived growth-promoting stimuli with other environmental factors to generate a coordinated response remains unresolved. Using a commercial Azospirillum strain, A. lipoferum CRT1 and two host maize cultivars, it was observed that bacterization reduced the drought-induced increase in lateral root growth and enhanced the flood-induced increase in lateral root growth in the more drought- and flood-sensitive cultivar. In the other one, A. lipoferum CRT1 only elicited a moderate root growth response under low soil water potential. The photosynthetic potential and activity were increased in the earlier cultivar and decreased in the later one, irrespective of the soil water content. No impact of the bacterium was seen on the growth of the leaves of both cultivars under both stresses until the third leaf stage, therefore suggesting that it is a consequence of multiple primary adaptations to biotic and abiotic stresses. It is suggested that host–bacteria recognition leads to a stress-specific modulation of the root response and a differential stress-independent effect on photosynthesis. This is the first report of the impact of Azospirillum under flood conditions.     

Evaluation of cytokines and sialic acids contents in horses naturally infected with Theileria equi

This study was undertaken to assess the effects of T. equi infection on serum concentrations of some important cytokines including interferon-γ (IFN-γ), tumor necrosis factor-α (TNF-α), and interleukin-1 beta (IL-1β), IL-1α, IL-4, IL-6, IL-8, IL-10, IL-12α, IL-12β, IL-18, as well as total, protein and lipid binding sialic acids (TSA, PBSA and LBSA). Furthermore, any probable relation among the parasitemia, cytokines and sialic acids (SAs) were calculated using Pearson correlation and simple linear regression. Almost 300 draft horses (Kurdish-breed) with age of 3–4 years old from north-west of Iran were examined and an infected group comprised of 28 mares, naturally infected with T. equi, was identified and divided into 3 subgroups according to their parasitemia rates (low <1 %, moderate 1–3 % and high 3–5 %). Twenty healthy horses were considered as a control. Characterization and differentiation of piroplasmosis were conducted using routine hematological procedures and specific PCR assay. The results revealed a significant increase (P < 0.05) in all of the cytokines and SAs in a parasitic burden-dependent fashion. Additionally, a strong and positive relation was detected among the parasitemia, cytokines and SAs. Conclusively, T. equi infection is associated with induction of severe inflammatory processes in horses and SA plays a pivotal role in pathophysiology of the disease as it is tightly correlated with the parasitemia rate.     

Proteomic analysis of soluble protein extract of adult Toxocara cati

Toxocara cati is a cat roundworm and the causative agent of toxocariasis as a cosmopolitan zoonotic disease. As no information has been reported so far, identification of T. cati proteins can be useful for the development of new diagnostic strategies. This study was conducted to identify the major proteins in the adult T. cati tegument using bi-dimensional electrophoresis (2-DE) and shotgun proteomics. A total proteins were identified, among them the metabolic enzymes were the largest group, including: Enolase, triose phosphate isomerase, fructose-bisphosphate aldolase, aldehyde dehydrogenase. The other important protein groups recognized in T. cati, belong to the HSP-family, the structure and motor proteins, such as actin. The role of these proteins have been implicated in parasite–host interactions and modulating cellular immune response, immune regulation in evasion mechanisms of the host immune response. Characterizing T. cati adult proteins play a key role not only in host-parasite interactions, but also in the discovery of drug targets, subunit vaccines against toxocariasis, immunodiagnostic kits for toxocariasis and the identification of novel immuno-modulators that can form the next generation of therapeutic possibilities for inflammatory diseases.     

Genetic differentiation of Mycobacterium bovis and Mycobacterium tuberculosis isolated from cattle and human sources in,Egypt (Suez Canal area)

Bovine tuberculosis is a devastating illness in cattle and it has the ability to transmit causing severe troubles in human. Mycobacterium bovis (M. bovis) infection in human indeed becomes increasingly critical especially in developing countries. Early diagnosis is very important to control and limit its spreading. The aim of this study is to examine the genetic differentiation and possibilities of transmission between cattle and human. Lymph node and sputum samples were collected from cattle and patients showing tuberculin test positive; respectively for phenotypic identification and for molecular examination by detection of IS6110 and oxyR genes which are specific for MTC and M. bovis; respectively. The phenotypic identification of sputum samples showed 80 % positive by both stain and culture, while, lymph nodes revealed 66 % and 84 % positive by stain and culture method; respectively. Alignment of oxyR gene sequences of M. tuberculosis and M. bovis was used as a feature for differentiation between the 2 genes in these two genetically closely similar microorganisms showed 99 % identities between the 2 genes. Alignment and phylogenetic analysis of Mpb70 gene sequences from animal and human origin showed very high relatedness (99.32 %) to each other confirming that the zoonotic transmission is most probably occurred.     

Factors driving natural regeneration beneath a planted urban forest

Cities around the world are investing in urban forest plantings as a form of green infrastructure. The aim is that these plantations will develop into naturally-regenerating native forest stands. However, woody plant recruitment is often cited as the most limiting factor to creating self-sustaining urban forests. As such, there is interest in site treatments that promote recruitment of native woody species and simultaneously suppress woody non-native recruitment. We tested how three, common site treatments—compost, nurse shrubs, and tree species composition (six-species vs. two-species)—affected woody plant recruitment in 54 experimental plots beneath a large-scale tree planting within a high-traffic urban park. We identified naturally regenerating seedling and sapling species and measured their abundance six-years after the site was planted. This enabled us to examine initial recruitment dynamics (i.e. seedlings) and gain a better understanding of seedling success as they transition to the midstory (i.e. saplings). Seedling and sapling recruitment (native and total) was greater in areas with higher canopy cover. The combination of the nurse shrub treatment with compost and species composition (six-species) treatments increased seedling recruitment by 47% and 156%, respectively; however, the nurse shrub treatment by itself decreased seedling recruitment by 5% and native seedling recruitment by 35%. The compost treatment alone had no effect on the total number of recruits but resulted in 76% more non-native seedlings. The sizes of these treatment effects were strongly dependent on whether the forest plantings were in open areas, versus areas with existing tree canopy, the latter condition facilitating recruitment. Our findings therefore suggest that combinations of site treatments, paired with broad canopy tree species, may be most effective for promoting regeneration of native species resulting in more self-sustaining urban forests.     

Most fish destined for fishmeal production are food‐grade fish

Marine fisheries target and catch fish both for direct human consumption (DHC) as well as for fishmeal and fish oil, and other products. We derived the fractions used for each for 1950–2010 by fishing country, and thus provide a factual foundation for discussions of the optimal use of fisheries resources. From 1950 to 2010, 27% (~20 million tonnes annually) of globally reconstructed marine fisheries landings were destined for uses other than DHC. Importantly, 90% of fish destined for uses other than DHC are food‐grade or prime food‐grade fish, while fish without a ready market for DHC make up a much smaller proportion. These findings have implications for how we are using fish to feed ourselves or, more appropriately, how we are not using fish to feed ourselves.