不同蛋白水平下添加抗菌肽对赤点石斑鱼影响的研究

评估了不同饲料蛋白水平(44%,48%,52%)和抗菌肽浓度(0.01%,0.02%)对赤点石斑鱼生长性能、血清生化、消化酶和抗氧化能力的影响。实验组分别为:D1(44%,0.01%),D2(48%,0.01%),D3(52%,0.01%),D4(44%,0.02%),D5(48%,0.02%),D6(52%,0.02%)。每组3个平行,养殖8周结果如下:一定范围内,增重率、特定生长率和饲料转化率随蛋白水平和抗菌肽浓度增加,D5组显著高于D1组(P0.05)。形态学指标,D6组肝体比(HSI)、脏体比(VSI)和肠系膜脂肪(IPF)显著低于D5组(P0.05)。同一蛋白水平下增加抗菌肽浓度可降低肌肉粗脂肪含量,D4组显著低于D1组(P0.05)。血清总蛋白、白蛋白含量随蛋白水平和抗菌肽浓度的增加而升高(P0.05),D3和D5组谷丙转氨酶、谷草转氨酶和血糖显著低于其他组(P0.05)。D5组胰蛋白酶高于其他组(P0.05),D1组过氧化物酶显著低于其他组(P0.05)。随着抗菌肽浓度增加,胃蛋白酶、过氧化物酶、过氧化氢酶活性增加,且丙二醛含量降低(P0.05)。实验结果表明,48%蛋白水平下添加0.02%抗菌肽可以提高赤点石斑鱼生长性能,增强消化酶活性和抗氧化能力,降低饲料系数。     

我国农村居民消费问题研究

采用线性回归方法分别测算了农村和城市居民的边际消费倾向,从制度因素、基础设施、营销工作、信贷环境、群体影响等方面分析了农村居民消费倾向较低的原因,提出了提高农村居民边际消费倾向的思路。     

广州市海珠区果树保护区鸡生态学研究

鸡（荔枝菌）是广州市海珠区果树保护区极具地方特色的野生食用菌,其生态类型是荔枝菌与白蚁共生共衰的＂蚁栽＂食用菌,属蚁巢伞属（Termitomyces）,在雨量充沛（旬均雨量132mm、旬均日照时数28.8h）、高温、高湿（旬均气温28.9℃、旬均湿度80.3%、旬均地温28.6℃）的6月出产,其中尤与相对湿度关系密切。荔枝菌菌圃含水量高（20.74%）,呈酸性（pH值4.38）,且营养丰富,主要为糖和蛋白质,分别达到40.38%和6.27%。     

Assessment of affecting factors in measuring activating platelets with the method of flow cytometry

AIM:To investigate the affecting factors of detecting platelet activation by flow cytometry (FCM).METHODS:Using decoagulant of natrium citricum, anticoagnlated peripheral venous bloods from 6 healthy donors were labeled with the method of three-colour immunofluorescence assay.Platelet activation markers fibrinogen receptor (Fib-R, PAC-1) and P-selectin (CD62P) were measured.In the same time, the reproducibility of FCM was assessed.RESULTS:The platelet activation markers PAC-1 and CD62P at each time point showed significant difference(P<0.05).The ratio was increased with time extending.The positive ratio of PAC-1 and CD62P immediately measured (within 10 min) was 2.7% and 3.5% less than those at time point of 30 min.The results were measured several times under different activation levels.The coefficient of variation was less than 5%.CONCLUSION:In room temperature and with decoagulant of natrium citricum, if the measurements of PAC-1 and CD62P are finished within 30 min after sampling, good reproducibility should be achieved.     

油菜不育系与菜心杂种后代的品种选育

进行了油菜不育系与菜心杂种后代的品种选育研究。通过对BC3代群体植株品质指标总糖、还原糖、蛋白质、维生素C含量的测定及生长优势观测,研究了杂种后代的品质特性。结果表明:BC3代群体植株具有了较好的营养品质,还原糖、总糖的超亲优势分别为40.39%和39.51%;蛋白质、维生素C含量的超亲优势分别为16.67%和21.82%;植株BC3代也具有侧芽生长快,收获期长,外形趋于菜心等生长优势,具备了选育的价值。     

食物和环境中氟乐灵农药残留检测方法研究进展

氟乐灵农药是一种二硝基苯胺类芽前土壤处理除草剂,在食物和环境中残留严重,会对人体产生危害。主要总结了氟乐灵的残留限量,通过综述色谱法、免疫分析方法、电化学法、光度计法、生物检测法等5种氟乐灵检测方法进展情况,并对氟乐灵检测技术的发展进行展望,为氟乐灵残留检测技术的进一步研究提供参考。     

珍禽贵妃鸡与广西三黄鸡杂交后代的外貌特征及生长性状研究

选用广西三黄鸡为母本、贵妃鸡为父本,按照试验设计和规定指标,进行杂交,选育杂交后代,再以回交方法固定目标性状,培育至杂交F3代,并对杂交F3代进行外貌特征和生长性状(体重、屠宰性能)的研究,比较杂交F3代与贵妃鸡的异同,研究杂交F3代的外貌特征与生长发育规律。结果表明,贵妃鸡与广西三黄鸡的杂交后代外貌特征为全身黑羽,其生长速度明显优于贵妃鸡,但仍具有贵妃鸡肉质优良的特点。     

金线莲菌根结构显微观察及超微结构研究

采用徒手切片对金线莲组培苗及共生苗底部茎端进行显微观察,可见金线莲组培苗茎端皮层细胞中无菌丝团,而共生苗皮层细胞中可见大量刚形成或已被消解的菌丝团,表明共生真菌与金线莲组培苗已成功建立共生关系。采用电镜对金线莲共生苗作进一步观察,可见植物细胞中菌丝周围分布较多被分解的淀粉粒及溶解酶,表明共生菌株初期利用了植物的碳源物质,但最终被溶解酶消解,为植物提供营养。     

植物内生真菌多样性及其共生作用

植物内生真菌是生活在健康的植物体内不引起寄主植物任何病害的一类微生物。内生真菌具有丰富的多样性,在与寄主植物协同进化的过程中,通过不同方式为寄主提供有利保护。人们在对内生真菌的研究利用中,可根据研究的需要寻找特殊的内生真菌来源,为生产和研究提供最具研究价值和使用价值的资源。     

Effects of NGF on collagen secretion and morphology of hepatic stallete cells

AIM: To investigate the effects of nerve growth factor (NGF) on the changes of collagen secretion and morphology of hepatic stallete cells(HSCs). METHODS: Rat HSCs were incubated with different concentrations of NGF for 24 h. Collagen I and III in the supernatants of culture medium secreted by HSCs were detected by enzyme-linked immunosorbent assay. The morphological changes of HSCs were observed under inverted microscope with acridine orange staining and under transmission electronic microscope. RESULTS: When HSCs was incubated with NGF at concentrations of 100, 200 or 400 μg/L for 24 h, the content of collagen I and collagen III in the culture supernatants were significantly reduced compared with control group (P<0.05). After stimulated with NGF at the concentration of 100 μg/L for 24 h, the growth of the HSCs was inhibited and the morphous of the cells became round or oval gradually. The morphological changes of apoptotic cells were also observed by acridine orange staining and transmission electronic microscopy. CONCLUSION: NGF inhibits HSCs to synthesize collagen I and collagen III. Inhibition of collagen production and promotion of apoptosis in HSCs may be the possible mechanisms of NGF to reverse liver fibrosis.