甘露糖修饰的壳聚糖聚乳酸-羟基乙酸共聚物纳米微球作为口蹄疫病毒核酸疫苗递送载体的特性评价

旨在对制备的甘露糖修饰的壳聚糖聚乳酸-羟基乙酸共聚物[poly （D，L-lactide-co-glycolide），PLGA]纳米微球作为口蹄疫病毒（foot-and-mouth disease virus，FMDV）核酸疫苗递送载体进行评价。采用西佛碱反应和元素分析制备具有一定取代度的甘露糖修饰的壳聚糖衍生物（mannose modified chitosan，MCS），然后，经双重乳化挥发法制备得到甘露糖修饰的壳聚糖PLGA纳米微球（MCS-PLGA-NPs）。采用纳米粒径仪检测MCS-PLGA-NPs粒径分布和表面电势（zeta）、扫描电镜考察其形态、琼脂糖凝胶电泳观察其对质粒的吸附和吸附质粒后抵抗核酸酶降解能力、CCK-8法检测MCS-PLGA-NPs的细胞毒性、激光共聚焦观察巨噬细胞对MCS-PLGA-NPs-质粒DNA复合物的摄取、荧光显微镜和Western blot验证MCS-PLGA-NPs加载质粒DNA在细胞中的表达。元素分析结果表明，成功制备了取代度为5%~10%的MCS。纳米粒径测定和扫描电镜结果表明，MCS-PLGA-NPs的zeta为正值、粒径分布均匀且形态规则呈球形。琼脂糖凝胶电泳结果显示，MCS-PLGA-NPs吸附质粒的能力随着其质量的增加而增强并且可以在一定程度上抵抗核酸酶降解质粒DNA。在细胞毒性试验中，不同浓度的MCS-PLGA-NPs与RAW264.7细胞共孵育24 h后，细胞存活率仍在85%以上。在细胞摄取试验中，用激光共聚焦显微镜可以明显观察到质粒DNA结合到纳米微球表面被RAW264.7细胞摄取。荧光显微镜和Western blot试验证明MCS-PLGA-NPs加载质粒DNA可以在细胞中进行表达。综上表明，本研究成功制备了MCS以及具有递送核酸疫苗能力的MCS-PLGA-NPs，为FMDV核酸疫苗的递送研究提供了新的方向和见解，也为该递送载体携带特定抗原靶向抗原递呈细胞表面甘露糖受体以及应用于动物免疫的研究奠定基础。     

Preparation of Alginate-Based Biomaterials and Their Applications in Biomedicine

Alginates are naturally occurring polysaccharides extracted from brown marine algae and bacteria. Being biocompatible, biodegradable, non-toxic and easy to gel, alginates can be processed into various forms, such as hydrogels, microspheres, fibers and sponges, and have been widely applied in biomedical field. The present review provides an overview of the properties and processing methods of alginates, as well as their applications in wound healing, tissue repair and drug delivery in recent years.     

农药有效利用率与喷雾技术优化

提高农药的有效利用率是植保工作者非常关心的问题。本文阐述了农药有效利用率的广义和狭义涵义,并分析了农药使用中存在的药剂浪费、有效利用率低的问题。根据喷雾技术中的“剂量传递”过程,分析了农药有效利用率的狭义涵义,在春季果园和作物苗期,常规喷雾法的农药有效利用率只有20%~30%;在夏秋季果园和作物中后期,随着作物叶面积系数（LAI）的增加,农药的有效利用率可达到50%~60%。论文分析了造成农药有效利用率低的原因,提出喷雾技术的优化措施：（1）大田喷雾时采用机动喷杆喷雾替代背负式手动常规喷雾,可以改善雾滴沉积分布的均匀性;（2）添加喷雾助剂可以提高药液在靶标表面的润湿性;（3）优化雾滴粒径,采用细雾滴替代粗雾滴可以提高雾滴的中靶率;（4）降低施药液量可以减少药液流失;（5）加装挡板可以减少雾滴飘失等。通过以上技术的优化,可以大幅度提高农药的有效利用率,达到减量增效的目的。     

Marketing mix for the promotion of biological control among small-scale paddy farmers

Understanding farmers’ decision process on the adoption of biological control (BC) may help in promoting adoption. This study explored for the first time the impact of marketing mix elements (product, price, promotion, and place) on bolstering BC for the management of rice stem borer (Chilo suppressalis Walker) among rice farmers in Fuman County in northern Iran. Farmers using BC were familiar with this method primarily via village councils and rural municipality organizations (Dehyaris) and then via distributors of trichocards. Almost half of the farmers (48.3%) had weak to moderate level of contact with distributors of trichocards. All four elements (4Ps) of marketing mix (product, place, price, and promotion) were effective on the marketing of BC agents. Place (distribution) mix, followed by price mix, were the most important factors in marketing trichocards in farmers’ views. According to the sensitivity to 4Ps of marketing mix, most farmers were grouped as semi-sensitive (45.6%) and sensitive (39.4%). Overall, the availability of biological agent products in the market, the affordable price of the products, and the strong relationship of rural people with village councils and rural municipality organizations (Dehyaris) are crucial factors for BC promotion in paddy fields of Fuman.     

基于柔性夹持的青菜头收获机设计与试验

为解决西南丘陵山地青菜头机械化收获问题，结合青菜头生物特性和农艺要求，设计了一种具有柔性夹持功能的小型青菜头收获机。阐述了整机结构与工作原理，并对样机关键部件进行了结构设计和理论分析，柔性夹持装置初始间隙为60mm，最大允许通过青菜头直径为150mm，分析得到夹持输送带速度为0.37m/s、所需最大夹持力为38.04N；对割台架的仿地形能力和结构动力特性进行了分析，可得该结构允许地形起伏度为50mm，在安装割刀位置振幅最大，前5阶固有频率为115.63~783.60Hz，远大于发动机和地形产生的激励频率，因此不会发生共振现象。田间试验结果表明，切割及夹持输送机构运行平稳、振动小、切割有力、夹持力度适中；切割成功率为89.5%，青菜头损伤率为10.8%，实际工作效率为0.035hm2/h，各项性能指标基本满足设计和农艺技术要求。     

基于WSN的集中通风式分娩猪舍环境参数时空分布特性

为研究集中通风式分娩猪舍环境参数的时空分布特性,以云南省某规模化种猪场为试验对象,利用无线多源多点远程监测系统(Wireless Sensor Network, WSN),对地沟管道进风、中央排风式分娩母猪舍养殖环境开展了环境参数监测试验,并进行统计分析。试验结果表明,在试验期间内,分娩舍内不同时间段之间的温度(00:00-06:00、06:00-12:00、12:00-18:00以及18:00-00:00的平均温度分别为22.98、23.78、24.44和23.61℃)具有显著性差异(P0.05),相对湿度与温度随时间变化呈现负相关性,相对湿度的峰值点为82.31%,温度场分布均匀性比湿度场好,NH_3浓度分布均匀性比CO_2浓度差。NH3浓度在舍内能被有效的维持在低水平范围,各监测区域的浓度远小于指标值(20 mg/m~3),NH_3和CO_2会呈现出在边角积聚现象。温湿度指数较低,舍内平均温湿度指数(Temperature-humidity Index,THI)水平的峰值点为75.30,生猪出现热应激的概率较低。利用WSN系统,对集中通风式分娩舍环境参数进行多源多点远程监测,分析分娩舍环境参数时空分布特性,为分娩舍通风模式与环境调控策略优化设计提供参考。     

芦苇和白刺空间格局对青土湖生态输水的响应

石羊河尾闾湖——青土湖位于腾格里沙漠和巴丹吉林沙漠边缘,是阻隔两大沙漠合围的生态屏障。自2010年开始连续5年向青土湖进行生态输水并形成一定规模的水面,对促进区域植被恢复和保护民勤湖区绿洲具有重要的生态意义。本研究选用2008年、2010年、2011年、2012年、2013年、2014年的高分辨率遥感影像资料同时结合研究区的实地调查资料,对形成人工水面的面积和输水前后的芦苇(Phragmites australis)、白刺(Nitraria tangutorum)群落面积进行了统计分析和对比。结果表明,5年连续输水形成叠加效应,水面面积成倍增加,芦苇相对频度逐年增加和白刺相对频度逐年减少,芦苇群落(Form.P.australis)面积增加了8.43倍,临接水域和水域中的白刺群落(Form.N.tangutorum)消失,白刺面积减少了68.00%。人工输水促进了芦苇群落依水蔓延,镶嵌于水域成片分布;距水面50 m范围的白刺群落由连续成片成点状。输水促进了湿生植物生长,导致荒漠植物种退化。     

加强图书馆联合参考咨询系统的建设,促进文献资源的开发利用

本文简述了文献传递服务发展的基本过程,概括了联合参考咨询网服务模式与内容,对现阶段UCDRS存在的问题进行分析与探讨,提出了解决问题的方法及优化措施,积极整合各类图书馆信息资源和服务力量,促进公益性联合参考咨询系统的发展。     

Performance assessment of small irrigation schemes along the Mauritanian banks of the Senegal River

Irrigation plays a fundamental role in world food provision but, to date, it has performed below expectations in Sub-Saharan Africa. The present study assesses and diagnoses the performance of 22 small and medium size community-managed irrigation schemes, mainly devoted to rice production, in different locations along the Mauritanian banks of the Lower Senegal River. The evaluations followed the Rapid Appraisal Process in which semi-structured interviews were held with representatives of the Cooperatives’ Boards in charge of each scheme to obtain information about the organisation of the cooperative, land tenure, irrigation system and organization, cropping pattern and soils. Additionally, for each irrigation scheme, the water-delivery service was characterized by making qualitative and comparative observations during field inspections; the pumping station's performance was diagnosed by a local specialist; the discharge at the head of the system was measured; daily irrigation time was recorded; and crop yields were determined by plot sampling. Then a set of performance indicators was computed. Water delivery capacity referred to irrigated areas was insufficient in a third of the schemes, and this insufficiency was exacerbated by poor maintenance. Irrigation intensity in habilitated areas was rather low being less than 0.66 in 50% of the schemes. The average productivity of land, irrigation water, and fuel (3.38 t ha⁻¹, 0.30 kg m⁻³ and 2.37 kg kWh⁻¹, respectively) were well below potential.