哈尔滨市儿童公园入口区景观改造探讨

以哈尔滨市儿童公园入口区景观改造设计为例,针对改造前公园入口区景观的不足点,分别对公园大门、出入口、入口区广场等几大部分进行景观改造设计,并对改造后特色进行分析,旨在构建富有北方地域特色的儿童公园新形象。     

沿海地区直播水稻高产栽培技术

介绍了沿海地区水稻直播栽培技术,包括品种选择、茬口安排、大田准备、播种、肥水管理、病虫草害防治和收获等方面内容,以期促进水稻直播栽培技术的推广。     

滨湖稻区大棚甜瓜高产栽培技术

从选用优良品种、播种育苗、定植、田间管理、植株管理、花果管理、病虫害防治、适时采收等方面介绍了滨湖稻区大棚甜瓜高产栽培技术,以期为瓜农提供参考。     

沿海地区无籽西瓜栽培技术

根据无籽西瓜的生育特性及沿海地区的气候特点,介绍了其栽培技术,包括品种选择与种子处理、苗床准备、育苗移栽、定植、肥水运筹、中后期管理等内容,以期指导无籽西瓜高效生产。     

武夷山红壤、黄红壤与黄壤参比及合理开发利用

选取了武夷山红壤、黄红壤与黄壤的5个代表性剖面,采样进行室内理化性质分析。通过实地观察及各项指标的对比分析,结果表明：随着海拔的升高依次分布红壤、黄红壤和黄壤;从红壤→黄红壤→黄壤,有机质累积逐渐增加,而粘粒含量、黏化率及粉粘比逐渐减小。在上述理化性质参比分析的基础上,对武夷山保护区红壤、黄红壤及黄壤的合理开发利用作了简要分析。     

沿江稻区褐飞虱的抗药性现状、分析及用药策略

褐飞虱是水稻上的重要害虫。通过对和县地区褐飞虱对吡虫啉,噻嗪酮、毒死蜱等8种药剂的抗药性系统监测和检测,明确了沿江稻区褐飞虱的抗药性水平,分析了抗性发展动态,并在此基础上提出了沿江稻区褐飞虱的用药治理策略。     

Expression and Characterization of a Thermostable Xylanase Gene xynA from a Themophilic Fungus in Pichia pastoris

The gene of xylanase (xynA) was amplified by RT-PCR from the total RNA of a themophilic fungus Thermomyces lanuginosus SY2. The sequence analysis showed that gene coding region of mature peptide contained 0.585 kb, which coded 194amino acids. The putative amino acid sequence and DNA sequence of xylanase from T. lanuginosus SY2 (GenBank no.:GU166389) were 98.97 and 99.49% identical to the other T. lanuginosus (GenBank no.: U35436). A recombinant plasmid pPIC9K-xynA was constructed by inserting gene xynA into Pichia pastoris secretory vector pPIC9K. Linearized pPIC9K-xynA was transformed into P. pastoris GS115 with the method of electroporation. The recombinant strain was identified by G418 selection and confirmed by PCR analysis. It was induced by 1.0% methanol at 28℃ to express the recombinant xylanase. The results showed that the recombinant xylanase was secreted into extracellular fermentation liquid. The highest enzyme activity of 113.5 IU mL-1 and protein content of 889.7 μg mL-1 were detected for 216 h of induction. The optimal pH value and temperature of the enzyme activity was 5.5 and 65℃, respectively. The xylanase activity retained above 80% from pH value 2.5 to 8.5 for 48 h. The enzyme activity was above 85% at incubation temperature of 55℃.     

我国蔗区推广温水脱毒“健康种苗”生产技术

目前,被农业部作为现代甘蔗糖料生产基地建设和甘蔗高产创建主推技术的甘蔗温水脱毒种苗生产技术,已开始在我国甘蔗产区推广应用,2010年云南、广西蔗区推广温水脱毒"健康种苗"近0.67万hm2。     

施氮量对高海拔冷凉区啤酒大麦产量和品质的影响

在河西高海拔冷凉区进行了氮肥施用量对啤酒大麦产量和品质的影响试验,结果表明,当施纯氮量为120kg/hm<'2>时,啤酒大麦产量达最高,为6 040.0 kg/hm<'2>,蛋白质含量为130.4 g/kg,饱满粒高于80%.     

玉米品种金凯3号在陇东旱塬区的密度试验

在平凉市黄土旱塬区的试验结果表明,玉米品种金凯3号在陇东旱塬露地种植的适宜密度为60 000株/hm2,在此密度下和中等水肥条件下,综合性状表现优良,产量可达5 612.2 kg/hm2.