美丽针葵耐寒性试验

美丽针葵耐寒试验研究结果表明 :未经低温锻炼的叶片相对电导率在冷冻处理 4～ 6h时急剧增加 ,而经低温锻炼的叶片相对电导率急剧增加时间为 8～ 12h之间 ;在冷冻处理情况下 ,经低温锻炼的美丽针葵叶片丙二醛含量明显低于未经低温锻炼植株 ,而低温保护性物质脯氨酸的含量明显高于未经低温锻炼的叶片 ,显示经低温锻炼的美丽针葵抗寒能力明显好于未经低温锻炼的植株     

基于Cap-cyclide坐标的非圆截面等离子体的稳定性研究

通过导人Cap-cyclide坐标，进行了导体壁对非圆截面等离子体稳定性影响的研究，得出了等离子体的稳定性随着导体壁接近等离子体的表面而增加这一结论。     

鸡血浆中环丙沙星的RP-HPLC法研究

采用反相高效液相色谱(RP-HPLC)法,以氧氟沙星为内标物,测定鸡血浆中环丙沙星的含量。结果表明:环丙沙星回收率为88.20%～95.41%,日内、日间相对标准差分别为3.22%～7.04%、5.05%～9.00%(n=5),环丙沙星在0.05～7.50mg·L-1范围内呈显著线性相关(r为0.9993,n=9),最低检测限为0.015mg·L-1,可满足环丙沙星在肉仔鸡体内的药动学样品检测的要求。     

高效液相色谱法测定鸡体内的替硝唑

建立了鸡血浆和肌肉中替硝唑 (Tinidazole,TNZ)的高效液相色谱 (HPL C)测定法。采用 Shim adzu L C- 6 A型高效液相色谱系统 ,YWG- C1 8柱 ,UV检测器 ,以甲醇 /水 (32∶ 6 8)为流动相 ,流速 0 .8m L/ m in,波长 318nm,室温测定。用二氯甲烷萃取血浆和肌肉中 TNZ。结果表明 ,TNZ的最低检出限为 0 .0 12 5 mg/ L,在血浆和肌肉中的最低定量限分别为 0 .0 2 5 mg/ L和 0 .0 2 5μg/ g。其质量浓度在 0 .0 2 5～ 6 4mg/ L范围内与色谱峰高呈良好线性关系 (r=0 .9999)。TNZ质量浓度为 0 .0 2 5、1.6、6 4mg/ L 时 ,日内相对标准差小于 5 % ,日间相对标准差小于 10 % ,在血浆和肌肉中的回收率分别为 98.0 0 %、91.88%、96 .17%和 90 .0 0 %、99.0 6 %、92 .5 4%。该方法灵敏、快速、简便 ,适用于 TNZ在鸡体内的药物动力学和组织中残留研究     

The Influence of Selection for Plasma very Low Density Lipoprotein Concentration upon Production Performance in Layer Type Chickens

The chickens studied were from a pure line of brown shell egg-type(YAFA) female grandparents of 17-week-old.Plasima very low denstiy lipoprotein(VLDL) concentration was measured with turbidimetric assay.The experiment results showed that the phenotypic correla-tions between 29-week or 50-week plasma VLDL concentration and egg production (EP) were positive in the early stage of laying period,but those in the latter stage were negative,Selection for low plasma VLD concentration will decrease the EP in the early stage of laying period but in-crease the one in the latter stage,There was a significant negative phenotypic correlaiton between the atge at first egg (AFE) and 18-week body weight(BW),The phenotypic correlation between 29-week plasma VLDL concentration and the AFE was negative,AFE was influenced by BW and body fatness as well.There was significant positive phenotypic correlation between plasima VLDL concentration and body weights(BWs) at the same stage in laying period and the phenotypic correlation was aslo positive between 29-week plasma VLDL concentration and the BW at middle stage of laying period,indicating that selection for low plasma VLDL concentration would reduce BWs at various stages to different degree.     

镉对金鱼性激素的分泌及某些生理生化效应的研究

镉浓度40（mg/l）染毒金鱼10、20、35天的实验结果表明：镉引起了染毒鱼的雌二醇和睾酮增高。甲状腺激素（T3）含量仅在染毒10天时增高（P＜0．05），尔后变化不明显。此外，染毒鱼和血浆离子Mg^2 浓度和血糖均升高（20天），其他离子K^ 、Na^ 无明显变化。测试生长指标表明：此剂量镉对金鱼的生长在染毒20天时无抑制作用。染毒35天时，染毒鱼倾向于负生长。     

精清对蓝狐精子常温保存存活时间的影响

用手按摩法采集蓝狐精子和精清,并用同源精清和稀释液分别在不同稀释倍数下稀释同一份精液,测出此种条件下蓝狐精子的存活时间。统计分析表明,精清与稀释液处理的蓝狐精液差异显著(P <0 .0 5 ) ,精清不利于蓝狐精子的体外常温保存     

半乳甘露寡糖对猪门静脉血流速率、氨基酸和葡萄糖的净吸收量及耗氧量的影响

通过对10头15kg体重试验猪实施门静脉血管插管等外科手术,并结合回肠肠系膜静脉灌注对氨基马尿酸(PAH)技术,探讨半乳甘露寡糖对生长猪门静脉血浆流率(PVPF)和血液流率(PVBF)、氨基酸和葡萄糖的净吸收量及耗氧量的影响。10头杜长大阉公猪随机分为2个处理,每个处理5头,单笼饲养于可调节不锈钢代谢笼内,经过14d适应期后,在门静脉、肠系膜静脉、颈动脉安装插管,开始15d正式试验。对照组饲喂玉米-豆粕型基础饲粮,试验组饲喂基础饲粮 0.20%半乳甘露寡糖。结果表明,经过15d饲喂期后,与对照组相比,饲粮添加半乳甘露寡糖可显著降低试猪食后8h内门静脉的平均血浆流率和血液流率,显著提高食后8h内门静脉对氨基酸和葡萄糖的净吸收量,显著降低食后8h内门静脉的耗氧量,也就是说半乳甘露寡糖可通过减少生长猪小肠黏膜对氨基酸和葡萄糖的氧化而增加肠外组织对其吸收。     

皖系粗毛兔血浆酶活性及其与产毛量相关的研究

对49只1岁龄皖系粗毛兔进行产毛量和血浆中碱性磷酸酶、谷丙转氨酶、谷草转氨酶活性的测定.结果表明,皖系粗毛兔1岁时血浆碱性磷酸酶活性与产毛量之间呈显著的正相关(P＜0.05);血浆中谷丙转氨酶和谷草转氨酶活性与产毛量之间的相关均不显著(P＞0.05).     

Relationship between hydration estimate and body weight change after fluid therapy in critically ill dogs and cats

Objective: To characterize the relationship between clinical estimates of hydration in dogs and cats admitted to an intensive care unit (ICU) and changes in their body weight following 24–48 hours of fluid therapy. Design: Outcome study. Setting: ICU at a veterinary teaching hospital (VTH). Animals: A total of 151 dogs and 42 cats with various medical disorders that had not had surgery within 48 hours of admission into the ICU were consecutively admitted into the study. Animals with any condition predisposing to excess fluid loss or retention were excluded: heart disease, sepsis, trauma, pancreatitis, pleural or pericardial effusion, ascites, and pathologic oliguria. Animals that acquired any of the following during the observation period were excluded: gastrointestinal fluid loss, edema or diseases predisposing to edema, oliguria, diuretic therapy, and body fluid drainage or hemorrhage. Fluid therapy was ordered based on estimate of hydration at admission. Other treatments were not modified or withheld. Interventions: Physiologic data were collected at the time of admission and 24–48 hours later. Measurements and main results: Hydration was estimated on admission to the ICU using clinical judgement with no supporting laboratory data. Each admitting clinician used this estimate to plan fluid therapy. Fluid therapy was defined as the administration of any enteral or parenteral fluids as well as any decision to withhold fluids. Paired measurements taken on admission and at 24–48 hours included packed cell volume (PCV), total plasma solids (TS), and body weight. Amount and type of fluids or blood products administered were noted. Neither clinician estimates of dehydration nor baseline PCV or TS predicted clinically significant changes in body weight following fluid therapy, and there was no relationship between weight change and changes in PCV or TS. Conclusions: A clinical diagnosis of dehydration in our ICU does not predict weight gain following fluid therapy. Neither baseline PCV/TS nor changes in these measurements following 24–48 hours of fluid therapy predicted changes in body weight.