甘蔗黄叶病毒的TBIA快速检测

利用从法国引进的甘蔗黄叶病毒（SCYLV）标准抗原抗体，研究建立了TBIA快速检测SCYLV的方法。通过对田间采集的样本进行检测，同时以DAS—ELISA检测法印证，检测结果一致，表明TBIA能简便、快速、准确、有效地检测出SCYLV，且检测效率高，适合于田间大批量样品快速检测。为甘蔗黄叶病的诊断和防治、脱毒种苗的生产、对外甘蔗品种／材料交换检疫检测提供了技术支撑。     

聚类分析在管网故障点定位中的应用

在管网状态估计的基础上,对管网进行故障(泄漏)模拟,建立管网故障模拟数据库.利用多元统计学上的聚类分析方法,对管网故障模拟的数据进行聚类分析,将需要研究的管网的所有管段虚拟地分为M个区域,即"虚拟分区".并利用多元统计学上的判别分析方法,对泄漏情况进行故障定位.     

Dot-ELISA检查香肠中沙门氏菌的研究

以硝酸纤维膜为固相载体,利用沙门氏菌多价血清和辣根过氧化物酶(HRP)标记制备的羊抗兔IgG,成功地建立对香肠的Dot-ELISA检查法,同时,采用常规分离培养鉴定技术作对照试验。结果显示,在86份香肠中,用Dot-ELISA检出沙门氏菌阳性为36份,阳性率为41.86%;而常规分离培养鉴定技术检出沙门氏菌阳性为34份,阳性率为39.53%,两种方法的阳性符合率为86.17%,经统计分析,t=0.311 1,p>0.05,两种方法差异不显著。     

国内蔓性长豇豆系列品种的抽测与综合评价

通过田间试验对国内31个蔓性长豇豆品种进行重复鉴定。结果表明,约有四分之一的品种质量不合格;约有十分之九的品种不早熟,产量与对照之豇28-2差异不显著,约有二分之一的品种抗病性比对照差。国内虽有众多品种育成,但由于遗传性状的局限性而不能取代老品种之豇28-2。长豇豆虽然是高度自花授粉作物,但忽视选择也极易混杂退化。黑子类品种和柱头外露品种在留种繁殖,更要注意品种间隔离,并要多次进行去杂去劣。     

猪圆环病毒Ⅱ型PCR检测方法的研究

【目的】建立快速、简便、特异的检测猪圆环病毒II型的PCR方法;【方法】根据已发表的猪圆环病毒II型基因序列设计合成了一对引物,用酚-氯仿法抽提可疑病料中的病毒DNA,应用PCR技术对猪圆环病毒进行基因扩增,PCR产物进行序列测定;以猪瘟病毒、猪伪狂犬病毒、猪细小病毒为对照,进行特异性试验;取部分病料进行重复性试验;【结果】PCR方法扩增出了长度为702bp的片段,扩增产物经测序和序列分析表明扩增的序列为PCV2序列;猪瘟病毒、猪伪狂犬病毒、猪细小病毒的PCR扩增均为阴性,与预期结果一致;部分病料PCR重复检测5次,检测结果完全一致;【结论】PCR检测方法可以对猪圆环病毒病作出敏感、特异、准确地诊断,该方法检测的基因最低模板量为2×10-5ng/ml。     

Influence of MUD on the System Capability of CDMA

In CDMA communication system, the relativities between users result in Multiple Access Interference (MAI). With the increase of users, MAI becomes the main jam of broadband CDMA communication system. Multi-User Detection (MUD) is the most important technology of anti-jamming in the broadband CDMA communication system, which can eliminates MAI effectively by using the information of all user signals to detection single. This paper analyze the expression of system capability without MUD and with MUD, and puts forward the way to increase system capability by using MUD. Then the influence of MUD on CDMA system capability is discussed by MATLAB emulation. Basing analysis and emulation, the conclusion is got: the higher the MUD efficiency, the better the improvement of CDMA system capability; under the same MUD efficiency, the lower data rate, the smaller inter-cell interference, the lower the ratio of bit energy to power spectrum, the better the improvement of CDMA system capability.     

分子印迹技术在兽用抗生素残留分析中的应用进展

随着规模化养殖业发展,兽用抗生素大量使用,不合理甚至违法添加使用导致的残留现象已成为威胁动物源食品安全的重要因素,迫切需要建立灵敏可靠的残留分析方法。从复杂的生物样品中提取纯化微量的残留药物是制约兽药残留检测方法建立的关键。分子印迹技术具有特异性强、灵敏度高、操作简便等优点,在兽药残留检测研究中得到越来越多重视。文章主要综述了分子印迹聚合物的最新制备方法及在兽用抗生素残留分析中的最新研究进展。     

兽药中非法添加物检测及风险防范技术研究

本文总结了近年来兽药中非法添加的规律,分析成因;全面梳理现有检测标准,对非法添加物检测方法的检测对象和目标药物进行统计分析,研究检测方法发展及内在联系。在非法添加物检测方法提升和风险防范方面进行思考,提出筛查与确证方法并重、进一步扩大检测方法适用性、加强高效、高通量检测技术研究、拓展非法添加物检测品种范围;加强检查与宣传、疏堵结合,全面减少非法添加风险。     

Detection of fluoroquinolone resistance level in clinical canine and feline Escherichia coli pathogens using rapid real-time PCR assay

Fluoroquinolones are used to treat infections caused by Escherichia coli in canine and feline veterinary patients, particularly those infecting the urinary tract. The gyrA gene is a primary target causing fluoroquinolone resistance in Gram negative coliforms, with mutations in codons 83 and 87 generally associated with high-level of resistance E. coli clinical isolates. We have developed a fluorescence resonance energy transfer (FRET) quantitative PCR to identify enrofloxacin-resistance in clinical E. coli isolates that carry mutations in codons 83 and 87 of gyrA. This real-time quantitative PCR assay is rapid, economical, and sensitive compared with cultured antimicrobial susceptibility testing. The assay identified as few as four genome copies per reaction from culture and 19 genome copies in urine. For the 70 isolates tested, the sensitivity was 87.5% (95% CI = 75–95.3%) (n = 42/48), specificity was 100% (95% CI = 87.3–100%) (n = 22/22), whereas accuracy was 91.4% (95% CI = 82.3–97%) (n = 64/70). Furthermore, we were able to accurately differentiate between the wild type and mutants E. coli directly from infected canine urine samples (n = 5) within 2 h. These results were confirmed by sequence alignments of the PCR products and comparison with the susceptibility testing. The FRET-PCR assay appears to have promising clinical application as an early diagnostic tool for rapid and sensitive detection and differentiation of the level of fluoroquinolone resistance among clinical E. coli isolates that may facilitate design of the dosing regimen.     

大豆炸荚表型精准检测方法的研究

大豆炸荚表型易受环境影响,炸荚相关研究的关键在于如何获得精准的表型。以遗传背景较近且炸荚表型差异较大的2份栽培大豆为材料,利用烘箱对2个品种完熟期不同部位的豆荚进行炸荚率检测,烘干温度和持续时间各设置5个处理。结果表明,不同处理下大豆各部位的炸荚率无显著差异,炸荚率与烘干温度及持续时间呈显著正相关;不同品种大豆炸荚率在60℃、11h后的处理均存在显著性差异,综合炸荚检测的便利、安全及种子发芽率的影响,最终确定烘干温度60℃、持续时间11~13h为最佳检测条件。