一例羊肝片吸虫病的诊治

肝片吸虫病又叫肝蛭病，是由肝片形吸虫寄生在羊肝脏胆管引起的一种蠕虫病，以破坏动物肝脏、胆管，引起急性、慢性肝炎、胆管炎为特征。多呈地方性流行，是当前严重危害养羊业的内寄生虫病。     

腓骨闭合复位弹性钉内固定联合胫骨其它固定方式治疗胫腓骨骨折

目的:观察腓骨闭合复位弹性钉内固定联合胫骨其它固定方式治疗胫腓骨骨折的临床效果。方法:回顾性分析医院2013年3月至2015年3月接诊的胫腓骨骨折患者99例,其中男53例,女46例,年龄25~60岁。随机分为研究组与对照组,研究组患者51例,采用腓骨闭合复位弹性钉内固定联合胫骨其它固定方式治疗胫腓骨骨折,对照组患者48例采用腓骨切开复位内固定联合胫骨其它固定方式治疗胫腓骨骨折,对比两组患者的治疗有效率、术后并发症。结果:所有患者经手术治疗后,均得到了一定的临床效果,研究组患者的有效率为98.04%(50/51),术后并发症的发生率为9.80%(5/51);对照组患者的治疗有效率为87.50%(42/48),术后并发症的发生率为31.25%(15/48),研究组患者的临床效果明显优于对照组患者(P0.05)。结论:腓骨闭合复位弹性钉内固定联合胫骨其它固定方式治疗胫腓骨骨折可以取得较好的临床效果,对患者的创伤小,固定牢靠,并发症少。     

小芝麻告诉你——肝脏的杀手

1．乙肝病毒，提起它来真可谓无人不知，无人不晓啊，因为乙肝病毒的足迹遍布大江南北，世界各地。 2．据调查，我同人群乙肝的感染率约为60％，即有7亿多人感染过乙肝病毒。乙肝病毒侵入人体，最终使人忠上慢性肝炎、肝硬化和原发性肝痛。¨前，全世界对乙肝尚无特效治疗药物。     

Study on Developmental Expression of ApoCⅡ Gene mRNA in Liver Tissues of Pigs

The aim of this study was to investigate the developmental patterns of ApoCⅡ gene mRNA in liver in Mashen and Large White pigs, and study the relationship between the expression level of ApoCⅡ and the lipid metabolism in pigs.The mRNA relative expressions of ApoCⅡ gene in liver at seven stages of 1,30,60,90,120,150,and 180-day old in Mashen and Large White pigs were determined by quantitative Real-time PCR.The results showed that the developmental trend of ApoCⅡ mRNA expression in liver between Mashen and Large White pigs was different.The ApoCⅡ mRNA abundance was decreased from birth to 60-day old,then increased at 90-day old,and decreased again after that in Mashen pig.However,the relative expression amount in Large White pig was gradually decreased from birth to 150-day old and increased again at 180-day old.Except for the ApoCⅡ mRNA expression amount at 1-day old,the differences of the expression amount at other stages in Mashen and Large White pigs were significant or extremely significant (P<0.05 or P<0.01).The ApoCⅡ mRNA expression in liver was affected by age and breed,and could play an important role in lipid metabolism in pigs.     

高铜日粮对肉鸡肝脏Trx2蛋白表达的影响

将200羽1日龄Cobb商品代肉鸡随机分为4组,各组日粮中铜含量分别为:对照组(Ⅰ组)11 mg/kg、试验Ⅱ组110 mg/kg、试验Ⅲ组330 mg/kg、试验Ⅳ组550 mg/kg。饲养至10、30、50 d时每组各取5只鸡用于采集肝脏样品,提取肝脏总RNA用RT-PCR法测定Trx2基因m RNA在肝脏中的表达量,用蛋白质印迹法检测Trx2蛋白表达量。结果显示:日粮铜含量为11 mg/kg、110 mg/kg和330 mg/kg时,随着试验时间的延长,肝脏Trx2基因表达升高,但差异不显著(P0.05),而铜含量为550 mg/kg时,Trx2基因表达量降低且50 d时差异显著(P0.05);随着试验时间的延长,各试验组的Trx2蛋白表达量均升高,且330 mg/kg和550 mg/kg组在50 d时差异显著(P0.05)。试验表明,饲喂高铜日粮可导致线粒体型硫氧还蛋白2(Trx2)mRNA在肝脏中的表达量先升高后降低,蛋白表达量升高。     

社区弹性景观规划与设计的探讨

社区不仅是供给人类长居的环境,还是人类应对飓风、地震以及暴乱等多种多样风险的避难所。基于此,越来越多的国家开始提倡建设弹性社区,以此来提高社区的抗风险能力。文章即围绕社区弹性景观的规划与设计展开探讨,从景观学角度为弹性社区的创建提供可行性方法。     

网络购物狂欢节背后的经济现象分析

针对当今社会"网购"热现象的产生及影响从经济学角度进行了分析、解刨。同时,对参与"网购"的买方、卖方及相关各群体提出了相应的建议和对策。     

代谢组学在反刍动物营养研究中的应用进展

代谢组学是研究生物系统中所有代谢物的一门新兴学科。它能够检测生命系统中某一特定生物层次的所有代谢物的变化,并能够利用其特有的分析技术平台和数据分析平台对所得代谢物进行系统性度量。近年来,因其测试技术的广泛适用性,代谢组学逐渐被应用于反刍动物的营养学研究中。本文从瘤胃代谢组学、肝脏代谢组学、乳腺代谢组学和血液代谢组学等4个方面对代谢组学在反刍动物营养研究中的应用进展进行了综述。     

Study on Liver-protective Effect of Chinese Herbal Compound Probiotics on Acute Liver Injury Layers

This experiment was conducted to investigate the liver-protective effect of Chinese herbal compound probiotics (CHCP) on acute liver injury layers.One hundred and eight 1 day old hens were divided into 4 groups with 3 replicates in each group and 9 layers per replicate in trial 1.The layers in model groups Ⅰ to Ⅲ were gavaged with 10% (V/V) soybean oil solution of carbon tetrachloride (SCCl₄) according to 1,2 and 4 mL/(kg·BW) at 14,28 and 35 d,respectively.The layers in control group were gavaged with 2 mL/(kg·BW) soybean oil.In trial 2,sixty 1 d layers were divided into 5 groups:Control group (soybean oil),model control group(SCCl₄)and low-dose,middle-dose and high-dose CHCP group (SCCl₄+1‰ CHCP,SCCl₄+2‰ CHCP and SCCl₄+4‰ CHCP respectively).CHCP were used by drinking water since 7 days.SCCl₄ were gavaged according to 2 mL/(kg·BW) at 14 and 28 d.The results showed as follows:The model of layers liver damage could be built by intragastric administration of 2 mL/(kg·BW) 10%(V/V) SCCl₄ at 14,28 d respectively,with the signs of hepatic steatosis,severe vacuolar degeneration,nuclear condensation and necrosis.Compared to the model control group,the serum AST levels in low,medium and high dose CHCP groups were decreased by 4.35% (P > 0.05),7.57% (P > 0.05) and 9.79% (P < 0.05),the serum ALT levels in medium and high dose CHCP groups were decreased by 34.92% (P < 0.01),36.51% (P < 0.01),the serum total bilirubin content in medium and high dose CHCP groups were decreased by 25.49% (P < 0.01),27.45% (P < 0.01).The liver cell congestion was reduced to varying degrees in different dose CHCP groups,and the liver cell had no vacuoles,arranged in neat rows,abundant cytoplasm and uniform in different dose CHCP groups.In conclusion,2‰,4‰ CHCP could reduce hepatocyte necrosis,decrease the serum activities of ALT,AST and total bilirubin levels,and had protective effect on hepatic injury induced by SCCl₄.