太子河流域参考作物腾发量演变特征及气候影响因素分析

采用太子河流域内8个气象站1960～2005年间气象资料，应用Penman-Montieth公式计算了46年间逐月参考作物腾发量(ET₀)，对参考作物腾发量及气象要素的年际变化特征、月际变化特征及趋势进行了分析，应用统计检验方法分析了影响流域参考作物腾发量变化的主要气象因素。结果表明：近46年间太子河流域ET₀值呈现缓慢下降趋势，年内ET₀值分布以5、6月份最高，1月份最低。影响ET₀的主要气候要素按影响程度强弱依次为日照、风速、温度、相对湿度。     

Establishment of a proteomic reference map for the gastrocnemius muscle in the rabbit (Oryctolagus cuniculus)

In several laboratory and production species, the establishment of a proteome reference map of a specific tissue has been accomplished. The rabbit is widely used as both a production and experimental animal. A lot of physiology research involving the gastrocnemius muscle of rabbit is described, although no reference proteome map is available. In this work, the first reference map of the rabbit’s gastrocnemius muscle using 2D gel electrophoresis and the identification of proteins through peptide mass fingerprinting (PMF) was established. A total of 45 proteins were localized and identified with three major roles: cell structure and contractile apparatus; metabolic and cell defense proteins. A reference map of major proteins expressed is described enabling possible comparisons with other physiological studies.     

Estimation of reference intervals from small samples: an example using canine plasma creatinine

Background: According to international recommendations, reference intervals should be determined from at least 120 reference individuals, which often are impossible to achieve in veterinary clinical pathology, especially for wild animals. When only a small number of reference subjects is available, the possible bias cannot be known and the normality of the distribution cannot be evaluated. A comparison of reference intervals estimated by different methods could be helpful. Objective: The purpose of this study was to compare reference limits determined from a large set of canine plasma creatinine reference values, and large subsets of this data, with estimates obtained from small samples selected randomly. Methods: Twenty sets each of 120 and 27 samples were randomly selected from a set of 1439 plasma creatinine results obtained from healthy dogs in another study. Reference intervals for the whole sample and for the large samples were determined by a nonparametric method. The estimated reference limits for the small samples were minimum and maximum, mean ± 2 SD of native and Box–Cox‐transformed values, 2.5th and 97.5th percentiles by a robust method on native and Box–Cox‐transformed values, and estimates from diagrams of cumulative distribution functions. Results: The whole sample had a heavily skewed distribution, which approached Gaussian after Box–Cox transformation. The reference limits estimated from small samples were highly variable. The closest estimates to the 1439‐result reference interval for 27‐result subsamples were obtained by both parametric and robust methods after Box–Cox transformation but were grossly erroneous in some cases. Conclusion: For small samples, it is recommended that all values be reported graphically in a dot plot or histogram and that estimates of the reference limits be compared using different methods.     

基于极限学习机的参考作物蒸散量预测模型

为实现气象资料缺乏情况下参考作物蒸散量（reference crop evapotranspiration, ET0）高精度预测,以气象因子的不同组合为输入参数,利用FAO-56 Penman-Monteith公式计算的ET0作为预测标准值建立基于极限学习机（extreme learning machine, ELM）的ET0预测模型。选取川中丘陵区7个气象站点1963－2012年逐日气象资料进行模型训练与测试,并将模拟结果同Hargreaves、Priestley-Taylor、Makkink及Irmark-Allen等4种常用模型进行对比。结果表明：ELM模型能很好地反映气象因子同ET0间复杂的非线性关系,且模拟精度较高;基于最高和最低温度的ELM模型模拟精度（均方根误差和模型效率系数分别为0.504 mm/d和0.827）高于Hargreaves模型（均方根误差和模型有效系数分别为0.692 mm/d和0.741）;基于最高、最低温度和辐射的ELM模型模拟精度（均方根误差和模型有效系数分别为0.291 mm/d和0.938）明显高于Priestley-Taylor（均方根误差和模型有效系数分别为0.467 mm/d和0.823）、Makkink（均方根误差和模型有效系数分别为0.540 mm/d和0.800）和Irmark-Allen模型（均方根误差和模型有效系数分别为0.880 mm/d和0.623）。因此基于最高、最低温度和辐射的ELM模型可以作为气象资料缺乏情况下川中丘陵区ET0计算的推荐模型。该研究可为川中丘陵区气象资料缺乏情境下ET0精确计算提供科学依据。     

用^137Cs法探讨苏南坡地的土壤侵蚀

与黄土和红壤地区相比 ,学术界对苏南丘陵区土壤侵蚀的关注要少得多 ,也许是这里的土壤侵蚀的绝对数量不大 ,然而 ,苏南地区的土壤侵蚀引起的生态影响却不容忽视 ,如因土壤侵蚀造成的养分流失对水体的影响及土层减薄都远超过黄土区。为了探讨苏南土壤侵蚀的生态影响 ,首先是要了解土壤侵蚀的状况 ,在这方面 13 7Cs示踪法是一种非常有用的工具 ,可是要使用此法 ,必须要解决两个问题 ,一是确定研究区的背景值 ,二是建立估算土壤侵蚀的模型。经过较长时间的研究 ,确定苏南地区的 13 7Cs背景值为 2 2 0 0 Bq/m2 ;并根据基本物理含义 ,建立了耕地与非耕地的土壤侵蚀模型 ,用这 2个模型并借用其他学者提供的土壤沉积模型 ,对宜兴竹园及茶园两个坡地的土壤侵蚀与沉积作了估算。在此基础上 ,又尝试着简单探讨了土壤侵蚀引起的土层及养分流失的生态影响     

发生分类淋溶土与系统分类参比特征研究

利用最新建立的中国 1∶10 0万土壤数据库 ,研究了我国发生分类淋溶土与中国土壤系统分类的参比及其在中国土壤系统分类下的空间分布和数量特征。结果表明 ,我国发生分类淋溶土总面积为10 5 975 7.8km2 ,分布规律明显 ;与系统分类参比 ,发生分类淋溶土分属于中国土壤系统分类 4个土纲 ,即淋溶土、雏形土、新成土和灰土 ,它们分别占发生分类淋溶土总面积的 72 .8%、2 6 .1%、1.0 %、0 .1% ,其中包含系统分类的 2 5个土类和 4 7个亚类 ,参比关系复杂 ,不是简单的一对一的关系。分析发生分类某一类型土壤分属于系统分类不同类型的面积比例及其标准偏差 ,结果表明土壤参比的单元级别越低 ,越易于参比和把握。为了使中国土壤系统分类更实用、更易于普及 ,深入开展土壤基层分类研究 ,进一步发展和完善中国土壤系统分类是必要的 ,也是十分迫切的。研究结果对于土壤类型的正确参比、中国土壤系统分类的应用与发展 ,具有很好的参考应用价值     

基于非洲猪瘟病毒标准物质的荧光PCR检测试剂盒初步评价

为探究非洲猪瘟病毒(African swine fever virus,ASFV)标准物质作为试剂盒评价体系的可行性,比较了市场上5种主流品牌ASFV荧光PCR检测试剂盒的检测性能。使用ASFV P72基因核酸标准物质作为模板,根据5种试剂盒说明书分别进行相应的荧光定量PCR检测,结合扩增曲线、Ct值,分析不同试剂盒的敏感性、可重复性以及所需反应时间。结果显示:5种试剂盒的阴性、阳性对照均成立,最低检测限均为5.9×10^-1拷贝/μL;4个厂家的试剂盒线性关系R2>0.98,其中最优的R²=0.994,离散度最小;各试剂盒的实际反应耗时与理论反应耗时均有一定差异(0.20~0.96 h)。结果表明,各生产厂家使用P72基因作为靶基因研制的ASFV荧光PCR检测试剂盒都可以使用ASFV标准物质作为评价体系,来评判试剂盒的检测性能。本试验为各实验室不同样品检测的ASFV荧光PCR检测试剂盒选择提供了一种可用的评价方法。     

个人文献管理软件在科研工作中的应用

介绍了NoteExpress文献管理软件在个人科研工作分项管理方面的强大功能,以期广大科研工作者能够灵活运用NoteExpress管理个人的电子文献,提高文献管理和利用的效率,并能将更多的精力投入到研究课题中去。这对科研工作者提高科研和论文工作效率有着非常重要的实用意义。     

Prediction of daily reference evapotranspiration by a multiple regression method based on weather forecast data

Prediction of daily reference evapotranspiration (ET ₀) is the basis of real-time irrigation scheduling. A multiple regression method for ET ₀ prediction based on its seasonal variation pattern and public weather forecast data was presented for application in East China. The forecasted maximum temperature (T _max), minimum temperature (T _min) and weather condition index (WCI) were adopted to calculate the correction coefficient by multilinear regression under five time-division regimes (10 days, monthly, seasonal, semi-annual and annual). The multiple regression method was tested for its feasibility for ET ₀ prediction using forecasted weather data as the input, and the monthly regime was selected as the most suitable. Average absolute error (AAE) and root mean square error (RMSE) were 0.395 and 0.522 mm d^?1, respectively. ET ₀ prediction errors increased linearly with the increase in temperature prediction error. A temperature error within 3 K is likely to result in acceptable ET ₀ predictions, with AAE and average absolute relative error (AARE) <0.142 mm d^?1 and 5.8%, respectively. However, one rank error in WCI results in a much larger error in ET ₀ prediction due to the high sensitivity of the correction coefficient to WCI and the large relative error in WCI caused by one rank deviation. Improving the accuracy of weather forecasts, especially for WCI prediction, is helpful in obtaining better estimations of ET ₀ based on public weather data.     

渔业管理中生物学参考点的理论及其应用

生物学参考点常被表示为与渔业管理相关联的捕捞死亡率和生物量,是单从生物学角度来衡量渔业资源及其开发状况的指标。通常可分为目标参考点、限制参考点和阈值参考点。目标参考点是为了持续获得某一目标渔获量所需的最小生物量和相应捕捞死亡率,包括目标生物量(Bmsy)、目标产卵亲体量(Smsy、SSB35%、SSB40%)、目标捕捞死亡率(Fmsy、Fmax、F0.1、Fmed、F40%、F40%)等参数。限制参考点用于保证捕捞死亡率不会高到危害鱼类种群的可持续利用和其生物量不会低到危害其生存,主要包括Fmsy、Fmax、F0.1、Fcrash、F20%、Bloss等参数。阈值参考点介于目标参考点和限制参考点之间,包括预防性捕捞死亡率Fpa、预防性生物量Bpa,主要对渔业资源的开发和管理进行预警,防止生物量小于BL。生物学参考点主要应用动态综合模型、产量模型和亲体量补充量关系模型来估算,估算过程中需要考虑到补充、生长、死亡等生命史过程中的不确定性。本文对生物学参考点的发展和应用进行了综述,并以金枪鱼渔业为例阐述它在渔业管理上的应用。近几十年来,我国近海渔业资源出现衰退,亟需利用生物学参考点的原理和方法对重要渔业资源种类进行...