排序方式: 共有67条查询结果,搜索用时 109 毫秒
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WANG Huan-yu WANG Ya-feng ZHANG Kun-song ZHANG Chao-hui ZHANG Zi-jian HUANG Shan-zhou WU Jian PENG Bao-gang CHEN Dong ZHOU Qi 《园艺学报》2015,31(6):1019-1025
AIM: To investigate the effects of microRNA145 (miRNA145) on the viability, apoptosis, invasion and metastasis of hepatoma HepG2 cells. METHODS: HepG2 cells were randomly allocated into 3 groups: blank control group, empty mimic transfected group and miRNA145 mimic transfected group. Under the induction of LipofectamineTM 2000, the recombinant was transfected into HepG2 cells. After transfection, the expression level of miRNA145 was detected by real-time PCR. The protein level of N-cadherin and the mRNA expression levels of miRNA145 and N-cadherin were detected by Western blot and real-time PCR. The cell viability was detected by MTS assay. The cell cycle and apoptosis were analyzed by flow cytometry. Invasion and metastasis were detected by Transwell assay. RESULTS: Compared with negative control, miRNA145 expression was up-regulated significantly, while the expression of N-cadherin was down-regulated significantly. Meanwhile, the cell viability, cell cycle, apoptosis, invasion and metastasis of hepatoma HepG2 cells were all significantly inhibited (P<0.05). CONCLUSION: miRNA145 dramatically inhibits viability, apoptosis, invasion and metastasis of hepatoma cells. 相似文献
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农杆菌介导microRNA398靶基因转化大豆 总被引:1,自引:0,他引:1
拟南芥miR398对低磷胁迫有响应。通过生物信息学方法预测找到大豆miR398a的靶基因铜/锌超氧化物歧化酶GmSODC(Copper/zinc superoxide dismutase),通过RT-PCR扩增得到GmSODC全长cDNA,采用LIC(Ligation-Independent cloning)法将GmSODC连接到双元植物表达载体pJG045上,构建成植物表达载体pSDC6。通过农杆菌介导的子叶节法将GmSODC基因导入大豆品种东农50中,获得了转化GmSODC的T1代植株。采用Real-time PCR的方法,对转基因植株进行GmSODC基因转录水平的相对定量分析,其中1株较非转基因植株表达量明显提高。 相似文献
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[目的]探索氧化型低密度脂蛋白受体OLR13′UTR A223C多态与奶牛乳脂率高低紧密关联的分子机制。[方法]用miRanda(1.0)软件装载牛microRNA数据库,预测OLR13′UTR潜在的microRNA。[结果]共预测16个microRNA靶标,OLR13′UTRA223C突变定位于bta-miR-370靶标种子序列的互补区。[结论]由于OLR13′UTRA→C突变导致bta-miR-370靶标的消失,为OLR13′UTR A223C多态与奶牛乳脂率高低紧密关联的分子机制深入研究提供依据。 相似文献
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AIM To investigate the effect of microRNA let-7a on neuronal apoptosis in a rat model of intracerebral hemorrhage (ICH). METHODS Forty-eight healthy SD rats were selected, 8 of which served as sham group, and 2 μL of type VII collagenase was injected into globus pallidus of the remaining rats to construct ICH model. These ICH rats were randomly divided into model (2 μL normal saline) group, let-7a agomir group, NC agomir group, let-7a antagomir group and NC antagomir group, with 8 rats in each group, and the corresponding drugs were injected into the lateral ventricle. After 7 d, the neurological function scoring was performed, and HE staining was used to observe the pathological damage. RT-qPCR and Western blot were used to detect the expression of related proteins. TUNEL staining was used to detect neuronal apoptosis. The targeting relationship between let-7a and mitogen-activated protein kinase kinase kinase kinase 3 (MAP4K3) was predicted by bioinfomatic software, and further verified by dual-luciferase reporter assay. RESULTS In animal experiments, when let-7a was over-expressed, neurological function scores were reduced,and the pathological damage was alleviated. Glial fibrillary acidic protein (GFAP) expression was down-regulated, neuronal apoptosis was reduced, and the protein levels of cleaved caspase-3 and cleaved PARP were decreased (P <0.05). In cell assays, MAP4K3 was one of the target genes of let-7a, and MAP4K3 was negatively regulated by let-7a. let-7a over-expression inhibited the expression of MAP4K3/MKK4/JNK. CONCLUSION let-7a reduces neuronal apoptosis in ICH rats by inhibiting the MAP4K3/MKK4/JNK signaling pathway. 相似文献
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Atherosclerosis is one of the cardiovascular diseases that seriously endanger human health. The pathogenesis of atherosclerosis is very complicated and related to a variety of risk factors. In recent years, many studies have found that circular RNAs play an important regulatory role in the occurrence and development of atherosclerosis by inducing proliferation, differentiation, migration and apoptosis of vascular smooth muscle cells, endothelial cells and macrophages. This article reviews the basic functions of circular RNAs and its recent research on the regulation of atherosclerosis. 相似文献
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苹果MicroRNA的茎环RT-PCR检测及离体试管苗与田间苗表达差异 总被引:4,自引:0,他引:4
MicroRNA(miRNA)在植物的生长发育过程中起着重要作用。以改进CTAB法提取的总RNA为模板,通过设计茎环反转录引物,利用定性RT-PCR技术从寒富苹果幼嫩叶片、成熟叶片、嫩茎、幼果和根等不同器官中均检测到miRNA,并从寒富苹果叶片中克隆分离出14种miRNA。在此基础上利用半定量RT-PCR技术对寒富苹果离体试管苗与田间苗中10种miRNA的表达差异进行分析,结果发现,miR156和miR157在离体试管苗叶片中表达量明显高于田间苗幼叶。本研究建立了苹果miRNA的茎环RT-PCR检测体系,为研究miRNA在苹果植株生长发育过程中的作用奠定了基础。 相似文献