Inlfuence of FSH Treatment on Expression of CDC25A,TSSK3 and P53in Vitro Cultured Sertoli Cells of Calf

CDC25A, TSSK3 and P53 expressionsin vitro in cultured sertoli cells after FSH treatment were studied in order to provide some data for further researches of spermatogenesis. Different concentrations of...     

玉米霜霉病的侵染途径及防治措施

系统综述了玉米霜霉病的症状表现、发生规律和防治措施等方面的研究进展.加强进境植物检疫、及时清除病残体和选育高抗的玉米品种是防治玉米霜霉病的重中之重.     

Evolution Analysis About Soybean MIR166 Family

MicroRNA(miRNA) is a class of important regulating non-coding small molecular RNA. The gma-MIR166 gene family consists of 21 members and their expression patterns diversify widely. It is important to analyze the evolution of gma-MIR166 gene family in order to understand the evolutionary mechanisms of miRNAs in soybean. In this study, we implemented soybean wide genome block analysis, the molecular phylogeny of gma-MIR166 and block analysis of gma-MIR166 family. The results showed that both chromosome big segmental duplications and tandem duplications were main reasons contributed to the expanding of gmaMIR166 gene family. These findings suggested that gma-MIR166 gene family might originate from one or two ancient miRNA genes. The results of research provided a support for evolutionary study of miRNAs in soybean and related species in Fabaceae.     

Design of Automatic Quasi-synchronizing Device in Rural Small Hydropower Station

Generator synchronization is an important and frequent operation in rural small hydropower station. In order to ensure that generator can be paralleled in the grid automatically, efficiently, and stably in the rural small hydropower station whose parallel in operation still needs to be accomplished manually. This paper described an automatic quasi-synchronizing device, based on 89C52 Single-chip Microcomputer （SCM）. Its hardware includes three modules and its software is structuralized. The device structure is simple, and its reliability is high and the maintenance is easy. Besides, convenient Human Machine Interface （HMI） makes the parameter setting simple and flexible     

Revelation of Rural System of Medical Services in BRICS to China

In recent years, the great achievements of basic medical and healthcare services of BRICS have gained great attention and acknowledgement from the whole world. In their medical system reform, India, Br...     

Design and Experiment of Slave Computer Control System for Applying Variable-rate Liquid Fertilizer

In order to increase the applying rate of liquid fertilizer and reduce environmental pollution, a slave computer control system for applying variable-rate liquid fertilizer was designed. The system used SMC as core processor and electrically controlled pressure regulator as execution component. The characteristic equation of the system was obtained by using classical control theory. Results indicated that the characteristic equation met the requirements of routh-criterion, which indicated the working process of the system was stable. Performance of the slave computer was verified via bench tests. Results demonstrated that there was no significant influence on the response from interclass error. The fertilization error was less than 0.9, and the fertilization accuracy was larger than 97%. The liquid fertilizer emitted by the fertilizing devices had no significant difference in uniformity, which met the demands of the slave computer control system for applying variable-rate liquid fertilizer.     

磁化水在农作物上应用的研究进展

针对磁化水对农业生产的影响,简述了国内外关于磁化水应用的研究动态和发展趋势,介绍了磁化水在促进作物种子萌发、增强抗逆性以及提高产量和改善品质方面的研究进展。     

鸡脂肪组织TCF21基因启动子区DNA甲基化与其表达的关系

旨在研究鸡脂肪组织中TCF21基因启动子区DNA甲基化水平与其表达的关系。以东北农业大学高、低腹脂双向选择品系（简称高、低脂系）第24世代7周龄肉鸡为试验材料，利用RT-qPCR检测高、低脂系肉鸡腹部脂肪组织中TCF21基因的mRNA表达水平；利用生物信息学和双荧光素酶报告系统分析TCF21基因启动子的结构与功能；利用Sequenom MassARRAY飞行质谱检测高、低脂系肉鸡腹部脂肪组织中TCF21基因启动子区CpG位点的甲基化水平；利用CpG甲基转移酶处理TCF21启动子报告基因质粒，分析DNA甲基化对TCF21基因启动子活性的影响。结果显示，高脂系肉鸡腹部脂肪组织中TCF21基因的mRNA表达水平极显著高于低脂系（P<0.001）；TCF21基因的启动子区存在40个CpG位点，且在启动子的近端和远端均有分布，但不存在CpG岛；将TCF21基因的启动子划分为5个功能区域，分别为R1区域（-2 000~-1 500 bp）、R2区域（-1 500~-1 000 bp）、R3区域（-1 000~-500 bp）、R4区域（-500~-200 bp）和Core区域（-200~-100 bp）；高脂系R2、R3和R2+R3区域的DNA甲基化水平显著或极显著高于低脂系（P<0.05或P<0.001）；R2、R3、R2+R3区域的DNA甲基化水平与TCF21基因mRNA表达水平呈显著正相关（R2区域：r=0.438，P<0.05；R3区域：r=0.371，P<0.05；R2+R3区域：r=0.489，P<0.05）；R2区域的DNA甲基化显著抑制其转录活性（P<0.05）。综上所述，TCF21基因在高、低脂系肉鸡腹部脂肪组织中的表达水平主要与其启动子R2区域的DNA甲基化水平有关。     

小型猪乳化异氟醚全身麻醉效果监测

为探讨乳化异氟醚进行小型猪全身麻醉麻醉效果,选取14头巴马猪以2.80 mL.kg-1.h-1进行静脉麻醉,在麻醉过程中对生理反射、镇痛、镇静、肌松,呼吸和循环系统进行监测。结果表明,乳化异氟醚可以使小型猪维持良好外科麻醉状态,麻醉过程中小型猪一般生理指标、循环系统指标未发生显著变化(P>0.05),呼吸系统指标与正常比较有显著性改变(P<0.05或P<0.01),但均在安全范围内,小型猪麻醉过程无异常表现。乳化异氟醚能满足临床手术麻醉需要,可以作为小型猪全身麻醉药物推广应用。     

丁酸梭菌对哺乳母猪繁殖性能、免疫和抗氧化功能的影响

本试验旨在研究丁酸梭菌对哺乳母猪免疫和抗氧化的影响,选取健康经产长白母猪32头(胎次和预产期相近),随机分为4组,每组8个重复,每个重复1头。对照组饲喂基础日粮,试验组分别在基础日粮基础上添加250、500、1000 mg/kg丁酸梭菌。预试期7 d,试验期为28 d(产前7 d至21 d断奶)。结果表明:哺乳第1天,1000 mg/kg丁酸梭菌组血清中IgA、IgM和IgG含量均显著高于对照组;哺乳第21天,250、500、1000 mg/kg丁酸梭菌组IgA含量均显著高于对照组,500 mg/kg丁酸梭菌组IgM和IgG含量均为最高;哺乳第1、10、21天,1000 mg/kg丁酸梭菌组IL-6和IL-1β含量较对照组均显著降低;哺乳第1、10、21天,各丁酸梭菌组超氧化物歧化酶活性均显著高于对照组,丙二醛含量均显著低于对照组;哺乳第1、21天,500、1000 mg/kg丁酸梭菌组的过氧化氢酶含量较对照组均显著升高;哺乳第21天,500 mg/kg丁酸梭菌组总抗氧化能力和谷胱甘肽过氧化物酶活性显著升高;哺乳第1天和第21天,1000 mg/kg丁酸梭菌组粪便中大肠杆菌的数量显著低于对照组。由此得出,在妊娠后期和哺乳期饲粮中添加丁酸梭菌可以显著提高母猪免疫功能和抗氧化能力,降低粪便中大肠杆菌的数量,且添加量为500~1000 mg/kg时效果明显。