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Shoma MIKAWA Yasuhiro OHTA Noriyuki KAJI Md Shafiqul ISLAM Takahisa MURATA Hiroshi OZAKI Masatoshi HORI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(11):1443-1449
Endotoxin causes gastrointestinal motility disorder. Aim of this study is to clarify
inhibitory mechanisms of lipopolysaccharide (LPS) on smooth muscle contraction in rat
ileum. Ileal tissues were isolated from control rat or from LPS-induced peritonitis model
rat. Treatment with LPS inhibited carbachol (CCh)-mediated contraction in a time-dependent
manner. Cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) genes were
also upregulated, but iNOS expression was preceded by a rising of COX-2. All subtypes of
prostaglandin E2 (PGE2) receptors (EP1-EP4) were expressed in ileum,
and PGE2 and selective EP2 or EP4 agonist inhibited CCh-mediated contraction.
Selective iNOS inhibitor did not reverse LPS-induced inhibition of contraction by CCh at 1
and 2 hr, but reduced the inhibitory action at 4 hr after the LPS treatment. COX-2
inhibitor reversed the inhibitory action by LPS in all exposure time. Finally, in ileal
tissues isolated from peritonitis model rat, iNOS expression was upregulated only at 4 hr
after LPS administration, resulting in enhanced inhibitory action of LPS against
CCh-induced contraction. In conclusion, LPS induces COX-2 to produce PGE2,
which initially activates EP2 and/or EP4 on smooth muscle cells to inhibit the
contractility in early phase of LPS exposure. Moreover, in late phase of LPS treatment,
iNOS is expressed to produce NO, which in turn inhibited the contraction by CCh. The
inhibitory cascade is similar in the ileum isolated from peritonitis model rat, indicating
time-dependent changes of inhibitory action by LPS on intestinal motility in
peritonitis. 相似文献
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Minoru OHTA Manabu NEMOTO Koji TSUJIMURA Takashi KONDO Tomio MATSUMURA 《Journal of Equine Science》2011,22(3):53-56
A PCR assay for the diagnosis of respiratory disease induced by equine herpesvirus type 1
(EHV-1) was performed at the clinical laboratory in the Racehorse Clinic of the Ritto
Training Center of the Japan Racing Association from December 2007 to March 2008. The
assay was performed without the trouble of contamination throughout the study and its
turnaround time was approximately 6 hr. The PCR detection rates of EHV-1 among
seroconverted horses were 22.2% for nasal swabs and 33.3% for blood samples. However,
EHV-1 DNA was also detected in horses without seroconversion at a low rate. These results
indicated that the PCR assay should be used as an adjunct method, but would help to make
an early diagnosis of EHV-1 infection. 相似文献
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Tatsuyuki YOSHIDA Taro SATO Koji CHIBA Itaru YOSHIMURA Masahiro OSADA Konosuke OKADA Hiroki FURUTA Akihiko OHTA Hitoshi USHIJIMA 《Animal Science Journal》2012,83(7):529-534
This study was designed to evaluate the effect of estrus synchronization treatments on recipient heifers for embryo transfer (ET). Holstein heifers were separated into the following three groups: (i) an administration of 50 µg GnRH (gonadotropin‐releasing hormone) analog was given to heifers at a random stage of the estrus cycle, followed 7 days later by two administrations of 7.5 mg prostaglandin F2 alfa analog (PG) as control; (ii) another administration of 100 µg GnRH was given to the control group at 48 h after the administration of PG as the second GnRH group; and (iii) an administration of 0.75 mg estradiol benzoate (E2) was given to the control group at 24 h after the administration of PG as the E2 group. Each method caused estrus synchronization. Fresh embryos were nonsurgically transferred into the suitable recipients that had a functional corpora lutea (CL) 7 days after estrus. The E2 group showed a significantly higher (P < 0.01) rate of estrus synchronization (98.9%) at 1–3 days after PG administration and the final pregnancy rate of the E2 group (50.6%) was also significantly higher than the other groups (37.1%, P < 0.05 and 30.9%, P < 0.01, respectively). These findings demonstrate that E2 administration 24 h after PG protocol is effective for estrus synchronization of Holstein heifers, thus improving the productivity of ET. 相似文献
24.
Rommaneeya LEELA-ARPORN Hiroshi OHTA Genya SHIMBO Noboru SASAKI Mitsuyoshi TAKIGUCHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(9):1459
The size of canine focal liver lesions (FLLs) is known to be one of the predicting criteria for malignancy. However, there are discrepancies for the measurement of maximum lesion size, resulting in contradicting results among studies and incidences of false positive outcomes. Thus far, the morphometric changes of FLLs for distinguishing malignancy from benignancy remains undocumented. This study aimed to investigate morphometric characteristics of FLLs using computed tomography (CT). CT images of 40 dogs with histopathological confirmation of 49 liver lesions, including 39 hepatocellular carcinomas and 10 nodular hyperplasias were retrospectively reviewed. The morphometric parameters including size (long and short axis diameters measured on transverse image), shape (measured by long to short axis (L/S) ratio), volume, and surface appearance of a liver lesion were evaluated using univariate and stepwise multivariate analyses, respectively. The results of univariate analysis showed that long and short axis diameters, L/S ratio, volume, and surface appearance of a lesion were significantly different between hepatocellular carcinomas and nodular hyperplasias. Multivariate analysis revealed that short axis diameter (>3.30 cm; odds ratio (OR): 36.1, 95% confidence interval (CI): 3.36–387.05, P=0.0031) and L/S ratio (>1.23; OR: 18.1, 95% CI: 1.61–205.12, P=0.0191) were independent predictors of malignancy, with the area under the curve of 0.9154. These results suggest that the combination of short axis diameter and L/S ratio is a promising tool for predicting liver malignancy with outstanding discriminating ability. 相似文献
25.
Changes in fertility of rainbow trout eggs retained in coelom 总被引:1,自引:0,他引:1
TERUO AZUMA HIROMI OHTA SABURO ODA KOJI MUTO TAKASHI YADA TATSUYA UNUMA 《Fisheries Science》2003,69(1):131-136
ABSTRACT: Effects of prolonged retention time of ovulated eggs in the parental coelom on fertilization success were studied in rainbow trout Oncorhynchus mykiss using cryopreserved sperm with a uniform fertilizing ability. Proportions of successful fertilization, eyed eggs and hatched alevins were examined at different time periods up to a retention time of 14 days beyond the ordinary stripping time, and were compared with eggs incubated in artificial coelomic fluids (ACF). Eggs that were retained longer in the coelom showed gradual decreases in all the proportions of successful fertilization, eyed eggs and hatched alevins. The progress of cleavage after fertilization slowed with prolonged retention times. Eggs incubated in ACF lost their fertilizing ability much sooner than those retained in the coelom. The hatching rate of eggs retained for 2 weeks in coelom was 36%, while it was 1% in those eggs incubated for 4 days in ACF. Thus, eggs retained in the coelom showed higher fertilization success than those incubated in ACF. 相似文献
26.
Hiroshi OHTA Kanae TAKADA Yuji SUNDEN Yu TAMURA Tatsuyuki OSUGA Sue Yee LIM Masahiro MURAKAMI Noboru SASAKI Bandula Kumara WICKRAMASEKARA RAJAPAKSHAGE Kensuke NAKAMURA Masahiro YAMASAKI Mitsuyoshi TAKIGUCHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(3):409-414
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Koji NAKADE Ryosuke KAMISHIMA Yusuke INOUE Abdulatef AHHMED Satoshi KAWAHARA Tatsuo NAKAYAMA Masugi MARUYAMA Masahiro NUMATA Kazuyoshi OHTA Takayoshi AOKI Michio MUGURUMA 《Animal Science Journal》2008,79(6):710-715
A novel angiotensin‐converting enzyme (ACE) inhibitory peptide was isolated and purified from chicken bone extract by enzymatic digestion. The peptide was defined as an ACE inhibitor, and it demonstrated antihypertensive activity following oral administration to spontaneously hypertensive rats (SHRs). The results of this study suggest that peptides derived from an extract of chicken bones, administered orally, have the ability to reduce the blood pressure of SHRs significantly over a short period of time (3 h). Moreover, the blood pressure then remains low for 3 h. This peptide derived from chicken bones may therefore have great value as a short‐term remedy for chronic conditions such as high blood pressure. The amino acid sequence of the peptide was YYRA (Tyr‐Tyr‐Arg‐Ala), which was the origin of the Ig heavy chain V region (27–30 position). The IC50 value of its synthetic peptide was 33.9 μg/mL. We suggest that the ACE inhibitory and antihypertensive peptides derived from chicken bone extract may contribute to develop physiologically functional foods or improve food functionality. 相似文献