Identification of a Group of Novel y-Gliadin Genes

γ-Gliadins are an important component of wheat seed storage proteins. Four novel γ-gliadin genes （Gli-ngl to Gli-ng4） were cloned from wheat （Triticum aestivum） and Aegilops species. The novel γ-gliadins were much smaller in molecular size when compared to the typical γ-gliadins, which was caused by deletion of the non-repetitive domain, glutamine-rich region, 3＂ part of the repetitive domain, and 5＇ part of the C-terminal, possibly due to illegitimate recombination between the repetitive domain and the C-terminal. As a result, Gli-ngl and Gli-ng4 only contained two and three cysteine residues, respectively. Gli-ngl, as the representative of novel γ-gliadin genes, has been sub-cloned into an Escherichia coli expression system. SDS- PAGE indicated that the both cysteine residues of Gli-ngl could participate in the formation of intermolecular disulphide bonds in vitro. Successful cloning of Gli-ngl from seed cDNA of T. aestivum cv. Chinese Spring suggested that these novel γ-gliadin genes were normally transcribed during the development of seeds. Phylogenic analysis indicated that the four novel γ-gliadin genes had a closer relationship with those from the B （S） genome of wheat.     

~(60)Coγ射线对甘薯黑斑病(Ceratocystis fimbriata)的抑制效果

用60~(Co)γ射线25—400krad的不同剂量辐照甘著黑斑病菌及带病甘薯(Ipomoeabatatas Lam.)薯块,发现200及400krad剂量的辐照对菌丝体和子囊孢子有一定的抑制作用,对分生孢子、厚垣孢子抑制作用甚微;200krad在20天内基本抑制薯块上病斑的发生和发展,薯块外观良好,接种发病率比对照低75％,病斑平均直径不及对照的五分之一;400krad的抑茵效果更高,但尚不能完全杀死病菌孢子,并对薯块外表有伤害,使其色泽变暗,贮藏后期易产生干腐。本试验表明,200krad剂量的辐照处理对甘著贮藏有实践意义。     

木材光引发自由基规律的研究

木材在~(60)Co γ射线辐射下引发g值为2.019、2.029、2.027、2.016、1.999等几种自由基。ESR主谱自由基浓度远大于机械自由基。并与树种、辐射剂量等有关。主谱自由基相对强度随剂量x按指数率Y=1-e~(-ax)上升,当剂量增加到100KGy时趋于饱和,根据福建21个常见树种测试有明显的规律性。     

γ射线辐射对木材构造和材性的影响

该文在归纳高能射线的种类和高能射线辐射对木材作用机理的基础上 ,详细地归纳了高能射线 ,特别是γ射线辐射对木材构造和木材化学、物理、力学性质的影响 .本质上 ,当射线辐射剂量较高时 ,将引起木材细胞壁结晶度降低 ,细胞壁主成分分子链切断 ;当射线辐射剂量较低时 ,可能导致细胞壁主成分产生交联 .在此基础上 ,作者提出了利用γ射线辐射固定木材变定和研究木材变定永久固定机理的构想     

富γ-氨基丁酸茶的药理功能研究进展

综述了富γ-氨基丁酸茶的药理功能和γ-氨基丁酸富集方法的研究进展,提出应进一步改进和完善富γ-氨基丁酸茶的加工工艺和我国富γ-氨基丁酸茶叶产品的广阔市场前景。     

IFN-γ对妊娠早期大鼠下丘脑中IL-18表达的影响

为探讨y干扰素(IFN-y)对妊娠早期大鼠下丘脑中白细胞介素-18(IL-18)表达及其对妊娠结局的影响,将妊娠9d的SD大鼠随机分为对照组、试验1组和试验2组,采用生殖道肌肉注射的方法,对照组大鼠生殖道肌肉注射生理盐水,试验组大鼠注射IFN-y,其中1 组剂量为2.5×104 U/只,2组剂量为7.5×104 U/只...     

马铃薯中γ-氨基丁酸富集技术研究

以马铃薯(Solanum tuberosum)为对象,研究厌氧环境、好氧环境、厌氧-有氧交替处理、发芽诱导、低温冷冻诱导等方法富集γ-氨基丁酸(GABA)。测定富集GABA在35℃样品的γ-氨基丁酸和谷氨酸含量,评价各种富集方法的效果。马铃薯厌氧-有氧交替富集的最优工艺为在35℃厌氧处理8 h,再好氧处理3 h,最后厌氧处理13 h,样品GABA含量增长到82.3±2.5 mg/100 g,是对照的将近2.6倍。发芽富集GABA含量是对照的1.3倍;冷冻富集GABA后微波加热解冻含量是对照的1.74倍。结果表明,马铃薯使用厌氧-好氧法富集GABA是合适的;发芽富集法不适合;低温冷冻法采用速冻才适宜,且要配合适宜解冻方案。     

富硒和γ-氨基丁酸萌芽糙薏米培养液组分的工艺优化

为提高糙薏米中γ-氨基丁酸的富集量,采用响应面试验法对糙薏米的培养液组分进行工艺优化。结果表明:培养液中Na2SeO3浓度以10mg/L为宜,其发芽率最高,达(90.33±1.73)%;在Na2SeO3浓度为10mg/L的培养液中辅以11mmol/L CaCl2、87.7μmol/L GA3和31.5mmol/L MSG进行萌芽处理时,萌芽糙薏米中总硒、有机硒和GABA含量分别达0.42mg/100g、0.37mg/100g和116.69mg/100g,是一种具高值化产品开发潜力的保健食品基料。     

~（60）Co γ射线不同剂量辐射甘蔗种芽试验初报

采用２０００γ、３５００γ、５０００γ、６５００γ、８０００γ５个等级的６０Ｃｏγ射线对甘蔗种芽进行电离辐射处理，观察对甘蔗生长发育的影响及苗期辐射形态变化，发现随着辐射剂量的增加出苗率明显下降，生长亦严重受阻，并发生矮化苗，茎色改变，心叶不抽出，株型变化等多种辐射形态变化。３５００γ以上的处理总变化数均在５０％以上，尤以５０００γ以上处理总变化率高，但差异不显著。初步认为蔗芽辐射育种的剂量范围为３５００－８０００γ，尤以３５００－６５００γ１为宜。     

Propagation in vitro of pear, Pyrus communis L., cultivars ‘William's Bon Chrétien’, ‘Packham's Triumph’ and ‘Beurré Bosc’

Shoot cultures were established from nodal explants of 3 pear cultivars by subculture on Murashige and Skoog medium containing mixtures of 3 cytokinins. Zeatin and 6·(γ,γ-dimethylallylamino)purine were both supplied at 10 μM and benzyladenine as follows: ‘William's Bon Chrétien’, 6 μM; ‘Packham's Triumph’, 8 μM; ‘Beurré Bosc’, 10 μM. Production of axillary shoots was greater by basal explants (4–6) comprising the remnants of the previous subculture than by apical explants (2–3) comprising the distal parts of extension shoots. Up to 80% of microcuttings of all cultivars formed roots in vitro with either γ-indolebutyric acid, 10 μM, or α-naphthaleneacetic acid, 10 μM, and approximately 50% of rooted microcuttings were successfully established as container-grown plants. Use of aseptic methods for producing own-rooted pears is discussed in relation to fruit growing and fruit improvement.