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101.
采用放射免疫分析测定法(RIA)对人工繁殖的中华鲟(Acipenser sinensis)早期发育阶段中17β-雌二醇(17β-estradiol,E2)和睾酮(testosterone,T)的整体水平进行了测定。实验共测定了4批次(分别来自4尾雌鲟)受精卵及仔鱼的E2和T水平,结果表明:中华鲟胚胎发育期间E2水平在小范围内波动,T水平除胚胎发育早期出现上升外总体呈下降趋势;仔鱼出膜后E2水平开始逐渐上升,T水平是先降低后又逐渐升高。其中第1批次的测定结果(单位为pg/gtissue)为:胚胎发育期间E2水平在1.37~7.38之间波动,仔鱼出膜后3d为5.21,出膜后9d为37.26;胚胎发育期间T水平最低为293.58,最高为1420.41,仔鱼出膜后4d为117.96,出膜后7d为27.25,出膜后9d为148.48。对于仔鱼出膜后体内高水平的E2和T的来源,推测其可能与仔鱼自身合成性类固醇激素有关。另外,本实验中还发现,质量较差的卵子T水平明显低于质量较好者。 相似文献
102.
Initial appearance and development of Leydig cells (LCs) during testicular differentiation in tilapia,Oreochromis niloticus, were investigated histologically. In addition, changes of testosterone levels in gonadal tissue and serum were examined
by radioimmunoassay. In the gonads of fry at 23–26 days after hatching, initial testicular differentiation was confirmed by
the observation of the differentiation of connective tissues into tissues which are characteristic of the adult testis. LCs,
which were identified by the ultrastructural features (a moderate number of mitochondria with tubular cristae, well developed
smooth endoplasmic reticulum and many free ribosomes) appeared initially at the time of testicular differentiation. LCs increased
in number rapidly in the testes of fish at 70 days after hatching. Concomitant with this increase, spermatogonia increased
in number. Testosterone was detectable in the fish at 40–50 days after hatching, but levels in tissue and serum were low.
Testosterone levels increased gradually in the fish beginning at 70 days after hatching and increased still more at 100–150
days accompanying active spermatogenesis. 相似文献
103.
The upstream migration of adult anadromous fishes is characterized by physiological changes in responses to reproductive and
energetic challenges. This study analyzed the physiological responses of lake-resident anadromous masu salmon (Oncorhynchus masou) and sockeye salmon (O. nerka) to migration in order to determine if these fish might serve as a suitable model for ocean-running populations and to differentiate
between physiological responses to reproduction and to exercise-linked aspects of migration. Reproductive (estradiol, testosterone,
11-ketotestosterone, 17α,20β-dihydroxy-4-pregnen-3-one) and metabolically-linked (thyroxine, triiodothyronine) hormones showed
similar patterns to ocean-running anadromous populations. White muscle pyruvate kinase, lactate dehydrogenase and malate dehydrogenase
decreased with the onset of spawning season while white muscle citrate synthase, β-hydroxyacetyl CoA dehydrogenase, phosphofructokinase
and glutamate oxaloacetate transaminase did not, suggesting that the former group of enzymes are responding to reproductive
or food intake signals while the second group, which typically change during anadromous migration, may be responding to exercise-linked
aspects of migration.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
104.
105.
The glutamate agonist, N-methyl-D,L-aspartate (NMA) stimulates the secretion of growth hormone (GH) from pituitary fragments
in vitro and increases plasma GH levels in vivo in rainbow trout, Oncorhynchus mykiss (Flett et al. 1994; Holloway and Leatherland
1997a,b); however gonadal steroid hormones appear to modulate this response in experimental situations. This study examines
whether steroid hormones also modulate the GH-regulatory actions of NMA during the normal reproductive cycle of rainbow trout
by examining the relationship between the stage of sexual maturation and the pituitary release of GH in vitro in response
to an NMA (10-8 M) challenge. NMA had no effect on mean GH release from the pituitary glands of fish that were immature (GSI <1.0), from
males during early development (GSI 1.0-3.0), or from sexually mature males (with free running milt) and females (ovulated).
However, NMA significantly increased GH release from pituitary glands taken from females during the early stages of gonadal
growth (GSI 1.0-9.0) and from males and females sampled during the later stages of gonadal growth (males GSI 3.01-6.0; females
GSI 9.01-15.0). The GH-stimulatory action of NMA in males and females progressed to a maximum effect during the late stages
of gonadal growth, and disappeared in ovulated females and free running males. Moreover, in female fish, the maximal GH release
in response to the NMA challenge is positively correlated with plasma 17β-estradiol levels; no such correlation was evident
for plasma testosterone levels in males. Changes in the GH response to NMA during maturation while gonadal steroid levels
fluctuate provides further evidence to suggest that the effects of NMA on GH secretion are intimately linked to endogenous
gonadal steroid hormone levels.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
106.
西伯利亚鲟不同性别与卵巢发育时期血液性类固醇激素的差异与判别分析 总被引:3,自引:2,他引:3
利用电化学发光免疫分析法(ECLIA)测定了人工养殖西伯利亚鲟(Acipenser baerii baerii)不同性别和卵巢发育时期血浆中睾酮(T)和雌二醇(E2)的含量。利用活组织取样的方法,根据性别和卵子颜色及大小将实验鱼分为成熟雄鱼(M)、雌鱼Ⅲ期、Ⅳ期和Ⅴ期共4个组。研究结果表明,西伯利亚鲟血浆T含量雄鱼高于雌鱼(P<0.01),且雄鱼与雌鱼Ⅲ期和Ⅴ期组有极显著性差异(P<0.01),而E2含量除与雌鱼Ⅳ期有极显著性差异外(P<0.01),与雌鱼Ⅲ期和Ⅴ期组无显著性差异(P>0.05),雄鱼表现为T含量和T/E2比值高,而E2含量低。雌鱼随卵巢的发育T和E2含量均表现为"先上升,后下降"的趋势,即Ⅳ期组性类固醇激素的含量明显高于Ⅲ期和Ⅴ期,且差异极显著(P<0.01)。根据血浆中T和E2的含量建立了西伯利亚鲟性别和性腺发育时期的4个判别函数:YM=-11.322+0.043T+0.153E2,YⅢ=-1.390+0.000T+0.061E2,YⅣ=-18.074+0.032T+3.305E2,YⅤ=-2.316+0.785T+0.008E2,经检验总体判别准确率为95%,其中对性别的判别准确率为100%,对雌鱼卵巢发育时期的判别准确率为93%。 相似文献
107.
Shinya Sato Shugo Suzuki Aya Naiki-Ito Masami Komiya Long Ne Hiroyuki Kato Hiroyuki Sagawa Yoriko Yamashita Tomoyuki Shirai Satoru Takahashi 《Journal of toxicologic pathology》2014,27(1):43-49
We have established a transgenic rat for adenocarcinoma of the prostate (TRAP) model that features uniform adenocarcinoma development in prostatic lobes at high incidence within a short experimental period. However, no invasive carcinomas with reactive stroma characteristics similar to those in man were observed. We therefore have focused on a new model for invasive carcinoma of the prostate using TRAP rats. In experiment 1, male TRAP rats in groups 1 and 2 were treated with orchiectomy at day 0 of the experiment. Rats in groups 1–3 underwent testosterone propionate (TP) implantation from weeks 1 to 4 and from weeks 6 to 16. Rats in groups 1 and 3 were given 3,2’-dimethyl-4-aminobiphenyl (DMAB) after TP implantation. The rats of group 4 served as controls. In experiment 2, the rats were divided into three groups, none of which received DMAB or orchiectomy, treated with TP continuously or with the treatment withdrawn once or twice. In experiment 1, invasive adenocarcinomas with abundant collagenous stroma were found in the dorsolateral and anterior prostate, some of which showed perineural space invasion at week 16. The number of invasive carcinoma foci was most frequent in group 3. In experiment 2, invasive adenocarcinoma development in the lateral prostates was correlated with the number of TP administration/withdrawal cycles. In conclusion, our newly established rat model for invasive adenocarcinoma of the prostate could serve as a useful preclinical model for evaluating the in vivo efficacy of preventive and therapeutic agents targeting of the tumor microenvironment. 相似文献
108.
采用活体注射和卵巢体外培养添加多氯联苯(PCB1254)两种方式研究了PCB1254对异育银鲫血清中和离体卵巢分泌睾酮及雌二醇的影响。注射PCB1254后,血清中睾酮浓度随作用时间延长而降低,随PCB1254浓度的增加而减小;血清中雌二醇随作用时间延长而增加,随PCB1254浓度增加而增加。体外培养时卵巢时,培养液中雌二醇含量随培养时间延长而增加,随PCB1254浓度增加而增加。结果表明PCB1254促进雌二醇分泌,抑
制睾酮分泌。 相似文献
109.
No significant differences in plasma testosterone level were observed between cows carrying a male foetus and cows carrying a female foetus at any ten-day interval from day 35 of gestation until parturition. Reported higher abortion rates for male than for female foetuses would thus appear not to be due to effects of foetal testosterone on the maternal endocrine balance. In spite of a great individual variation in plasma testosterone values at similar stages of gestation, certain trends are evident. From the 35th to the 80th day of gestation the average concentration was 90–100 pg/ml. Later it rose and reached 200 pg/ml on the 180th day, remaining at this level until after partus. During the first day after parturition plasma testosterone fell significantly and stabilized around 120 pg/ml. 相似文献
110.
Levels of peripheral plasma testosterone and LH were studied in 4 bulls hourly during a 12 hr. sampling period at 5 times of the year. The average plasma testosterone levels were significantly lower in October (1.8 ng/ml, Ρ < 0.001) and December (2.5 ng/ml, Ρ < 0.05) than in February, June and August (3.5, 3.7 and 3.7 ng/ml respectively). LH showed a slight fluctuation during the day, with values ranging between 0.8 and 3.8 ng/ml, but underwent no significant seasonal variation. A significant increase in average plasma testosterone was observed 1 hr. after the LH peaks (P < 0.001). 相似文献