HPLC-DAD法测定复方麻黄散中非法添加喹烯酮

建立了复方麻黄散中非法添加喹烯酮的HPLC-DAD检查方法。以C₁₈柱为固定相,甲醇-水(60∶40,V∶V)为流动相,流速为1.0 mL/min,柱温30℃,二极管阵列检测器检测,采集波长范围为190～400 nm,提取波长为314 nm。通过液相色谱保留时间、紫外吸收光谱和峰纯度分析对非法添加物进行确认。结果表明,该色谱条件下喹烯酮与其他物质分离良好。喹烯酮在2.0～100.0μg/mL范围内线性关系良好,R²=0.9999。喹烯酮在复方麻黄散中的平均回收率为101.4%,RSD为0.5%;方法的检测限为0.25 g/kg。该方法简便,准确,可靠,可以用于检查复方麻黄散中非法添加喹烯酮。     

HPLC法测定复方甲氧沙林洗剂中甲氧沙林和醋酸地塞米松的含量

本文采用高校液相测定了复方甲氧沙林洗剂中醋酸地塞米松和甲氧沙林的含量,固定相为:安捷伦Cl8(4.6mm×250mm,5um),甲醇-乙腈-水(40:30:30)为流动相,检测波长为240nm;甲氧沙林在10~80mg·m L-1范围内呈良好的线性关系;醋酸地塞米松在20~100mg·m L-1范围内呈良好的线性关系。甲氧沙林和醋酸地塞米松的平均回收率分别为99.6%、99.6%,RSD为0.81%(n=6)、0.83%(n=6);此方法简便、准确、重现性好,可用于复方甲氧沙林洗剂中甲氧沙林和醋酸地塞米松的含量测定。     

Effects of Compound Chinese Medicine on Growth Performance,Nutrients Apparent Digestibility,Immune Function and Slaughter Performance of Weaning Rex Rabbits

This experiment was conducted to study the effects of different levels of compound Chinese medicine on growth performance,nutrients apparent digestibility,immune function and slaughter performance of weaning Rex rabbits.70 healthy Rex rabbits at age of 60 days were divided into 7 groups with 5 replicates per group and 2 rabbits per replicate.Group Ⅰ was fed with basic diet as control group,group Ⅱ was fed the basal diet supplemented with antibiotic (contains 50 mg/kg milligrams bacitracin zinc,10 mg/kg colistin sulfate).The other five groups (Ⅲ,Ⅳ,Ⅴ,Ⅵ and Ⅶ) were fed the basal diet supplemented with 1%,2%,3%,4%,5% compound Chinese medicine,respectively.The pre-test period lasted for 7 days and the trial period lasted for 45 days.The results showed as follows:①The ADG in group Ⅳ was significantly higher than that in groups Ⅰ,Ⅱ and Ⅲ (P< 0.05),the F/G in group Ⅳ was significantly lower than that in other groups (P< 0.05).②The apparent digestibility of DM in group Ⅳ was significantly higher than that in groups Ⅰand Ⅱ (P< 0.05),the apparent digestibility of DM in group Ⅶ was significantly higher than that in group Ⅰ (P< 0.05).The apparent digestibility of CF in group Ⅳ was extremely significantly higher than that in group Ⅰ (P< 0.01),the apparent digestibility of CF in group Ⅶ was significantly higher than that in group Ⅰ (P< 0.05).The apparent digestibility of NDF in group Ⅳ was extremely significantly higher than that in group Ⅰ (P< 0.01),and significantly higher than that in groups Ⅱ,Ⅲ,Ⅴ and Ⅶ (P< 0.05).The apparent digestibility of ADF in group Ⅳ was significantly higher than that in groups Ⅰ,Ⅱ,Ⅴ and Ⅶ (P< 0.05).The apparent digestibility of CP in group Ⅳ was significantly higher than that in groups Ⅰ and Ⅱ (P< 0.05).③ There were no significant differences in the contents of IgG,IgA and IgM of serum among all groups (P> 0.05),the spleen index in group Ⅳ was significantly higher than that in groups Ⅰ,Ⅱ,Ⅲ and Ⅴ (P< 0.05).④There were no significant differences in the slaughter rate,half eviscerated slaughter rate and all eviscerated slaughter rate among all groups (P> 0.05).In summary,adding compound Chinese medicine and antibiotics in the feed both could improve the growth performance,nutrients apparent digestibility,immune function and slaughter performance of weaning Rex rabbits,and the appropriate compound Chinese medicine level in the diet of weaning Rex rabbits was 2%.     

复方沙棘养血膏抗辐射作用的实验研究初报

用一定剂量的~(60)Co—γ射线照射小鼠造成放射性损伤,用复方沙棘养血膏进行治疗.以复方阿胶浆为阳性对照药。实验结果表明:复方沙棘养血膏能明显提高受辐射小鼠外周血白细胞和红细胞数量;提高骨髓有核细胞数;对脾脏系数也有一定的影响。     

中药增效抗菌复方对雏鸡大肠杆菌病的临床研究

为了验证中药增效抗菌复方在临床上对大肠杆菌病的作用效果,试验选取5个发生雏鸡大肠杆菌病的鸡场研究其临床治疗效果。结果表明：每只鸡每天饮水给予中药增效抗菌复方2 g,连用7 d,平均治愈率为84.0%。说明中药增效抗菌复方对鸡大肠杆菌病具有确切的疗效,为中药增效抗菌复方的临床推广应用奠定了基础。     

中药复方舒肤膏质量标准研究

采用薄层色谱法对荆芥、防风、凌霄花和狼毒进行定性鉴别,采用高效液相色谱法对中药复方舒肤膏的有效成分梣酮和黄柏酮进行含量测定。结果表明,薄层色谱法结果理想,阴性样品无干扰;在5.92～47.36μg/mL梣酮与峰面积线性关系良好(r=0.999 1),平均回收率为99.76%(RSD=1.34%),在10.2～81.6μg/mL黄柏酮与峰面积线性关系良好(r=0.999 0),平均回收率为99.89%(RSD=1.63%),表明该方法操作简便,专属性好,准确可靠,可用于中药复方舒肤膏的质量控制。     

银翘复方药渣发酵处理试栽毛头鬼伞及主要药物成分分析

为回收利用生产银翘片废弃药渣,以毛头鬼伞为供试菌株,发酵处理银翘药渣;建堆配料设计原则为控制总料C/N接近40∶1,以玉米芯为主料(配合添加奶牛粪、尿素),逐渐增大银翘药渣用量,以替代玉米芯;堆料共采用4个配方,配方A(CK):玉米芯200kg,牛粪100kg,尿素3.5kg,辅料6%(生石灰2%、石膏2%、过磷酸钙2%);配方B:玉米芯100kg,银翘药渣50kg,牛粪50kg,尿素1.0kg,辅料6%;配方C:玉米芯75kg,银翘药渣100kg,牛粪50kg,辅料6%;配方D:玉米芯50kg,银翘药渣150kg,牛粪50kg,辅料6%。4个配方堆料总干质量均在200kg以上,依墙建堆成半圆锥形(h≥1.0m,r≥1.4m);发酵升温14d(4-3-3-2-2)、经4次翻堆后,摊料降温装袋接种,待菌丝满袋后脱袋覆土出菇。结果表明:以配方C的产出率为最高,其相应生产指标为,初始C/N为41.66、终点C/N为23.71、腐熟度T为0.569、接种满袋率93.7%、菌袋平均产量381.9g·kg~(-1)、生物学效率41.0%。经HPLC检测,配方C发酵料中绿原酸、牛蒡苷含量分别为5.20~5.40、4.49~5.30μg·g~(-1),其栽培所得子实体中绿原酸、牛蒡苷含量分别为0.40~0.45、3.81~4.27μg·g~(-1),其中牛蒡苷从栽培料到子实体的迁移率达84.93%。     

紫锥菊复方对断奶仔猪生产性能和免疫功能的影响

在饲料中添加紫锥菊复方观察其对断奶仔猪的生长性能和免疫功能的影响。结果表明,添加高剂量(质量分数为1.5%)紫锥菊复方组仔猪的日增重、血清中淋巴细胞阳性率、猪瘟抗体效价、猪繁殖与呼吸综合征抗体效价等均比其他组有显著提高(P0.05),腹泻率均比其他组显著降低(P0.05),表明紫锥菊复方在一定程度上可以改善仔猪的生产性能,提高机体的免疫力。     

一个黄酮成分复方对鸭病毒性肝炎的治疗作用

为了探究一个黄酮成分复方对鸭病毒性肝炎(duck virus hepatitis,DVH)的治疗作用。采用肌肉注射鸭肝炎病毒(duck hepatitis A virus,DHAV)制造DVH模型,雏鸭饮服黄酮成分复方予以治疗,通过对比空白对照组、病毒对照组和治疗组雏鸭存活率、肝损伤指标(肝脏眼观病理变化和肝损伤生化评价指标)、炎症因子(IL-6和IL-8)和肝细胞生长因子(hepatocyte growth factor,HGF)的动态变化,来研究该复方对DVH的治疗作用。结果显示,黄酮复方可以显著减轻染病雏鸭的肝损伤,显著提高炎症因子和HGF的分泌水平,显著地提高了染病雏鸭的存活率。     

高效液相色谱法测定复方氨基酸注射液中的酪氨酸和色氨酸含量

本文采用高校液相测定了复方氨基酸注射液中酪氨酸和色氨酸的含量，固定相为：安捷伦Cl8(4.6mm×250mm，5um)，甲醇-乙腈-0.1%醋酸钠水溶液(3：5：92)为流动相，检测波长为280nm；色氨酸在50~150μg·mL-1范围内呈良好的线性关系；酪氨酸在100~900μg·mL-1范围内呈良好的线性关系。色氨酸和酪氨酸的平均回收率分别为99.5%、99.5%，RSD为0.63%(n=6)、0.56%(n=6)；此方法简便、准确、重现性好，可用于复方氨基酸注射液中色氨酸和酪氨酸的含量测定。