Ileal indigestible protein may be an important factor for the occurrence of post weaning diarrhoea in piglets. Depending on the quantity of indigested protein, an imbalance in the microbial community can occur, resulting in an overgrowth of pathogens. Diet viscosity may influence nutrient digestibility and substrate availability for microbial growth. Therefore, we examined the effect of dietary viscosity in piglet nutrition. Carboxymethylcellulose (CMC) was added as a model substance in order to increase diet viscosity. The effect of 1% supplemented CMC on nutrient digestibility, morphological and physiological parameters was measured in piglets 3 weeks post weaning at 26 days of age. The main effects of inclusion of CMC were:
– A 10% slower gastric emptying rate (P = 0.18).
– An increased protein hydrolysis in the gastric fraction, measured as increased nitrogen concentration in the liquid phase of gastric contents (26% in control vs. 34% in experimental group; P = 0.13).
– An increased production of the protein hydrolysing enzyme amino-peptidase from 359 U/g protein for the control diet to 516 U/g protein for the experimental diet (P < 0.01).
– A numerical increase in apparent ileal protein digestibility by 6% from 75.2% to 79.5% (P = 0.22) and a significant increase in faecal protein digestibility by 5% (P = 0.044).
These results indicate that an optimized diet viscosity might be one factor exerting a beneficial influence on protein digestibility. We hypothesize that the effect of diet viscosity on gastric emptying rate is the major factor. 相似文献
Gut environment and microflora, digestibility, and performance were studied in weaned piglets in Central Vietnam fed a dry control diet (CO), a naturally fermented liquid diet (FE), and a liquid diet with inclusion of rice distiller's residue (RDR). Digesta samples were collected at the start (day 0) and at the end of experiment (day 42). Diet FE had a lower pH than diet RDR, contained more organic acids, had the lowest counts of E. coli and total coliforms (P < 0.05), and the highest counts of lactic acid bacteria (LAB) (P < 0.05). Piglets fed diets FE and RDR had lower pH (P < 0.05), and higher concentrations of organic acids in the stomach, ileum and mid-colon (P < 0.05) than piglets fed diet CO. Counts of LAB in stomach and ileum were higher in animals fed diets FE and RDR than CO (P < 0.05), while the number of E. coli and total coliforms along the gastrointestinal tract was reduced. The ileal digestibility of crude protein and organic matter was improved in piglets fed diet RDR (P < 0.05) compared with piglets fed diet CO. Piglets fed diet RDR had a higher weight gain (P < 0.05) and a better feed utilization (P < 0.05) than piglets fed the other diets. The present findings support the contention that fermented diets and/or fermented feedstuffs can be used for dietary manipulation to improve gut health and animal performance. 相似文献
The aim of the present study was to investigate preferences of piglets for diets varying in lysine (Lys) concentration and whether those preferences are influenced by changing position of diets on offer in feeders. For this purpose, a feeding trial was carried out over a period of 5 weeks. Piglets (equal proportions of barrows and gilts) with an initial BW of 7.4 ± 0.9 kg were randomly subdivided into four groups of 12 pigs each and were housed in single pens. Two reference groups were fed diets containing either 0.7% Lys (low-Lys group) or 1.0% Lys (high-Lys group). Two other groups had the choice from two diets containing 0.7 or 1.0% Lys offered simultaneously in identical feeders. For animals of one of these choice groups (Lys-choice 1 group) the position of diets in the feeders remained unchanged throughout the experimental period, but for animals of a second choice group (Lys-choice 2 group) position of the diets on offer in the feeders was changed two times a week.Average daily feed intake and daily gain were greatly (p < 0.05) decreased in animals of the low-Lys group compared to other treatments, and feed to gain ratio was increased. Performance of animals in Lys-choice 1 group was numerically decreased compared to animals of Lys-choice 2 group. Chosen diets of Lys-choice groups 1 and 2 contained on average 31 and 19% feed with 0.7% Lys, respectively. Resulting Lys contents of total diets were 0.94 and 0.98% for Lys-choice groups 1 and 2, respectively, as a mean of the experiment. In week 1 animals of both Lys-choice groups met approximately 50% of total feed intake by consumption of the 0.7% Lys diet. Animals of Lys-choice 1 group lowered this portion to 20% in the last experimental week and animals of Lys-choice group 2 to 11%. Preference for the 1.0% Lys diet was evident (p < 0.05) from week 3 to 5 in Lys-choice 1 group and from week 2 to 5 in Lys-choice 2 group.Piglets are able to distinguish between diets differing in Lys content and to prefer a better balanced diet over a Lys-deficient one. Changing the position of the diets on offer in the feeders two times a week does not impair the ability to select for a diet more adequate in Lys concentration, but led in contrary to a slightly higher preference for Lys. 相似文献
The present study was designed to investigate the effect of dietary fish oil supplementation on piglet T helper cells (Th) polarization in relation to its impact on piglet serum interferon γ (IFN-γ) and interleukin 10 (IL-10) concentrations and splenic expression of Th1/Th2 characteristic genes. The diets of 18 gestating sows were supplemented with 7% lard (C) (n = 10) or 7% fish oil (T) (n = 8) from 10 d before parturition to weaning. At weaning, a split plot experiment was designed, 56 piglets, 28 each from sows fed with fish oil diet or lard diet, were divided into four groups of 7 replicates (one female and one castrated male per replicate) based on both sow diet during lactation and post-weaning piglet diet (C had 7% lard and T had 7% fish oil): CC, CT, TC, TT, and were fed the 7% fish oil or lard diet from day 35 to day 70. Serum concentrations of IFN-γ and IL-10, and Th1/Th2 related genes expression levels in spleen were measured and analyzed. The results showed that piglets fed with fish oil diet during post-weaning tended to have higher serum IFN-γ/IL-10 ratio (P = 0.09) than lard diet fed piglets. Lactation fish oil feeding increased splenic IL-12b, IL-12 receptor β2 (IL-12Rβ2), IL-2 and IFN-γ genes expression (P < 0.05 or P < 0.01) and post-weaning fish oil feeding increased splenic IL-12b (P = 0.06), IL-2 (P < 0.01) and IFN-γ (P = 0.08) mRNA expression than that in lard diet fed piglets at the end of this experiment. On the other hand, IL-4 gene expression (P = 0.01) in spleen was lower in weaned piglet from fish oil diet fed sows than that from lard diet fed sows. However, post-weaning piglets fed fish oil diet had higher splenic IL-4 (P = 0.06), IL-6 (P < 0.01) and IL-10 (P = 0.05) mRNA abundances than that fed with lard diet. These results indicated that dietary fish oil during lactation could increase Th1 polarization and accelerate immune maturation; while 7% fish oil in weaned piglets' diet was likely to increase Th2 cytokines expression. 相似文献
A study was carried out to investigate whether adding a starter culture or a formic acid containing-product to fermented liquid feed of suboptimal microbial quality could improve the characteristics of the mixture and the gastrointestinal ecology and growth performance of piglets. Four experimental diets were prepared: Feed and water were mixed in the ratio 1:2.5 (w/w) in four closed tanks. Diet 1) Three times daily, 50% of the mixture stored in the tank was removed and then replaced with an equal amount of fresh feed and water, (FLF), positive control; Diet 2) Three times daily, 95% of the mixture stored in the tank was removed and then replaced with an equal amount of fresh feed and water. Escherichia coli K12 (1 × 106/g) was added every morning (COLI); Diet 3) as the COLI treatment and added Lactobacillus plantarum VTT E-78076 (1 × 106/g) every morning (+PLANTARUM); and Diet 4) as the COLI treatment and added 0.26 g AIV/KemiSile 2 plus/100 g mixture three times daily (+ACID). One hundred and sixty weaners were used. On day 14, one littermate from each pen was killed and the gastrointestinal tract removed. The remaining piglets were used to measure the effect of the experimental diets on growth performance during the first six weeks post-weaning. The +ACID diet had lower numbers of lactic acid bacteria and Enterobacteriaceae, higher numbers of yeasts, and lower concentration of lactic acid and acetic acid than the COLI and +PLANTARUM diets. Feeding the +PLANTARUM and the +ACID diets increased daily weight gain during the six weeks post-weaning (P ≤ 0.02) compared to feeding the COLI diet. The +ACID group showed the highest numerical daily feed intake in all periods. The gain/feed during the six weeks post-weaning tended to be lowest in the COLI group (P < 0.10). The counts of lactic acid bacteria able to grow at 20 °C were lowest in the stomach (P ≤ 0.003) and distal small intestine (P ≤ 0.06) of the +ACID group. The counts of yeasts able to grow at 37 °C in the stomach were highest in the +ACID (P ≤ 0.01) and FLF groups (P ≤ 0.09). The number of yeasts able to grow at 20 °C in the stomach and distal small intestine was highest in the FLF fed piglets, followed by the +ACID group. This study showed that addition of L. plantarum VTT E-78076 or AIV/KemiSile 2 plus to fermented liquid feed of suboptimal microbial quality improved growth performance without affecting the gastrointestinal ecology of piglets substantially. 相似文献
Two experiments were conducted to measure the apparent ileal digestibility coefficients (AID) of protein and amino acids from canola meal (CM) and a pelleted canola meal (PCM) and their effect on specific activity (SA) of pancreatic proteases in weaned piglets and growing pigs. In experiment one, 24 piglets weaned at 17 days and weighing 5.5 kg were used. Treatments were a reference diet with 200 g of crude protein (CP) kg− 1 elaborated with casein (C) as the sole protein source, a diet containing C–CM and a diet containing C–PCM. These diets were obtained using the reference diet plus 100 g kg− 1 of CM or PCM that substituted an isoproteic mixture of casein and maize starch from reference diet, so that the AID coefficients for their protein and amino acids could be calculated by difference. In experiment two, nine castrated pigs weighing 39.5 kg were used. Treatments were a reference diet with 160 g of CP kg− 1 elaborated with casein (C) as the sole protein source, diet C–CM and diet C–PCM. These diets were obtained using the reference diet plus 300 g kg− 1 of CM or PCM that substituted an isoproteic mixture of casein and maize starch from reference diet. In piglets, the AID coefficients for casein were highest (P < 0.05), those of PCM were intermediate, and those of CM were the lowest. In older pigs, the AID coefficients for casein were highest, and those of CM and PCM were similar (P > 0.05). The SAs of chymotrypsin, trypsin and carboxypeptidases A and B were lower in piglets than in heavier pigs. Moreover, the SAs of trypsin, chymotrypsin, and carboxypeptidase B were lower (P > 0.05) in animals fed casein. The results showed in piglets that whereas CM was less digestible, pelleted canola meal improved protein and amino acid ileal digestibility, resulting in similar AID coefficients to those of growing pigs. 相似文献