Effect of 2, 3-butanedione monoxime (BDM) on myocardial protection in cold cardioplegia

AIM: The effects of BDM on isolated rat heart in cold cardioplegia were studied. METHODS: Rat heart were subjected to cold cardioplegia at 4℃ for 8, 18 and 24 h.Then each heart was perfused (90 cm H₂O) in Langendorff model at 37℃ for 40 min. In the high K⁺ group(n=24) the hearts were preserved in St.Thomas cardioplegic solution, in BDM group(n=24) hearts were preserved in K-H solution with BDM 30 mmoL/L. RESULTS: After 18 h, heart rate and the coronary flow in BDM group were significantly higher than in high K⁺ group(P＜0.05). Activity of Na⁺-K⁺-ATPase in cell membrane and in mitochondrial membrane in the BDM group was significantly higher than high K⁺ group(P＜0.01). After 24 h, all hearts in high K⁺ group were dead, but were alive in BDM group. CONCLUSION: Under given experiment conditions, BDM did enhance the tolerance to cold ischemia significantly. The results showed that BDM may become a useful agent for prolong the storage period of heart in cold cardioglegia.     

杂交水稻区域试验非平衡资料产量性状的分析

对１９９１－１９９２年全国籼型杂交水稻区域试验中１５个迟熟组新组合在２２个参数试点的汇总资料，运用朱军等提出的作物品种区域试验非平衡资料的统计分析方法，进行了产量性状的稳定性分析，并选择了不同地区选送的组织进行线性对比。     

杀虫剂防治蔬菜斑潜蝇不同调查方法的评价

本文对杀虫剂防治蔬菜斑潜蝇田间药效试验的多种调查方法进行了试验比较 ,认为在试验过程中将蛹做为活虫数计算 ,同时记录植株新生叶片及未被害叶片在药后出现的试虫数 ,能够全面正确反映农药的作用效果。在随机摘叶调查时 ,认为应即时检查 ,该方法所采集叶片不宜太少 ,较适合于小叶作物     

New gene-based approaches for an AIDS vaccine

Vaccine approaches against AIDS have focused on inducing cellular immune responses, since many studies revealed the role of T cell responses in the control of human immunodeficiency virus or simian immunodeficiency virus (SIV) infections. The experimental infection of rhesus macaques with SIV or chimeric SHIV is routinely used as a model for AIDS. In such models, DNA immunization is a tool to elicit specific T cell responses and to study their protective efficacy. DNA immunogenicity in primates depends on parameters such as level of antigen expression, choice of the antigen among SIV proteins, use of fusion proteins, route of immunization, and addition of adjuvants. Recent results suggest that priming with DNA and boosting with attenuated recombinant viral vectors, each expressing corresponding SIV antigens, leads to improved specific immunity and, in some cases, affords protection against pathogenic challenge. After preclinical evaluations, DNA has entered clinical trials for a therapeutic or prophylactic gene-based AIDS vaccine.     

云南中亚热带禾本科高产饲草引种试验

在云南中亚热带试验点,对8个禾本科饲草栽培种进行引种栽培试验,结果表明:墨西哥类玉米、8493墨西哥类玉米、大力士甜高粱、先锋高丹草和M-8IE甜高粱5个栽培种干物质产量高,适应性强,适宜在相似气候条件地区推广种植。     

紫花苜蓿品种比较试验

通过对26个紫花苜蓿（Medicago sativa）品种的生育期、株高、产量、越冬率等指标的试验比较，结果表明：公农一号、保丰、陇东、德福等几个品种的表现优于其他参试品种，适宜在辽西北风沙地地区推广种植。     

肉鸡肠道PepT1 mRNA表达的肠段差异性与发育性变化

选用遗传背景相同的1日龄父母代雄性Arbor Acre(AA)鸡和父母代雄性岭南黄肉雏鸡各120羽,采用相对定量RT-PCR方法,以30 d时岭南黄鸡肠道样品为模板,研究肉鸡肠道寡肽转运载体1(Peptide transporter 1,PepT1)mRNA表达的肠段差异性;以AA肉鸡和岭南黄鸡十二指肠和空肠样品为模板,研究不同品种肉鸡肠道PepT1 mRNA表达的发育性变化。结果显示:(1)岭南黄肉鸡肠道PepT1 mRNA的表达丰度从十二指肠、空肠、回肠到结直肠依次降低,其中十二指肠显著高于结直肠(P<0.05);(2)AA鸡和岭南黄肉鸡PepT1 mRNA在十二指肠及空肠中的表达具有相同的发育模式,16 d表达丰度最高,16~44 d逐渐下降,58 d略微回升;不同基因型之间PepT1 mRNA丰度比较,AA鸡和岭南黄肉鸡两品种间PepT1 mRNA的表达没有显著差异(P>0.05)。以上结果说明:(1)随着肠道空间位置的后移,岭南黄肉鸡肠道PepT1 mRNA的表达丰度逐渐降低,其中十二指肠的表达丰度显著高于结直肠(P<0.05);(2)不同品种肉鸡十二指肠及空肠PepT1 mRNA的表达具有相同的发育模式,且同日龄两品种间的表达丰度未见明显差异,表明PepT1 mRNA表达受到发育阶段的调控,但品种间具有稳定性。     

公鸡和母鸡氮代谢及氮维持需要量的比较研究

试验采用氮平衡试验研究产蛋高峰期罗曼公、母鸡的氮维持需要量(Nm),并分析公、母鸡氮代谢的变化规律。试验选取27周龄健康的公、母鸡各30只,分别分为5个处理,每处理6个重复,每个重复1只试鸡,对公、母鸡分别饲喂5个不同粗蛋白质水平的试验日粮。结果表明:相同蛋白水平处理组的公鸡氮食入量和氮沉积率均高于母鸡的结果。母鸡和公鸡的氮食入量(NI)与氮沉积量(NB)间的回归方程分别为NB=0.3743NI-0.1589(R2=0.79)和NB=0.6228NI-0.3151(R2=0.85)。母鸡和公鸡的氮维持需要量分别为每天0.4245g和0.5059g;每千克体重0.2364g和0.2754g;每千克代谢体重0.2739g和0.3208g。     

陕北黄土高原生态资产与区域生态安全程度研究

在GIS支持下,通过测定陕北黄土高原植被生产和生态调节的物质量及生态资产价值.结果表明:①通过计算,陕北黄土高原每年的生态资产总价值为7.00×1010元,其中释放氧气的价值最高,占总价值的29.51%;土壤保持价值最小,占总价值的1.5%.而就植被类型而言,果树的生态价值最高,占总价值的43.89%;最小是沼泽,占总价值的0.4%.②通过地理信息系统与生态学的结合,对陕北黄土高原的生态资产进行定量测量,并利用GIS软件的空间分析功能,绘制了陕北黄土高原生态资产价值空间分布图.③陕北黄土高原生态安全程度为0.45,处于生态不安全状态,负荷超载,要狠抓生态建设.④针对陕北黄土高原环境现状,提出相应的治理对策.     

青海河湟地区水资源综合开发与区域经济发展研究

本文分析了河湟地区水资源特征、开发利用现状及存在的问题,在此基础上,论述了河湟地区水资源开发利用与区域经济发展模式。研究表明:加快开发黄河水能资源,建立以水能资源开采业为主导的产业群发展;浅山地区因地制宜推广雨水集流利用工程,走出“水窑+科技+节水产业+生态”的发展模式;“引大济湟”工程有助于提高水资源可利用量,推进湟水流域全面发展。