全文获取类型
收费全文 | 2855篇 |
免费 | 147篇 |
国内免费 | 374篇 |
专业分类
林业 | 227篇 |
农学 | 228篇 |
基础科学 | 571篇 |
410篇 | |
综合类 | 1044篇 |
农作物 | 185篇 |
水产渔业 | 70篇 |
畜牧兽医 | 503篇 |
园艺 | 61篇 |
植物保护 | 77篇 |
出版年
2024年 | 15篇 |
2023年 | 54篇 |
2022年 | 109篇 |
2021年 | 126篇 |
2020年 | 115篇 |
2019年 | 150篇 |
2018年 | 78篇 |
2017年 | 151篇 |
2016年 | 190篇 |
2015年 | 141篇 |
2014年 | 163篇 |
2013年 | 135篇 |
2012年 | 224篇 |
2011年 | 181篇 |
2010年 | 165篇 |
2009年 | 185篇 |
2008年 | 138篇 |
2007年 | 185篇 |
2006年 | 142篇 |
2005年 | 124篇 |
2004年 | 88篇 |
2003年 | 83篇 |
2002年 | 63篇 |
2001年 | 60篇 |
2000年 | 52篇 |
1999年 | 42篇 |
1998年 | 33篇 |
1997年 | 28篇 |
1996年 | 25篇 |
1995年 | 25篇 |
1994年 | 18篇 |
1993年 | 20篇 |
1992年 | 14篇 |
1991年 | 12篇 |
1990年 | 8篇 |
1989年 | 8篇 |
1988年 | 6篇 |
1987年 | 7篇 |
1986年 | 4篇 |
1984年 | 1篇 |
1983年 | 2篇 |
1980年 | 2篇 |
1978年 | 1篇 |
1976年 | 3篇 |
排序方式: 共有3376条查询结果,搜索用时 31 毫秒
101.
Carolyn J. Broccardo Gisela Soboll Hussey Lutz Goehring Paul Lunn Jessica E. Prenni 《Journal of Equine Veterinary Science》2014
Cerebrospinal fluid (CSF) is a biofluid that is reflective of overall health. Although proteomic profiling of human CSF has been performed in the context of a variety of disease states, this report represents the first comprehensive proteomic analysis of equine CSF. A total of 320 proteins were confidently identified across six healthy horses, and these proteins were further characterized by gene ontology terms mapped in UniProt, and normalized spectral abundance factors were calculated as a measure of relative abundance. Theses results provide an optimized protocol for analysis of equine CSF and lay the groundwork for future studies involving the study of equine CSF in the context of pathogenic disease states. 相似文献
102.
103.
以黄化番茄和普通番茄幼苗为试材,研究UV-B辐射对番茄幼苗生长和光合作用的影响.结果表明:UVB辐射处理对番茄幼苗株高、茎粗、叶片的抑制作用非常明显,且对叶绿素破坏严重,导致幼苗光合速率降低,类黄酮含量升高10%以上.UV-B处理后两种番茄生长都受到明显抑制,但以黄化苗的变化更为明显,表明黄化番茄幼苗对UV-B辐射更为敏感. 相似文献
104.
The occurrence of Flavobacterium psychrophilum at four rainbow trout hatcheries was investigated to provide more knowledge about the reservoirs and transmission of this bacterium. Broodstock were sampled at stripping (including both unfertilized and fertilized eggs), and the offspring were then sampled at the eyed egg and fry stages. Water and surface samples (e.g. hatchery trays) were also sampled. Flavobacterium psychrophilum was found in ovarian fluid and milt, indicating that broodstock may serve as a reservoir and are latent carriers of the pathogen. Flavobacterium psychrophilum was not found on or inside eggs, but further egg studies will be necessary to elucidate the possibility of vertical transmission of the pathogen. Flavobacterium psychrophilum was isolated from water samples, but only from water that had been in close contact with farmed rainbow trout or eggs. Flavobacterium psychrophilum isolates were characterized and compared with well-characterized strains, using degradation of elastin, serotype and ribotype profiles. Different ribotypes of F. psychrophilum were found between hatcheries, but a common ribotype A was found at all four hatcheries. Different ribotypes were found in broodstock without clinical disease, whereas only a few ribotypes (mostly ribotype A) were found in diseased fry. The same ribotype A was found in broodstock, in water samples from hatchery trays and in fry, which suggests the possibility of transmission of F. psychrophilum between broodstock and offspring. 相似文献
105.
106.
The present study investigated the relationships between egg viability and ovarian fluid composition, egg physiology and egg metabolism in lake trout, Salmo trutta lacustris, to obtain biomarkers for egg quality determination. The ovarian fluid pH, protein levels and activities of aspartate aminotransferase and -d-glucuronidase were significantly correlated with egg viability expressed as the number of eyed stage embryos. Regression models demonstrated that an ovarian fluid pH between 8.44 and 8.57, protein levels below 235.56 mg 100 ml–1ovarian fluid, aspartate aminotransferase activity below 31.65 m min–1 l–1ovarian fluid and -d-glucuronidase activity below 8.62 m min–1 l–1 ovarian fluid characterized egg batches with high viability (80%).The increase in the egg wet weight during water hardening was also significantly correlated with the number of eyed stage embryos, and egg batches with high egg viability (80%) increased in wet weight by 13% during water hardening.From the investigated metabolic parameters the number of eyed stage embryos was significantly correlated with activities of NADP-dependent isocitrate dehydrogenase (egg viability 80% at 2.07 nM min–1 mg–1 protein) and NAD-dependent malate dehydrogenase (egg viability 80% at 47.25 nM min–1 mg–1 protein), with the respiration rate (egg viability 80% at 8.71 nM min–1 mg–1 protein), with the ratio of NADH to NAD levels (egg viability 80% 0.872), with the levels of free, non-esterified fatty acids (egg viability 80% 72.34 g mg–1 protein), and the ratio of non esterified to esterified fatty acids (egg viability 80% at 0.749). Also, subjective and visual control methods were described to distinguish between batches with viable and non viable eggs. 相似文献
107.
G.?J.?Dietrich R.?Kowalski M.?Wojtczak S.?Dobosz K.?Goryczko A.?CiereszkoEmail author 《Fish physiology and biochemistry》2005,31(1):1-9
The present study investigated the effects of sequential collection of milt, time of post-mortem storage and anesthesia on rainbow trout (Oncorhynchus mykiss) sperm motility parameters (using computer-assisted sperm analysis – CASA) as well as seminal plasma osmolality and sperm
concentration. The post-mortem storage and time of anesthesia altered motility characteristics of rainbow trout sperm to different extents. The moderate
impact of time of anesthesia was manifested in a shortened duration of sperm motility after 10 min exposure of fish to anesthetic.
The prolonged post-mortem storage (≥40–60 min), in addition to lowering sperm motility duration, also significantly influenced sperm motility parameters,
such as sperm velocities, percentage of motile sperm and sperm trajectory parameters. These results clearly demonstrate that
when milt from sacrificed fish is used for sperm motility studies, the time of post-mortem storage significantly alters sperm motility characteristics. Since sperm motility rate and swimming velocity could predict
fertilizing ability, detrimental effects of prolonged post-mortem storage may lead to reduced fertilization success. Sperm concentration and seminal plasma osmolality were lower in the first
fractions and increased with successive collections of milt. It suggests the presence of urine contamination of the first
milt fractions which were collected by stripping. Therefore, testing of sperm concentration and/or seminal plasma osmolality
should be mandatory while handling stored milt. 相似文献
108.
选择宜优673、泰丰优656、中浙优1号、泸优明占等13个优质稻品种作烟后稻进行不同播期处理,采用自然低温胁迫的方法,观察优质稻对低温的反应及水稻产量构成因素的变化,以期筛选出抗逆性强的品种。 相似文献
109.
为探讨机械直播稻田稗最佳防控时间,采用小区试验的方法,以人工除草处理为对照,研究稗不同出苗时间(水稻机械直播后0、7、14、21、28、35 d)和不同密度(1、3、5、7株·m-2)对机械直播水稻根系特性以及产量的影响。结果表明,稗出苗时间和密度对水稻产量有不同的影响,稗草出苗时间越早或密度越大,水稻减产越多。当稗密度为1株·m-2时,水稻机械直播后0~35 d出苗的稗均对水稻产量无影响;当稗密度为3、5、7株·m-2时,在水稻机械直播后21 d内出苗的稗均随其密度增加而显著降低水稻产量,之后出苗的稗对水稻产量无明显影响。同时发现,产量显著降低后水稻的根系总根长、平均根系直径、根干重、根系总吸收面积、根系活跃吸收面积、根系氧化力和玉米素+玉米素核苷含量也均显著降低。上述水稻根系指标的降低可能是机械直播水稻与稗伴生后产量降低的重要原因。本研究为机械直播稻田稗草防控提供了理论依据。 相似文献
110.
液力偶合器三维涡识别方法及流场时空演化 总被引:1,自引:1,他引:0
精细刻画液力偶合器内部非定常多尺度三维涡结构对于揭示液力偶合器流场时空演化规律与能量损耗机理具有重要意义。该研究基于计算流体动力学理论,采用应力混合涡湍流模型多尺度解析模拟制动工况下液力偶合器三维旋涡流场。通过3种不同的涡识别方法提取涡轮内部多尺度涡系结构,从空间重构效果、阈值选择范围及敏感性角度分析不同涡识别方法的适用性。依托粒子图像测速(Particle Image Velocimetry,PIV)流场试验验证数值模拟及涡结构辨识结果的准确性与可靠性。围绕三维涡结构特征解析结果和二维流场图谱信息,分析并揭示流道内部湍流的时空演化规律及能量损耗机理。结果表明:Q准则方法的阈值选择盲目性大,难以同时识别强涡与弱涡结构,丢失很多涡结构细节特征,无法准确预测三维涡空间连续性运动趋势;Ω方法能够在阈值范围为0.51~0.59内精确辨识弱涡结构,但涡系空间重构后涡的运动趋势感不强;ΩL方法对阈值不敏感,在阈值范围为0.51~0.67内该方法空间涡系重构效果最好,强涡、弱涡结构特征识别度高。制动工况下涡轮内部整体流动为逆时针大尺度环流,并伴有小尺度涡流等局部流动现象,与主流涡运动相同旋向的旋涡促进主流旋涡运动,相反旋向的旋涡阻碍主流旋涡运动,由于涡的撕裂、破碎、分离、碰撞、摩擦、挤压等作用,导致流体能量损失,以热能形式耗散。研究结果可为液力偶合器结构设计与优化提供理论与技术指导。 相似文献