Motility Parameters of Rainbow Trout (<Emphasis Type="Italic">Oncorhynchus mykiss</Emphasis>) Spermatozoa in Relation to Sequential Collection of Milt,Time of <Emphasis Type="Italic">Post-mortem</Emphasis> Storage and Anesthesia |
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Authors: | G?J?Dietrich R?Kowalski M?Wojtczak S?Dobosz K?Goryczko Email author" target="_blank">A?CiereszkoEmail author |
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Institution: | (1) Institute of Animal Reproduction and Food Research, Polish Academy of Science, Tuwima 10, 10-747 Olsztyn, Poland;(2) Department of Salmonid Research, Inland Fisheries Institute, Rutki, 83-330 Żukowo, Poland;(3) Chair of Fish Biology and Pisciculture, University of Warmia and Mazury, Oczapowskiego 5, 10-718 Olsztyn, Poland |
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Abstract: | The present study investigated the effects of sequential collection of milt, time of post-mortem storage and anesthesia on rainbow trout (Oncorhynchus mykiss) sperm motility parameters (using computer-assisted sperm analysis – CASA) as well as seminal plasma osmolality and sperm
concentration. The post-mortem storage and time of anesthesia altered motility characteristics of rainbow trout sperm to different extents. The moderate
impact of time of anesthesia was manifested in a shortened duration of sperm motility after 10 min exposure of fish to anesthetic.
The prolonged post-mortem storage (≥40–60 min), in addition to lowering sperm motility duration, also significantly influenced sperm motility parameters,
such as sperm velocities, percentage of motile sperm and sperm trajectory parameters. These results clearly demonstrate that
when milt from sacrificed fish is used for sperm motility studies, the time of post-mortem storage significantly alters sperm motility characteristics. Since sperm motility rate and swimming velocity could predict
fertilizing ability, detrimental effects of prolonged post-mortem storage may lead to reduced fertilization success. Sperm concentration and seminal plasma osmolality were lower in the first
fractions and increased with successive collections of milt. It suggests the presence of urine contamination of the first
milt fractions which were collected by stripping. Therefore, testing of sperm concentration and/or seminal plasma osmolality
should be mandatory while handling stored milt. |
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Keywords: | anesthesia milt collection ovarian fluid post-mortem storage rainbow trout sperm motility |
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