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81.
为建立HPLC法测定牛奶中磺胺类药残留量的方法。样品经乙腈处理沉淀蛋白质后取上清液浓缩,以乙腈-水(体积比为1∶1)为流动相进行高效液相色谱分析。结果表明4种磺胺类药分离效果良好,平均回收率范围81.6%~95.8%,相对标准偏差为1.5%~4.2%,最低检出限达1.0~2.0ng/mL。可见该检测法具有灵敏、准确、精密、无杂质干扰等优点。 相似文献
82.
龙葵制剂中澳洲茄胺盐酸盐含量测定 总被引:1,自引:0,他引:1
建立了高效液相色谱法(HPLC)测定澳洲茄胺盐酸盐含量的方法.色谱柱为Zorbax Extend-C18(250mm×4.5mm,5μm),以乙腈-甲醇-乙酸铵为流动相(体积比为60∶ 35∶ 5),UV检测器(204nm),在室温下测定澳洲茄胺盐酸盐含量.澳洲茄胺盐酸盐的质量浓度范围在0.8~160mg·L-1时(n≥5000),检测信号与质量浓度线性关系良好Y=166X-1.72×10-14(r=1.000);样品平均回收率(n=9)分别为98.6%.此法简单、重复性好,可用于澳洲茄胺盐酸盐制剂的含量测定. 相似文献
83.
In this work, the contents of oil, sugar and organic acids of olive fruit samples (Domat, Memecik and Uslu) were determined by using HPLC at different maturation stages. Olive fruit samples presented a common organic acid profile, composed of four constituents: oxalic, citric, malic and succunic acids. Total organic acid content of Domat variety was found to be 4787.1 ± 4.53 mg/100 g in January. Memecik variety had the highest value at the beginning of the ripening period (6385.08 ± 4.90 mg/100 g). The amount of organic acid in Uslu variety reached up to the value of 10942.5 ± 30.41 mg/100 g. The results were evaluated statistically to determine relationship between the oil, sugar and organic acid content in three olive varieties during maturation. A positive correlation was found between the total sugar and organic acid (r = 0.54, p < 0.01) whereas a low positive correlation was observed between the contents of oil and total organic acids (r = 0.46, p < 0.05) in olive varieties. 相似文献
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87.
淡水鱼肝脏中维生素A1,A2含量的研究 总被引:5,自引:0,他引:5
本试验建立了用高效液相色谱法同时测定淡水鱼肝脏中的VA1和VA2的方法。以μ-Porasil3.9nm×150mm(Waters Co.)为色谱柱,正己烷:乙醚(87:13)为流动相,紫外350nm,荧光Ex325nm,Em480nm双道检测,VA1和VA2的保留时间分别为26.25min和28.00min。采用不同波长条件下的紫外吸收特性,对VA2予以定性,以VA1为内标物对VA2予以定量。实验 相似文献
88.
Seven wild and ten cultivated blackberries (Arapaho, Bartin, Black Satin, Bursa 1, Bursa 2, Cherokee, Chester, Jumbo, Navaho, and Ness), and six lowbush (Vaccinium arctostaphylos) and four highbush (Vaccinium corymbosum) blueberries fruits (Ivanhoe, Jersey, Northland, and Rekord) were analyzed for total anthocyanins, total phenolics, and antioxidant activity as ferric reducing antioxidant power (FRAP) in this study. The respective ranges of total anthocyanin and total phenolic contents of the tested samples were: blackberries, 0.95–1.97 and 1.73–3.79 mg g−1 and blueberries, 0.18–2.94 and 0.77–5.42 mg g−1. FRAP values varied from 35.05 to 70.41 μmol g−1 for blackberries, 7.41 to 57.92 μmol g−1 for blueberries. Wild blackberries had the highest FRAP values while wild blueberries had the highest total phenolic and total anthocyanin contents. A linear relationship was observed between FRAP values and total phenolics for blueberries (r = 0.981). The anthocyanin pigments in samples were isolated and characterized by high-performance liquid chromatography (HPLC) with UV–visible detection. Cyn-3-glu was the predominant anthocyanin in all blackberry fruits. 相似文献
89.
蛹草拟青霉固体发酵条件优化 总被引:1,自引:0,他引:1
为了提高蛹草拟青霉cmily-02产虫草素得率,采用正交实验优化固体发酵培养基配方。以虫草素产量为指标,通过高效液相色谱法检测,筛选出最优固体培养基为:大米粉2.0%、青稞粉0.5%、玉米粉3.0%、黄豆粉0.5%、粗麦麸30%、KH2PO3 0.05%、MgSO4 0.1%。从培养温度和干燥温度2方面,探讨了温度对虫草素产量的影响,得出产虫草素的最佳培养温度为28℃,最佳干燥温度为30℃。通过固体发酵条件优化,虫草素产量可达到1 850μg.g-1。 相似文献
90.
The gland cells were successfully collected from the stems of Lysimachia fordiana Oliver, and the homologous pigments of fordianin A, fordianin B, fordianaquinone A and fordianaquinone B were firstly detected in the glands by HPLC. This indicated that the stem was an ideal material for the preparation of the glands, and the gland was a center for the polycyclic pigments accumulation in this species. 相似文献