RNAi沉默HvFTZ-F1对茄二十八星瓢虫幼虫存活及发育的影响

茄二十八星瓢虫Henosepilachna vigintioctopunctata是茄科植物上的一种重要害虫,对我国的作物生产造成了严重的经济损失。RNAi（RNA inteference）被认为是防控这种害虫的一种新策略。FTZ-F1是昆虫生长发育过程中重要的调控基因。本研究通过饲喂茄二十八星瓢虫1龄幼虫5 ng/μL dsHvFTZ-F1处理过的茄子叶片,发现幼虫中HvFTZ-F1表达水平显著降低了63.69%,抑制1龄幼虫蜕皮进入2龄,致死率高达65.00%。同时,沉默HvFTZ-F1使得20E相关基因（HvDIB、HvSPOOK、HvSHADOW、HvSHADE、HvE75）表达水平分别降低了85.36%、66.32%、52.74%、31.12%和20.76%;色素沉积相关基因（HvDDC、HvTH、Hvebony）表达水平分别降低了88.74%、91.97%、86.15%;20E滴度和多巴胺滴度分别降低了15.97%和21.38%。综上,HvFTZ-F1在茄二十八星瓢虫的存活和生长发育中起重要作用。HvFTZ-F1可作为一种高效靶标基因用于开发基于RNAi技术的RNA生物农药,用于茄二十八星瓢虫的生物防治。     

液相色谱-串联质谱法检测猪肉中20种违禁药物残留的研究

建立了猪肉中违禁药物（糖皮质激素、性激素、β2-肾上腺素受体激动剂、玉米赤霉烯酮4类）同时测定的高效液相色谱-串联质谱方法。试样经盐酸水解,乙酸乙酯提取,低温冷冻去除脂类,亲水亲脂平衡固相萃取柱净化。采用C18反相色谱柱分离,以乙腈+水为流动相梯度洗脱,分别在电喷雾离子源正、负离子模式下以多反应监测模式（MRM）分段扫描同时检测,基质匹配内标法定量。结果表明,20种违禁药物在相应的浓度范围内线性良好,相关系数均大于0.99;该方法在猪肉中的检测限（LOD,S/N=3）和定量限（LOQ,S/N=10）分别为0.5、1.0 μg/kg。在0.5~10 μg/kg添加浓度水平下,平均回收率为63.9%~117%,批内变异系数1.9%~9.1%,批间变异系数4.9%~13.1%。该方法简单、快速、准确,适用于猪肉中多类违禁药物的残留检测。     

树形对桃树生长、产量和品质的影响

[目的]探讨中油20号桃不同树形对树体生长、产量和品质的影响.[方法]研究3年生中油20号桃Y字形、主干形、三主枝开心形、四主枝开心形及V字形5种树形的夏季结果枝部位光照度,成熟果实品质及冬季树体结构.[结果]同一树形不同部位的光照度和果实品质均有显著性差异,均为上层＞中层＞下层,不同树形相同部位间差异不显著.每666...     

高产抗SCN大豆新品种嫩丰20的选育及高产栽培技术

本文介绍了高产抗SCN大豆新品种嫩丰20的选育经过、主要特征特性及栽培技术.嫩丰20原代号为嫩9702-2,是黑龙江省农业科学院齐齐哈尔分院以合丰25为母本,安8711-277为父本进行有性杂交,系谱选育而成.2008年通过黑龙江省农作物品种审定委员会审定命名推广.     

PttGA20-氧化酶基因dsRNA抑止载体的构建(英文)

[Objective] Genetic Engineering technology was used to regulate the expression of PttGA20-oxidase gene thus restrained plant height growth and internode elongation for cultivating dwarfed plant.[Method] Based on the RNAi principle,the gene specific sequences of PttGA20-oxidase in the antisense and sense orientations interrupted by a gene sequence from GUS were cloned into a binary vector pBI121.The selection marker gene npt Ⅱ was replaced with bar gene to RNAi plasmid.[Result] After undergone different endonuclease restrictions,the constructed constraint vector released different segments whose sizes were similar to that of target segment,which demonstrated that the RNAi plasmid of PttGA20-oxidase gene was successfully constructed.[Conclusion] The experiment provided a new way for culturing dwarfed plant.     

塑料大棚温度检测系统的设计

设计了一种基于数字温度传感器DS18820和单片机的温度检测系统.该温度检测系统具有测温范围宽、精度高、控制简单、简单实用,能对环境温度进行实时测量等优点,适用于一般的工农业场合.如塑料大棚温度检测系统等.     

8%黄腐酸与20%福克种衣剂复配对玉米种子活力的影响

[目的]探讨8%黄腐酸与20%福克种衣剂复配对玉米种子活力的影响。[方法]以农大108、郑单17、郑单958、安玉5号和洛玉1号5个玉米品种为试材,将8%黄腐酸与20%福克种衣剂按1∶1的比例复配后,按药种比1∶50对玉米种子进行包衣,采用室内发芽试验,研究复配后的种衣剂对玉米种子活力的影响。[结果]供试的5个玉米品种经复配种衣剂包衣后,与未包衣原品种对照相比,发芽率、发芽值和发芽指数均有不同程度的提高,分别提高了3.4%~5.9%、19.71%~27.86%和9.27%~16.45%。[结论]8%黄腐酸与20%福克种衣剂复配的种衣剂可提高玉米种子的发芽率、发芽值和发芽指数,从而提高玉米种子的活力。     

外源蛋白定位马铃薯淀粉粒表达载体的构建

1材料与方法 1．1材料马铃薯品系：郑1011。受体菌：大肠杆菌BL21。载体质粒pCambia1305—1购自TaKaRa公司;反转录试剂、TaqDNA聚合酶、T4DNA连接酶、限制性内切酶购自TaKaRa公司;DNA回收试剂盒Wizard PCR preps DNA Purification System购自Promega公司。     

A new lupene triterpenetriol and anticholinesterase activity of Salvia sclareoides

A new lupene triterpenetriol was isolated from the acetone extract of the aerial parts of Salvia sclareoides and characterised as (1beta,3beta)-lup-20(29)-ene-1,3,30-triol (1). In addition, nepetidin (2), nepeticin (3), lupendiol (4), (1beta,11alpha)-dihydroxy-lup-20(29)-en-3-one (5), ursolic acid (6), sumaresinolic acid (7) and hederagenin (8), were identified in this Salvia sp. To the best of our knowledge, the compounds 2 and 7 are new constituents in Salvia spp. The acetone, ethanol, butanol and water extracts of the plant were screened for the in vitro inhibitory activity of acetylcholinesterase (AChE) and butyrilcholinesterase (BChE), enzymes which play a role in the Alzheimer disease. All extracts inhibited acetylcholinesterase activity at 10 microg/ml, a remarkable activity since the standard drug rivastigmine does not inhibit acetylcholinesterase at the same concentration. Regarding the butyrilcholinesterase, the acetone extract at 1000 microg/ml was able to inhibit completely the enzyme activity and the butanol and ethanol extracts, at this concentration, produced a potent inhibition of BchE.     

20世纪中国乡村林业变迁初探

论述了20世纪中国乡村林种与树种变迁及其发展热点,探讨了各时期发展乡村林业的异同措施和变迁机理。