| RNAi沉默HvFTZ-F1对茄二十八星瓢虫幼虫存活及发育的影响 |
| |
| 引用本文： | 王亚洁,刘卓琦,李华丽,潘慧鹏,杨春晓.RNAi沉默HvFTZ-F1对茄二十八星瓢虫幼虫存活及发育的影响[J].中国生物防治学报,2022,38(5):1075-1082. |
| |
| 作者姓名： | 王亚洁 刘卓琦 李华丽 潘慧鹏 杨春晓 |
| |
| 作者单位： | 1. 华南农业大学植物保护学院/广东省生物农药创制与应用重点实验室, 广州 510642;2. 华南农业大学亚热带农业生物资源保护与利用国家重点实验室, 广州 510642 |
| |
| 基金项目： | 国家自然科学基金(32272628,31972269) |
| |
| 摘 要： | 茄二十八星瓢虫Henosepilachna vigintioctopunctata是茄科植物上的一种重要害虫，对我国的作物生产造成了严重的经济损失。RNAi（RNA inteference）被认为是防控这种害虫的一种新策略。FTZ-F1是昆虫生长发育过程中重要的调控基因。本研究通过饲喂茄二十八星瓢虫1龄幼虫5 ng/μL dsHvFTZ-F1处理过的茄子叶片，发现幼虫中HvFTZ-F1表达水平显著降低了63.69%，抑制1龄幼虫蜕皮进入2龄，致死率高达65.00%。同时，沉默HvFTZ-F1使得20E相关基因（HvDIB、HvSPOOK、HvSHADOW、HvSHADE、HvE75）表达水平分别降低了85.36%、66.32%、52.74%、31.12%和20.76%；色素沉积相关基因（HvDDC、HvTH、Hvebony）表达水平分别降低了88.74%、91.97%、86.15%；20E滴度和多巴胺滴度分别降低了15.97%和21.38%。综上，HvFTZ-F1在茄二十八星瓢虫的存活和生长发育中起重要作用。HvFTZ-F1可作为一种高效靶标基因用于开发基于RNAi技术的RNA生物农药，用于茄二十八星瓢虫的生物防治。
|
| 关 键 词： | 茄二十八星瓢虫 HvFTZ-F1基因 RNAi 20E滴度 多巴胺滴度 |
| 收稿时间： | 2022-07-13 |
The Effects of Silencing of HvFTZ-F1 via RNAi on the Survival and Development of the 28-Spotted Potato Ladybird,Henosepilachna vigintioctopunctata Larvae |
| |
| WANG Yajie,LIU Zhuoqi,LI Huali,PAN Huipeng,YANG Chunxiao.The Effects of Silencing of HvFTZ-F1 via RNAi on the Survival and Development of the 28-Spotted Potato Ladybird,Henosepilachna vigintioctopunctata Larvae[J].Chinese Journal of Biological Control,2022,38(5):1075-1082. |
| |
| Authors: | WANG Yajie LIU Zhuoqi LI Huali PAN Huipeng YANG Chunxiao |
| |
| Affiliation: | 1. College of Plant Protection, South China Agricultural University/Key Laboratory of Bio-Pesticide Innovation and Application of Guangdong Province, Guangzhou 510642, China;2. South China Agricultural University, State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangzhou 510642, China |
| |
| Abstract: | Henosepilachna vigintioctopunctata is a major pest of Solanaceae plants,causing serious economic losses to crop production in China.RNAi (RNA inteference) has been found as a novel management strategy for controlling this pest.FTZ-F1 is a key regulator of insect growth and development.In this study,by feeding 5 ng/μL dsHvFTZ-F1 solution treated eggplant leaves to 1st instar larvae of H.vigintioctopunctata,the expression level of HvFTZ-F1 was significantly reduced by 63.69%,the larvae were unable to molt normally into the 2nd instar,with the mortality as high as 65.00%.Meanwhile,silencing of HvFTZ-F1 caused the expression levels of 20E related genes,HvDIB,HvSPOOK,HvSHADOW,HvSHADE,and HvE75 was significantly reduced by 85.36%,66.32%,52.74%,31.12%,and 20.76%,respectively;the expression levels of pigmentation-related genes,HvDDC,HvTH,and Hvebony was significantly reduced by 88.74%,91.97%,and 86.15%,respectively;20E titer and dopamine titer decreased by 15.97% and 21.38%,respectively.In summary,HvFTZ-F1 plays an important role in the survival and development of H.vigintioctopunctata.HvFTZ-F1 can be used as an efficient target gene for the development of RNA biopesticide based on RNAi technology for controlling H.vigintioctopunctata. |
| |
| Keywords: | Henosepilachna vigintioctopunctata HvFTZ-F1 RNAi 20E titer dopamine titer |
|
| 点击此处可从《中国生物防治学报》浏览原始摘要信息 |
|
点击此处可从《中国生物防治学报》下载全文 |
|
