菜用睡莲营养成分及重金属含量测定与评价

对绿梗菜用睡莲(Nymphaea tetragona)及红梗菜用睡莲的15营养成分和4种重金属含量进行了测定与评价。结果表明,1与104~140种(品种)新鲜蔬菜已知数据比较,绿梗和红梗菜用睡莲均有较高含量的维生素C、钠及较低含量的干物质、蛋白质、钾、钙、镁、铜、锌、磷及硒;绿梗菜用睡莲有较高含量的粗纤维、锰及较低含量的铁,红梗菜用睡莲有较高含量的铁及较低含量的粗纤维。2菜用睡莲是一种含钠量较高的蔬菜。3绿梗菜用睡莲营养成分含量总体高于红梗菜用睡莲。4湖北产区菜用睡莲的铅、砷、镉及汞等重金属含量符合食品安全国家标准的规定。     

基于高光谱特征参数的冬小麦氮营养指数估算

为了实现快速高精度获取冬小麦氮营养指数的高光谱监测技术，利用美国SVC HR-1024I型野外光谱辐射仪对2017-2019年关中地区的冬小麦进行遥感监测，获取“三边”参数、任意两波段光谱指数和植被指数，通过相关性分析和逐步回归分析方法筛选冬小麦氮营养指数的敏感光谱参数，结合偏最小二乘回归(PLSR)、随机森林算法(RFR)、支持向量机回归(SVR)和梯度增强回归(GBDT)建立冬小麦氮营养指数模型，并对模型估算精度进行验证。结果表明，从拔节期到灌浆期，各时期的氮营养指数与任意两波段光谱指数均呈极显著相关，其中拔节期氮营养指数与任意两波段光谱指数相关性均高于其他时期，且基于一阶导数光谱的归一化光谱指数和比值光谱指数与氮营养指数的相关系数最大，为0.66。拔节期基于梯度增强回归的冬小麦氮营养指数预测模型的决定系数(r²)和均方根误差(RMSE)分别为0.96和0.05,模型验证的r²、RMSE和相对预测偏差(RPD)分别为0.95、0.12和2.12,模型预测精度最高。因此，拔节期基于梯度增强回归的冬小麦氮营养指数估算模型可用于冬小麦氮营养监测...     

Nutrient cycling and biomass flows in a low-quality forest stand improvement system in the Lesser Khingan Range of China

The material flow and bulk internal flow analyses were used to establish a material accumulation and cycling model for a low-quality forest stand improvement system and a series of processes were considered. The model was applied in a one-hectare low-quality forest plot in the Lesser Khingan Range of China. Results showed that during 1997–2007, the stands absorbed 270.19 kg of N, 74.28 kg of P, and 124.39 kg of K from soils, 51.82 kg of N and 2.38 kg of P were directly absorbed by foliage, and 16.25 kg of K was released to soils by eluviation. Until 2007, the accumulated nutrients in the stands included 236.91 kg of N, 65.28 kg of P, and 108.55 kg of K. When horizontal strip clearcutting was applied in 2007, 50% accumulated nutrients in the stands were shifted due to harvesting operations, and 212.74 kg of N, 26.97 kg of P, and 98.88 kg of K were accumulated in soils, declining by 9.47% for N, 3.68% for P, and 17.60% for K, respectively, compared with year 1997. 94.61 t per hectare of biomass was generated, of which the biomass in stands accounted for 87.36%. The felled tree biomass was 36.89 t per hectare, of which 84.90% and 10.03% of biomass were utilized in terms of logs and other means, and the rest was left on site.     

湘西北长果安息香种群数量与分布格局

对湖南石门壶瓶山景关门、桑植龙潭坪茅垭村样地的长果安息香(Changiostyrax raxdolichocarpa)种群数量和分布格局进行调查和分析。结果表明：长果安息香种群的年龄结构呈衰退型，个体数量主要集中在小树(IV龄段)，幼龄级株数和老龄级株数匮乏；长果安息香种群在进入生理死亡年龄前有2个死亡高峰，第1个峰值产生于幼树(II、III龄段)，主要原因是种内和种间竞争(主要是光照)，第2个峰值产生于中树长成大树阶段(V、VI龄段)，除种内、种间对环境的竞争外，生长地土层浅薄、大树倒伏及人为砍伐亦加剧了该峰值的产生；种群整体呈聚集分布类型，小树向中树、大树变化过程表现出聚集分布向随机分布转变，导致长果安息香濒危的主要原因是种子萌发率极低、种群竞争能力较差以及人为干扰等。     

防护条件下梭梭幼苗生长及养分吸收特性

以新疆古尔班通古特沙漠南缘管件防护与传统移栽2种方式移栽的梭梭幼苗为实验材料,通过野外监测与室内分析,探讨了管件防护条件下梭梭幼苗的生长及养分吸收特性。结果表明:整个生育期内,管件防护梭梭幼苗的各生长指标与传统移栽苗相比,增幅最大的为茎粗,其次为株高。年周期内管件防护梭梭幼苗的生物量呈递增趋势,单株生物量累积可达45.25 g·株~(-1);净增加27.10 g·株~(-1),是传统移栽苗的1.38倍(P0.05)。管件防护梭梭幼苗体内的氮素、磷素、钾素含量呈先升高后降低趋势,在同化枝生长期达到峰值,分别为11.376 g·kg~(-1)、1.066 g·kg~(-1)和23.340 g·kg~(-1),较传统移栽苗高24%、15%和8%,差异显著;而其梭梭幼苗氮素、磷素、钾素的总积累量却呈递增趋势,年净增积累量分别为0.297 g·株~(-1)、0.027 g·株~(-1)和0.560 g·株~(-1),是传统移栽苗的2.23倍、1.72倍和1.65倍;各时期梭梭幼苗体内的氮素、磷素、钾素含量及总积累量均高于传统移栽苗。     

自制中药颗粒剂对腹泻仔猪小肠黏膜病理结构及养分消化率的影响

为了研究自制中药复方颗粒剂对腹泻仔猪小肠黏膜组织结构和营养消化率的影响,试验选用人工感染大肠埃希菌引起腹泻的28日龄断奶的杜长大仔猪36头,随机均分为2组,每组3个重复,每个重复6头。抗生素治疗组在饮水中添加盐酸林可霉素2.0 m L/头饮水,2次/d,连续5 d;中药复方颗粒剂治疗组在饮水中添加2.0 g/头饮水,2次/d,连续5 d。分别于用药后14 d时,每组每个重复随机抽取1头屠宰,无菌采集小肠黏膜,通过石蜡切片和HE染色观测小肠黏膜病理组织学变化。其余仔猪继续饲养14 d,采用内源指示剂法进行消化试验。结果显示,小肠黏膜组织结构:中药复方颗粒剂治疗组小肠黏膜除有少量出血外,未发现明显病理变化。抗生素治疗组小肠黏膜脱落,有广泛充血、出血;黏膜上皮细胞变性坏死;嗜酸性粒细胞浸润,有大量以淋巴细胞杯状细胞为主的炎性细胞浸润;微绒毛排列紊乱、脱落;细胞受损严重,线粒体等细胞器结构异常。养分消化率:对于有机物质、磷的消化率,各组之间无显著差异,中药复方颗粒剂治疗组对钙的消化率为57.10%,显著高于抗生素治疗组(56.10%),并与健康仔猪组(57.40%)差异不显著。中药复方颗粒剂治疗组对粗蛋白质的消化率为79.30%,显著高于抗生素治疗组(78.50%),并与健康仔猪组(79.20%)差异不显著。中药复方颗粒剂治疗组对粗纤维的消化率为48.30%,极显著高于抗生素治疗组(46.10%),并与健康仔猪组(48.20%)差异不显著。中药复方颗粒剂治疗组对粗脂肪的消化率为63.40%,极显著高于抗生素治疗组(62.10%),并与健康仔猪组(63.60%)差异不显著。结论:抗生素治疗仔猪腹泻造成小肠黏膜明显的病理变化和功能障碍,而中药治疗有利于仔猪小肠黏膜组织结构的修复,并能显著提高小肠的养分消化率。为防治仔猪腹泻、提高仔猪的生长性能提供依据。     

高粱光敏色素A基因的克隆、蛋白结构与长短日照诱导表达模式

光敏色素是一类红光/远红光受体,对植物光形态建成、外部形态和生理功能等方面起着重要的调节作用。为明晰光敏色素A在调控高粱光形态建成发育过程中的作用,本研究比较分析光温敏感程度不同的高粱品种Btx623、Rio、Tx430和LH645的PhyA基因序列和PHYA蛋白的保守结构域,利用qRT-PCR明确长短日照条件处理下PhyA基因的表达模式。结果表明,Btx623、Rio和Tx430之间PhyA基因序列存在非同义突变,LH645在PhyA基因的第7个外显子上插入了5 bp碱基序列;高粱PhyA蛋白中含有1个PAS-2结构域、1个GAF结构域、1个Phytochrome结构域、2个PAS结构域、1个组氨酸激酶A结构域和1个类似组氨酸激酶的ATP激酶结构;遗传进化树分析表明,单子叶和双子叶植物PhyA蛋白明显聚为2大类,高粱PhyA、谷子PhyA和玉米PhyA1、PhyA2聚为一个亚类,相互间亲缘关系较近,与水稻PhyA同源性相对较远;在短日照(SD)条件下各时期PhyA基因转录水平变化显著,Rio PhyA基因转录水平较Btx623和Tx430变化明显,LH645 PhyA基因转录变化不显著,在长日照(LD)条件下Rio PhyA基因转录水平低于Btx623和Tx430,LH645 PhyA基因转录水平变化不显著。以上结果表明,PhyA受光周期诱导,推测其在高粱光形态建成中起重要调控作用。本研究为进一步解析PhyA基因功能及其在调控高粱光形态建成发育过程中的作用提供理论基础。     

华北地区旅游型村镇住居模式探讨——以保定涞源白石山景区为例

村镇型旅游发展中,旅游者在当地村民家中留宿,村镇的个性化接待和朴素环境让人们赞不绝口,但由于其自发性和无组织性,现在存在设计不得当,经营模式不健全等弊端.研究以河北省涞源县白石山景区为例,分析其存在的问题,旨在为旅游型住居模式的设计与经营提供更为合理的方法与策略.     

Reducing crude protein variability and maximizing savings when formulating corn-soybean meal-based feeds

Crude protein in corn and soybean meal have been documented to vary, and such inherent variability can result in under- or over-feeding of CP when feeds are formulated, leading to reduced bird growth, added input costs, and increased environmental pollution. The purpose of this study was to compare 2 grain-handling techniques and 2 feed formulation methods (linear vs. stochastic programming) to reduce CP variability in finished feeds and determine resulting costs or savings. The 2 grain-handling techniques were placing all the random batches of each delivered ingredient in to (1) a single bin (1-bin method) or (2) segregating above- and below-average samples into 2 bins (2-bin method). A fast way of estimating the composition of the ingredients is now available (near-infrared reflectance spectroscopy). Microsoft Excel workbooks were constructed to solve broiler starter feed formulation problems. Formulating feeds by linear and stochastic models based on the 2-bin method reduced CP variability by at least 50% compared with the 1-bin method. Formula cost was reduced by ˜20 cents per ton (averages of August 2012 United States ingredient prices) when the 2-bin method was used with the linear model. Formulating feed with a margin of safety increased formula cost by $3.40 per ton. Stochastic feed formulation increased formula cost to meet the specified CP level in feed at any probability of success, and formula cost was reduced substantially with the 2-bin method (up to $6.47 per ton). The magnitude of savings and reduced feed variability suggested that, regardless of the costs associated with building extra bins, the 2-bin method can be economically efficient in the long run. Therefore, it could be possible to split the batches of feed ingredients at a feed mill into above- or below-average bins before feed formulation to reduce CP variability and to maximize savings.