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71.
72.
Sarah Cox M.A. Mayo A. Teifion Jones 《European journal of plant pathology / European Foundation for Plant Pathology》2000,106(4):353-364
Analysis was made of dsRNA in 37 cultivars and species of Ribes, that were healthy, naturally affected with the virus-like diseases, blackcurrant yellows, blackcurrant infectious variegation, gooseberry veinbanding or blackcurrant reversion, or graft-inoculated with scions from such diseased plants. Various dsRNA species, differing in size (from ca. 2 to 11kbp), number and staining intensity in gels, were detected in some or all assays of all plants, including those held as virus-tested stock. In different plant tissues from individual plants, the dsRNA species were usually similar in size and number but, in some sources, the dsRNA profile from flowers and/or bark differed greatly from the profiles of dsRNA obtained from leaves. No dsRNA species were associated consistently with any of these diseases. A 499kbp cDNA probe was obtained that in Northern blot analysis was specific to a ca. 5kbp dsRNA species present in the blackcurrant cv. Baldwin. It also detected a similarly sized dsRNA species in plants of many other blackcurrant cultivars, but it did not react with a similarly sized dsRNA species in redcurrant and gooseberry tissues. The 156 amino acid sequence encoded by the cDNA was very similar to sequences in the RNA-directed RNA polymerases of virus species in the family Totiviridae, especially Saccharomyces cerevisiae viruses L-1 and L-A. The significance of these findings and the possible origin of these dsRNA species are discussed. 相似文献
73.
生物技术在畜牧业上的应用研究 总被引:1,自引:0,他引:1
从生物技术与畜禽品种、动物遗传育种,动物饲料资源开发,动物疫病的预防与诊断几个方面,论述了其在畜牧业中的应用,以期用生物技术来推动我国畜牧业的更快发展。 相似文献
75.
76.
Julie M. Steed Andreas Baierl Bruce D. L. Fitt 《European journal of plant pathology / European Foundation for Plant Pathology》2007,118(4):359-373
In winter oilseed rape experiments at Rothamsted in 2000/01 to 2002/03 growing seasons, the severity of phoma stem canker
epidemics in summer depended on the timing of phoma leaf spot epidemics in the previous autumn, and hence on the timing of
Leptosphaeria maculans ascospore release. The first major release of L. maculans ascospores was earlier in 2000 (26 September) and 2001 (18 September) than in 2002 (21 October). Consequently, the autumn
phoma leaf spot epidemic was also earlier in 2000 and 2001 than in 2002. The resulting stem canker epidemics were severe by
harvest (July) in 2001 and 2002 but not in 2003. No correlation was found between the severity or duration of phoma leaf spotting
(lesion days or lesion °C-days) and the subsequent severity of phoma stem canker epidemics. Rates of leaf production and loss
were similar in the three growing seasons. Out of ca. 25 leaves produced on plants during each season, leaf numbers 10–14 generally remained on plants for the longest. Treatment
with flusilazole + carbendazim in autumn decreased the severity of phoma leaf spotting for several weeks after treatment,
decreased the severity of stem canker the following summer and increased yield significantly in 2001 and 2002 but not in 2003.
The most effective timings for flusilazole + carbendazim application were when leaves 7–11 were present on most plants and
at least 10% of plants were affected by phoma leaf spot. Two half-dose applications of fungicide reduced phoma stem canker
and increased yield more than a single full dose application when phoma leaf spot epidemics were early (<800 °C-days after
sowing). 相似文献
77.
2003年秋至2005年春2个油菜生长季分别到湖北和安徽2省12县市油菜产区进行病毒病发病情况调查,除局部地区和地块发病率达到20%~40%,多数大面积油菜发病率在0.1%以下。应用间接酶联免疫吸附法检测258份病害样品,TuMV占样品总数的90.7%,CMV占样品总数的8.9%,ORMV占样品总数的0.8%。 相似文献
78.
79.
喷克(mancozeb)80%可湿性粉剂600倍,800倍,1000倍室内对苹果轮纹(Physalosporapiricola)抑菌效果均达100%,600倍,00倍对苹果斑点落叶病菌(Alternariamali)炭疽病菌(Glomerellacingulata)亦具显著的抑制效果,人工接菌条件下,800倍液防治苹果斑点落叶病的实际保护期主10-12d,防治苹果轮纹病的有效保护期为8-10d。田 相似文献
80.
R. J. Hillocks 《Integrated Pest Management Reviews》1998,3(3):155-167
Weed control is one of the most important crop protection activities undertaken in both intensive and low-input farming systems. However, even under intensive systems, crop protection which is less dependent on pesticides may require that weeds be managed to obtain a balance between crop and non-crop vegetation to encourage an increase in natural enemies of crop pests. In the low-input farming systems which sustain much of the rural population of Africa, weed control is usually done by hand and clean weeding is often beyond the labour resources of the farming family. The vegetational diversity of peasant agriculture in Africa to which weeds make their contribution, helps to decrease the risk of disease and pest epidemics. In addition to the pest control benefits of a diverse agroecosystem, weeds contribute to the resource base of the rural community, providing a source of secondary foods, medicines and insecticides. Weed control within an integrated crop protection system appropriate to the needs of the resource-poor farmer, requires that weeds are managed in such a way that their biodiversity is maintained and the more useful species retained within the field or field margin. Those weeds with high food potential or which have pesticidal or medicinal properties might be deliberately encouraged within the crop or field margins. Certain weed species may harbour important pests or diseases of local crops and therefore should be selectively removed. The paper reviews and discusses the literature on the beneficial and deleterious effects of weeds and argues for a weed management strategy which balances the effects of weed competition on crop production with the ethnobotanical and pest control attributes of individual weed species and weed communities. 相似文献