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| 转基因抗病毒病四倍体西瓜的培育 | |
| 引用本文： | 黄学森,牛胜鸟,王锡民,于嘉林,赵福兴,王生有,翟光明,蔡晓雨,师范生,曹永刚,许艳,郑芳,王小红,金霞.转基因抗病毒病四倍体西瓜的培育[J].中国瓜菜,2007(6):1-4. |
| 作者姓名： | 黄学森 牛胜鸟 王锡民 于嘉林 赵福兴 王生有 翟光明 蔡晓雨 师范生 曹永刚 许艳 郑芳 王小红 金霞 |
| 作者单位： | 1. 山西金鼎生物种业股份有限公司,山西运城,044000 2. 中国农业大学生物技术,国家实验室,北京,100094 3. 新加坡国立大学,117543 |
| 摘 要： | 利用西瓜花叶病毒2号(WMV-2)外壳蛋白(CP)基因、小西葫芦黄花叶病毒(ZYMV)复制酶(NIb)基因和黄瓜花叶病毒(CMV)复制酶基因构建三价基因的植物表达载体,通过农杆菌介导转化四倍体西瓜植株,经分子检测证明目的基因成功地导入西瓜植株,并在后代中稳定遗传。经温室及大田接种鉴定,T3代转基因西瓜抗病毒性达中抗水平,为抗病毒无籽西瓜新品种的培育提供了可能性。 |
| 关 键 词： | 四倍体西瓜 病毒病 转基因 抗病性 |
| 收稿时间： | 2007-09-24 |
| 修稿时间： | 2007年9月24日 |
Breeding of transgenic tetraploid watermelon resistant to viruses |
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| HUANG Xue-Sen,NIU Sheng-Niao,WONG Sek-Man,YU Jia-Lin,ZHAO Fu-xin,WANG Sen-you,ZHAI Guang-Ming,CAI Xiao-Yu,SHI Fan-Sheng,CAO Yong-Gang,XU Yan,ZHENG Fang,WANG Xiao-Hong,JIN Xia.Breeding of transgenic tetraploid watermelon resistant to viruses[J].China Cucurbits And Vegetables,2007(6):1-4. | |
| Authors: | HUANG Xue-Sen NIU Sheng-Niao WONG Sek-Man YU Jia-Lin ZHAO Fu-xin WANG Sen-you ZHAI Guang-Ming CAI Xiao-Yu SHI Fan-Sheng CAO Yong-Gang XU Yan ZHENG Fang WANG Xiao-Hong JIN Xia |
| Institution: | 1. Shanxi Jinding Biology Seed Co. Ltd, Yuncheng, Shanxi, 044000, China ; 2. China Agricultural University, Beijing, 100094, China ; 3. Notional University of Singapore ,Singapore, 117543 |
| Abstract: | By using Agrobacterium tumefaciens tetraploid watermelon was transformed with the construct consisting of the coat protein gene of WMV-2 and replicase genes of ZYMV and CMV. Presence of alien genes in the transgenic watermelon in the progenies of T1,T2 and T3 was confirmed by PCR-Southern blot. Greenhouse testing of the T3 generation transgenic lines showed intermediate resistance to viruses. It is,therefore,possible to breed the new variety of seedless watermelon with resistance to viruses. |
| Keywords: | Tetraploid watermelon Virus diseases Genetic transformation Resistance to virus |
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