鸭病毒性肝炎鸭胚灭活疫苗的研究

以鸭病毒性肝炎病毒Ⅰ型ATCC毒株接种健康鸭胚,收集致死的全胚组织作制苗材料,用福尔马林灭活,以麸氨酸钠终止其作用。先后试制疫苗11批,在试验室免疫雏鸭71只,经强毒攻击后,总的保护率为90.4％。本疫苗免疫雏鸭3天后可产生较坚强的免疫力,在室温至少可保存11天,在4℃可保存254天。经在江苏、安徽、广东等省推广应用200000头剂,均安全有效,颇受用户欢迎,对控制鸭病毒性肝炎的流行起了重要作用。     

USE OF 123IODINE METAIODOBENZYLGUANIDINE SCINTIGRAPHY FOR THE DIAGNOSIS OF A PHEOCHROMOCYTOMA IN A DOG

A 13-year-old neutered female Yorkshire terrier presented with a history of progressive episodic weakness and disorientation of 4 months duration. Physical and neurologic examinations were normal at presentation. Abdominal ultrasound revealed a mass involving the right adrenal gland. Standard planar scintigraphy was performed at 4, 18, and 24 hours after intravenous injection 185 MBq (5mCi) of ¹²³I-labeled metaiodobenzylguanidine (¹²³I-MIBG). An area of focal intense uptake was identified in the area of the right adrenal gland. A pheochromocytoma was confirmed histologically after surgical excision.     

PCR技术检测猪伪狂犬病毒及其潜伏感染部位的研究

本研究合成了伪狂犬毒糖蛋白ｇｐ５０基因引笺，该引物能够扩增糖蛋白ｇｐ５０基因中４３４－６５１之间的２１７ｂｐＤＮＡ片段，该片段含 ＳａｌＩ酶切位点，应用引物对几种不同的伪狂犬病毒株多聚酶链式反应扩增结果全为阳性。     

动物病毒性传染病的基本特征

动物病毒病几乎占传染病中的80％，经长期研究和查阅大量文献资料后，发现病毒性传染病具有许多独特的特征，了解掌握这些特征对病毒病的诊断，预防，治疗具有重要的指导作用。     

IOHEXOL AND IOPAMIDOL MYELOGRAPHY IN THE DOG: A CLINICAL TRIAL COMPARING ADVERSE EFFECTS AND MYELOGRAPHIC QUALITY

In a blind clinical trial, adverse effects after iohexol and iopamidol myelography were evaluated in 151 dogs. Eighty-one dogs were given iohexol (240 mgI/ml) and 70 dogs were given iopamidol (200 mgI/ml) by pre-determined assignment. Each dog was evaluated postmyelographically for seizures, hyperthermia, prolonged recovery from anesthesia and intensification of pre-existing neural signs. Myelographic quality was evaluated with a subjective scoring method. In comparing iohexol and iopamidol groups, there was not a statistically significant difference in the incidence of adverse effects or in myelographic quality. Iopamidol and iohexol appeared to be equally efficacious for routine canine myelography.     

牛流行热病毒灭活疫苗免疫后牛外周血淋巴细胞亚类分布的研究

对牛流行热病毒灭活疫苗免疫并攻每后，牛外周血淋巴细胞亚类的变化进行了研究。结果表明，灭活苗免疫后，牛的CD4^ 显著升高，可能与参与辅助B淋巴细胞合成抗体有关，攻毒后，γδT细胞均显著上升，免疫组在攻毒后3周仍保持在相当的高水平，IL－2Rα阳性细胞在攻毒后高热期也有显著升高，而CD8^ 细胞没有明显变化。     

鸡白血病病毒抗原ELISA试剂盒的研制和应用

以双抗体夹心酶联免疫吸附试验（ＤＡＳ－ＥＬＩＳＡ）为基本原理，成功地研制出鸡白血病病毒抗原ＥＬＩＳＡ试剂盒，该试剂盒与国外同类试剂盒相比，具备相同的特异性和敏感性，对ＲＡＶ－１纯化样品的最小检出量可达０．７８ｕｇ／ｍｌ，且敏感性高于直接补体结合试验。经初步应用发现，我国商品种鸡群ＡＬＶ阳性率为０．７－１４％，在部分ＳＰＦ鸡群中未检出阳性鸡。     

Recent studies on the enterotropic strain of avian infectious bronchitis virus

Avian infectious bronchitis (AIB) is an economically important disease of chickens. Recent studies have revealed enterotropism by at least one strain of AIB virus with pathological lesions in parts of the gut. This review highlights the findings of the studies so far made on this enterotropic strain of AIB virus.     

马立克氏病毒单克隆抗体杂交瘤细胞株的建立与鉴定

以马立克氏病毒814株(MDV-814)作为免疫用抗原,建立了2个MDV特异性单克隆抗体(McAb)杂交瘤细胞株—4A6、3D7。鉴定结果表明;两株细胞所分泌的McAb均为IgM类免疫球蛋白,具有MDVⅠ型病毒特异性,无中和反应特性和沉淀反应特性。利用两种McAb对国内标准强毒MDV-京1株进行鉴定,肯定其为MDV-Ⅰ型毒株。     

Evaluation of Transmittance and Reflectance Pulse Oximetry in a Canine Model of Hypotension and Desaturation

Ten, anesthetized dogs were instrumented with three pulse oximeter probes; two lingual transmittance probes and one rectal reflective probe. Arterial oxygen desaturation was produced by decreasing the inspired oxygen concentration. Hypotension was produced with an infusion of nitroprusside. Simultaneous pulse oximeter readings (SpO₂) were compared to co-oximeter measured arterial saturation (SaO₂) collected over a range of SaO₂ (50–100%) and mean arterial pressures (40–100mmHg). Each of the monitors and means of evaluating SpO₂ studied provided accurate SpO₂ measurements over a range of mean arterial pressure from 40–100mmHg. All of the monitors tested tended to overestimate the SaO₂ when the arterial saturation was less than 70%.