Inhibitory Effects of AURKB Gene on Apoptosis and Cancer Cell Growth in HCT 116 Cells

[Objectives]To explore the inhibitory effect of AURKB gene in apoptosis and cancer cell growth in HCT 116 cells.[Methods]The in vitro cytology studies were carr...     

Effect of beclin-1 gene silencing on TuAKe-injured gastric cancer SGC-7901 cells

AIM: To observe the effect of beclin-1 silencing by the technique of RNA interference on the injury of human gastric cancer SGC-7901 cell by Sheliugu extract (the extract from tuber of Amorphophallus konjac, TuAKe). METHODS: To knock down the expression of beclin-1 gene, SGC-7901 cells were transfected with lentiviral vector carrying beclin-1-shRNA. The beclin-1 gene knock-down and non-knock-down SGC-7901 cells were treated with TuAKe. The cell viability was analyzed by CKK-8 assay. The percentages of apoptotic cells were detected by flow cytometry. The expression of beclin-1 and LC3 was detected by Western blot. RESULTS: The beclin-1 gene silencing decreased the protein expression of beclin-1 and increased the protein expression of LC3 in the SGC-7901 cells, leading to the decrease in cell viability and the increase in apoptotic rate (P<0.05). TuAKe increased the protein expression of beclin-1 and LC3 in the SGC-7901 cells, and decreased the protein expression of LC3 in the SGC-7901 cells with beclin-1 gene silencing, thus inhibiting the cell viability and increasing the apoptotic rate (P<0.05). CONCLUSION: Beclin-1 gene silencing inhibits the activation of beclin-1-related signaling pathway in gastric cancer SGC-7901 cells, and aggravates the injury of cell viability induced by TuAKe.     

miR-204 inhibits proliferation of Hodgkin lymphoma cells by targeting Sirt1/p53 signaling pathway

AIM: To investigate the effect of microRNA-204 (miR-204) on the proliferation of Hodgkin lymphoma cells and the underlying mechanism. METHODS: The expression of miR-204 and Sirt1 mRNA in Hodgkin lymphoma tissues was detected by RT-qPCR. After transfection with miR-204 mimic, Sirt1 siRNA and miR-204 mimic+pcDNA3.1-Sirt1 into the L428 cells, the cell viability and BrdU incorporation were measured by CCK-8 assay and BrdU assay, respectively. The protein levels of Sirt1 and acetylated p53 (ac-p53) were determined by Western blot.The targeting relationship between miR-204 and Sirt1 was verified by double luciferase reporter assay. RESULTS: The low expression of miR-204 and the high mRNA expression of Sirt1 were found in the Hodgkin lymphoma tissues. Compared with control group, the cell viability, BrdU incorporation and the protein levels of Sirt1 and ac-p53 were significantly decreased after L428 cells were transfected with miR-204 mimic or Sirt1 siRNA (P<0.05). Compared with miR-204 mimic alone group, the cell viability, BrdU incorporation and the protein levels of Sirt1 and ac-p53 were increased after L428 cells were co-transfected with miR-204 mimic and pcDNA3.1-Sirt1 (P<0.05). The results of double luciferase reporter assay confiermed that Sirt1 was the target gene of miR-204. CONCLUSION: The inhibitory effect of miR-204 on the proliferation of L428 cells may be achieved by inhibiting the expression of Sirt1 and promoting the up-regulation of ac-p53.     

Effect of proline-spirooxindole on viability and apoptosis of non-small-cell lung cancer A549 cells

AIM:To investigate the effect of proline-spirooxindole on the viability and apoptosis of human non-small-cell lung cancer A549 cells. METHODS:The effect of proline-spirooxindole on the viability of A549 cells was determined by CCK-8 assay. The apoptosis was analyzed by flow cytometry. The effects of proline-spirooxindole on the expression of PARP and p53 and the phosphorylation of mTOR were determined by Western blot. RESULTS:After A549 cells were treated with proline-spirooxindole (25, 50 and 100 mg/L), the cell viability was decreased (P<0.01) compared with DMSO control group. The apoptotic rate was increased compared with DMSO control group (P<0.01). The protein expression of p53 was up-regulated, the increased apoptotic protein cleaved PARP was observed, and the phosphorylation of mTOR was inhibited (P<0.01). CONCLUSION:Proline-spirooxindole inhibits the viability of A549 cells and induces apoptosis, which may be related to the phosphorylation of mTOR.     

红蓝LED光照强度对茶树生长及生物化学成分的影响

以‘黔湄601’一年生茶树苗为试材,光量比为1∶3的LED红蓝光作为光源,探讨不同光照强度对茶树幼苗生长特性的影响、光合色素及生物化学成分的变化趋势,探索茶树苗生长发育对光强的响应机制。采用LED精准调节光质和光强,设置50μmol·m-2·s-1、100μmol·m-2·s-1、150μmol·m-2·s-1、200μmol·m-2·s-14个处理。结果表明,不同光强处理15 d后,50μmol·m-2·s-1光照强度有利于茶树叶色素含量的增加及Ca/Cb值的降低;100μmol·m-2·s-1光照强度有利于茶树叶茶多酚含量的降低及氨基酸含量的增加,酚氨比值最低;150μmol·m-2·s-1光照强度对茶树叶重增加有促进作用;200μmol·m-2·s-1光照强度有利于茶树叶茶多酚含量的增加和氨基酸、光合色素含量的减少;酚氨比值最高,光合色素含量最低。红蓝LED光照强度过低、过高都不利于茶叶品质形成,综合考虑,100μmol·m-2·s-1光照强度最有利于茶叶功能成分的积累,是茶叶LED光源设施栽培的理想光照强度。本研究结果对于设施茶树种植具有重要的参考意义。     

围产期饲喂过瘤胃胆碱对奶牛生产性能及血液生化指标的影响

本试验旨在研究围产期奶牛饲喂过瘤胃胆碱(RPC)对泌乳性能、血液生化指标及繁殖性能的影响。试验采用单因素试验设计,选用60头年龄、胎次、预产期、上一胎次产奶量相近的健康围产期奶牛,随机分为3组,每组20头。Ⅰ组饲喂基础日粮,Ⅱ组和Ⅲ组在基础日粮上分别添加25 g/d和50 g/d RPC,饲喂期从产前3周至产后3周,共42 d。结果表明:Ⅱ组产奶量在产后1~8周显著高于Ⅰ组和Ⅲ组(P0.05);在产后第2周,Ⅲ组的乳脂率、乳蛋白率显著高于Ⅰ组(P0.05);在整个试验期,Ⅱ组的谷丙转氨酶含量显著低于Ⅰ组(P0.05),Ⅲ组的天门冬氨酸转氨酶显著含量低于Ⅰ组(P0.05);产后第1周和第2周,Ⅱ组的β-羟丁酸、非酯化脂肪酸含量显著低于Ⅰ组(P0.05);Ⅱ组的甘油三酯含量显著高于Ⅰ组和Ⅲ组(P0.05),Ⅲ组的总胆固醇含量显著高于Ⅰ组(P0.05);日粮中添加RPC可以显著提高情期受胎率(P0.05),显著缩减奶牛空怀天数(P0.05),也对产后初次配种时间的缩短和配种次数的降低有影响。日粮中添加RPC可提高围产期奶牛生产性能,对血液生化指标和繁殖性能产生有利影响,本试验条件下RPC最佳添加剂量为每头牛25 g/d。     

古柏赤枯病病原菌的研究

经Ｋｏｃｈ＇ｓ法则检验证明，ＡｌｔｅｒｎａｒｉａｔｅｎｕｉｓＮｅｅｓ（细链格孢霉）是北京地区古柏赤枯病的病原菌。它引起古柏针叶及嫩梢的大量死亡。２—３年生桧柏及侧柏苗经人工接种证明，该病菌有较强的致病力，它还侵染某些种类的杨树。该病潜育期为５—６天。对病菌生物学特性也作了研究。病原菌生长和孢子萌发最适温度为２５℃Ｆ相对湿度在９０％以上；最适ｐＨ值为６；葡萄糖、淀粉、蛋白胨、谷氨酸为该菌纯培养的优良营养源。     

不同稳定剂对胭脂萝卜红色素稳定性的影响

天然胭脂萝卜红色素属于花青素类化合物,性质不稳定。笔者以新鲜胭脂萝卜(Raphnussativuscv.)肉质根为原料,提取胭脂萝卜红色素,选择常用的5种稳定剂对其稳定性进行研究,从而确定出最佳的稳定剂及其用量。研究结果发现0.03%维生素C、0.03%谷氨酸、0.03%柠檬酸能明显地延缓其颜色变化,0.03%维生素C 0.03%柠檬酸的组合对该色素的稳定效果最佳;进一步研究确定维生素C和柠檬酸的最佳用量分别为0.04%和0.08%。     

Corneocyte Counts for Evaluation of Antiseborrheic Shampoos in Dogs

Abstract— An objective method for measuring corneocyte numbers before and after antiseborrheic shampoo therapy was assessed in dogs. Corneocytes were collected from six skin regions on the dorsal trunk of nine colony-raised beagles with clinically normal skin. Forty-eight collection sites were randomly assigned a treatment with one of seven medicated shampoos or water alone; six additional sites served as non-treated controls. Corneocytes were collected immediately before the first treatment, and 14 and 28 days after initiation of therapy with a 0.1% Tween 80 surfactant and a quantitative cup-scrub technique. Pretreatment corneocyte counts were not significantly different among the nine dogs nor among the randomly assigned treatment and control sites (3.55 · 10⁴ cells.cm^-2, SEM 0.17). Corneocyte counts increased in all treatment and control sites from days 0 to 14. Corneocyte counts were similar in the non-treated and water-treated sites from days 14 to 28. Corneocyte counts were not significantly different when the same treatments were compared on days 0, 14 and 28, or when different treatments were compared on the same day. The corneocyte collection technique used in this study proved to be a reliable method for assessing the rate of cell desquamation and cell surface cohesion in dogs.     

日粮色氨酸水平对仔猪生产性能和血液生化指标影响的研究

在赖氨酸/蛋氨酸+胱氨酸/苏氨酸/异亮氨酸比例为100:68:65:59的玉米—玉米蛋白粉基础日粮中,添加合成的 L-色氨酸构成5种色氨酸(TRP)水平的日粮(TRP 水平分别为0.125%、0.155%、0.180%、0.215%和0.24%)。将35头太湖母猪与长白公猪的杂交仔猪在35日龄断奶时,随机分为5组,分别饲喂上述为同 TRP 水平的日粮,饲养期长达30天。结果表明,日粮(TRP)水平显著影响仔猪的日均耗料量(ADFI)、日均增重(ADG)和饲料转化率(FCR)。TRP 水平为0.215%时,仔猪生长性能最好。TRP 水平为0.24%时仔猪的生长性能与0.215%时差异不显著(P>0.05)。TRP 水平在0.125%～0.215%范围内,仔猪 ADG、ADFI 与 TRP 水平呈正相关,回归方程分别为:ADG(g)=2.57 TRP(mg/kg)-80.21,(r=0.99,P<0.01);ADFI(g)=2.49 TRP(mg/kg)+220.31,(r=0.95,P>0.05);FCR 与日粮 TRP 水平呈显著负相关,回归方程为:FCR=2.877-5.668 TRP(%),(r=0.945,P<0.01).血清尿素氮(SUN)含量与日粮 TRP 水平呈负相关,回归方程为 SUN=19.47-34.35TRP(%),(r=0.95,P<0.05)。从仔猪生长性能和 SUN 两项指标确定 TRP 在理想氨基酸模式中的相应值分别为18和20。在 TRP 水平0.125%～0.215%范围内,血清碱性磷酸酶活性与 TRP 水平呈现正相关,在0.215%时最高;TRP 水平在0.24%时与0.215%时差异不显著(P>0.05)。日粮 TRP 水平对血清谷丙转氨酶(GPT)和谷草转氨酶(GOT)活性无显著影响(P>0.05)。日粮 TRP 水平对血清总蛋白含量无显著影响,而血清蛋白含量随 TRP 水平升高而增加,在0.215%时最高。血清蛋白质系数(A/G)在各 TRP 水平间有显著差异(P<0.05),日粮中 TRP 含量在0.125%时,A/G显著降低(P<0.05),A/G 在 TRP 水平为0.215%时最高,TRP 在0.125%～0.215%之间,A/G 呈现线性递增,回归方程为:A/G=9.34TRP(%)+0.956,r=0.98,P<0.05),而 TRP 水平为0.24%与0.215%的两组 A/G 无显著差异(P>0.05)。