西伯利亚百合脱毒技术研究

运用茎尖培养、热处理结合茎尖培养、病毒唑结合茎尖培养、热处理加病毒唑结合茎尖培养4种方法，脱除西伯利亚百合品种CMV、LSV和LMoV病毒，分析了4种处理后的成苗率和脱毒率。结果表明：以热处理加病毒唑结合茎尖培养法效果最为明显。     

优质抗寒、抗病酿造葡萄新品种‘北红’

 ‘北红’葡萄新品种由‘玫瑰香’与‘山葡萄’杂交育成。浆果在北京地区9月底成熟。果粒圆形，蓝黑色，果粒质量1.57g，果穗质量160.0 g，浆果可溶性固形物含量23.8 %～ 27.0 %，含酸量0.89～1.26 %。早果性及丰产性强。抗寒、抗病能力特强。酿成的酒深宝石红色，酒体平衡，酒质上等。     

盐城市病虫危害环境污染状况及对策

环境污染已成为当前蚕桑安全生产的最大危害,桑树病虫为害也呈趋重的态势。探讨了盐城市病虫危害、环境污染状况及其防制的主要做法和设想。     

Research Progress Towards the Liquid Chip Technology in Detection of Animal Infectious Diseases

Liquid chip technology is a novel biomolecular detection technology which integrates laser technology,flow cytometry,digital signal processing and traditional chemical technology.It is widely used in various immunological analysis and nucleic acid detection.Single and mutiplex analysis are supported,with protein and nucleic acid targets detected in a variety of detection methods in high throughput manner.It has advantages of high throughput,easy operation,wide range of application,good repeatability,high specificity,less sample needed,high sensitivity and stablility,and low cost.Therefore,they are gradually replacing the traditional detection and quantitative pathogen methods,such as Real-time fluorescent quantitative nucleic acid amplification detection system (qPCR),enzyme-linked immunosorbent assay (ELISA) and other detection methods.Animal infectious diseases seriously endanger the development of the breeding industry,futhermore,some zoonoses such as highly pathogenic avian influenza also pose a serious threat to human health.An efficient and sensitive diagnostic system will help to screen a large number of samples during the outbreak of infectious diseases and prevent the spread of infection.The development of liquid chip technology provides a new platform for high-throughput detection and disease prevention.In this review,the principle and advantages of liquid chip technology are briefly described.The research progress of liquid chip technology in the detection of animal infectious diseases,including pigs diseases,poultry diseases,rabbit diseases,dog diseases,rodent diseases and other animal infectious diseases are summarized.We believe that in the future,this technology will become an important analytical and testing tool in clinical diagnosis,basic research,new drug development,judicial identification,food health supervision,biological weapons prevention and other fields.The development of this technology will greatly promote the research and development of life science.     

Effects of Diseases on Reproductive Performance in Dairy Cattle Ⅰ: Current Situation and Its Mechanism

Over the past 20 years,with the continuous promotion of the genetic improvement plan of dairy cow population and the continuous improvement of feeding technology,the intensive level and milk yield of dairy cow population in China are continuously improving.However,the reproduction data over the years showed that the more milk yield,the more obviously decline in reproductive performance,and the more prominent of the reproductive problems.The decline of fertility caused by high yield has become the bottleneck of the development of dairy industry in China and even in the world.In dairy farming,there are many factors that cause the reduction of reproductive performance of high-yield dairy cows,including genetic factors,environmental factors and disease factors.Recently,the influence of disease factors on reproductive performance of dairy cows has become more and more prominent.According to the location of the disease,the diseases can be divided into genital diseases and non-genital diseases.This paper discussed the relevant research data on the effects of different diseases on the reproductive performance of dairy cows in recent years,and focusing on the analysis of the molecular mechanism of diseases affecting the reproductive performance of dairy cows through the nervous system,reproductive endocrine system and humoral immune system.The prospect and thinking for the future research of diseases and reproductive performance of high-yield dairy cows were put forward to provide reference and theoretical basis for improving the reproductive efficiency and economic benefits of high-yield dairy cows in China.     

Epidemiological studies of pig diseases: 1. Use of French protocols for risk factor assessment to predict the health status of Australian herds

Objective To determine in Australian pig herds the accuracy of French protocols for risk factor assessment.
Procedure Data on health indicators and risk factors were collected for three syndromes, 'pre-weaning diarrhoea', 'post-weaning diarrhoea' and 'respiratory problems', using the French protocols. The protocols were used on 118 occasions in 32 Western Australian pig herds during 3 years (1988 to 1991).
Results There was a wide variation in pre-weaning performance, for example growth rate was 107 to 273 g/day (< 200 g/day in 33% of herds). Respiratory lesions at weaning were associated with poor pre-weaning performance. Post-weaning (21 days after weaning) growth rate was 114 to 408 g/day (< 250 g/day in 54% of herds). In the grower herds, 91% of herds had pneumonia, and growth rate was 439 to 625 g/day (< 550 g/day in 54% of herds). Pleurisy as well as pneumonia was associated with reduced growth rate. The risk factor most closely associated with respiratory health status was air volume per pig.
Conclusion Risk factors were most accurate at predicting the health status in post-weaning problems. A weaning weight of at least 7.9 kg and weaning age of 30 days optimised weaner performance. Stocking densities and shed designs providing at least 3 m³ air volume and 0.6 m² floor space per pig throughout the growing phase should be considered for an improved respiratory health status. Australian pig sheds often do not provide a satisfactory environment for optimum health. The technique of risk factor assessment as an aid to the maintenance of health in pig herds is applicable in Australia, but further research is necessary to determine the most important Australian risk factors.     

大棚辣椒病虫害全程绿色防控研究

摘要：针对大棚辣椒多年连续种植，导致大棚辣椒病虫害发生逐年加重、辣椒大幅减产、品质下降等问题，基于大棚辣椒主要病虫害发生特点及规律，通过试验和示范、推广，形成了1套以生态调控技术、生物防治技术、害虫诱杀技术和科学用药技术等为主要内容的全程绿色防控模式，并在生产中不断推广应用，病虫害防治效果达95%以上，化学农药使用量减少50%以上，农药残留合格率100%，提高了辣椒产量和品质，保证了农产品质量安全，生态效益显著；绿色防控区产投比（6.781）明显高于常规防控区（4.681），667 m2净收益较常规防控区高出4 111.0元，经济效益较好。     

银杏丰产栽培技术

银杏为我国传统的名贵树种,其生长缓慢、结实晚,一般要20多a才能开花结实,并且产量低。阐述银杏的生物学特征、繁殖方式、栽培管理方法、修剪整形及病虫害防治等实用技术,旨在为扩大银杏园林生产、提高银杏的种植质量和产量提供有益的参考。     

云南林木真菌病害研究(Ⅰ)

 报道了云南林木真菌病害23种,其中:国内新记录4种,云南省内新记录11种,云南省内已知种新分布8种.前者有描述,其余以名录列后,拟供植保和植检工作参考.