Development of an Allergic Murine Model Induced by Recombinant Blo t 5 Allergen of Blomia tropicalis

To development an allergic murine model induced by the recombinant Blo t 5 (rBlo t 5) allergen, the Blo t 5 gene sequences were optimized, synthesized and inserted into the prokaryotic expression vector PQE80L. The Blo t 5 was expressed in E.coli, purified by NI-NTA agarose,and tested by Western blotting. The results showed that the rBlo t 5 could specially react with monoclonal antibodies against Blo t 5. Twelve BALB/c mice were randomly divided into control group (PBS group, n=6) and rBlo t 5-induced group (rBlo t 5 group, n=6). After sensitization by intraperitoneal injection (i.p) and challenged by intratracheal instillation of rBlo t 5, the total serum IgE levels were measured by ELISA; The mice in rBlo t 5 group were challenged with rBlo t 5 by intraperitoneal injection, and the body temperature was measured at regular intervals; The lung was fixed and stained with haematoxylin and eosin (HE).The result showed that compare with PBS group, the total serum IgE levels rised significantly in rBlo t 5 group (P<0.05). The body temperature dropped significantly and a severe allergic reaction (anaphylaxis) was occurred in mice of rBlo t 5 group (P<0.05).The mice in rBlo t 5 group showed inflammatory cell infiltration in airway by HE.These results showed that the allergic murine model was induced successfully by rBlo t 5 allergen.     

警犬真菌性皮肤病与过敏性皮炎的相关性分析

警犬是由警察机关使用，具有一定警务用途的犬种。警犬承担着刑侦破案、安保安检、追踪搜索、巡逻、灾后救援等特殊勤务，保证警犬的身体健康、安全具有重要意义。真菌性皮肤病是警犬常见的疾病之一，但该病常常愈后不佳，且与过敏性皮炎存在密切联系，因此对二者的相关性研究对临床治疗起到一定作用。本文总结了警犬真菌性皮肤病及过敏性皮炎相关文献报道，对于警犬真菌性皮肤病与过敏性皮炎的相关性进行了探讨分析，旨在提高警犬皮肤病防治效果，为预防警犬皮肤病提供一些依据。     

蜂蜜治疗花粉过敏

<正>《本草纲目》记载蜂蜜能解毒,柔而润泽,能润燥,缓而去急,能止心腹肌肉疱疹之疼痛。现代医学研究发现,蜂蜜能够预防过敏,这是因为蜂蜜中含有少量的蜂毒,蜂毒在临床上常用于治疗过敏性哮喘等过敏性疾病。另外蜂蜜中含有少量的花粉粒,花粉过敏的人可以经常喝点蜂蜜,使身体慢慢的产生对花粉过敏产生抵抗力。     

How Specific is Non-Hypersensitive Host and Nonhost Resistance of Barley to Rust and Mildew Fungi？

Full nonhost resistance can be defined as immunity, displayed by an entire plant species against all genotypes of a plant pathogen. Interesting biological questions are, whether the genes responsible for the nonhost status of a plant species have a general or a specific effectiveness to heterologous （＂nonhost＂） pathogens？ Is the nonhost resistance to pathogens of plant species that are related to the nonhost based on R-genes or on other types of genes？ We study this question in barley （Hordeum vulgare L.）, which is a near-nonhost to several rusts （Puccinia） of cereals and grasses. By crosses and selection we accumulated susceptibility and developed an experimental line, SusPtrit, with high susceptibility to at least nine different heterologous rust taxa such as the wheat and Agropyron leaf rusts （P. triticina and P. persistens, respectively）. At the microscopic level there is also some variation among barley accessions in the degree that the heterologous wheat powdery mildew （Blumeria graminis f.sp. tritici） is able to form haustoria in epidermal cells. So, also the genetics of the variation in level of nonhost resistance to heterologous mildew fungi can be studied in barley. Our data obtained on mapping populations involving three regular nonhost-immune accessions （Vada, Cebada Capa and Golden Promise） suggest that nonhost resistance is the joined effect of multiple, quantitative genes （QTLs） and very occasionally a major gene （R-gene？） is involved. Most QTLs have effect to only one or two heterologous rusts, but some have a wider spectrum. This was confirmed in a set of QTL-NILs. Those QTL-NILs are used to fine-map the effective genes. In some cases, a QTL region with effectiveness to several heterologous rusts might be a cluster of genes with a more narrow spectrum of effectiveness. Our evidence suggests that nonhost resistance in barley to rust and powdery mildew fungi of related Gramineae is not due to R-genes, but to pathogen species-specific quantitative resistance genes.     

大豆致敏蛋白脱敏方法研究进展

大豆是一种富含多种优质营养蛋白的主要生产原料,广泛应用于食品、饲料等领域,同时也含有能够引起过敏患者发生过敏反应的致敏蛋白,且含量较高,引起的过敏发病率在世界范围内逐年攀升,成为一个急需解决的生命健康问题。按照传统方法避免食用大豆产品并不可行,因此通过一定的脱敏方法降低大豆蛋白致敏性已成为研究热点。本文综述了大豆致敏蛋白的过敏机制、脱敏方法及低致敏性大豆制品的开发,并对其今后的研究方向做出了展望。     

春季养生:这五种水果要少吃

正水果虽好但要吃的正确,春季回暖,有五种水果大家注意不能多吃:1、菠萝问题:一部分人吃菠萝后会出现恶心、呕吐、腹痛腹泻、口唇发麻、皮肤发痒等过敏现象。原因:这是因为菠萝中含有生物甙和菠萝蛋白酶。为预防过敏反应,菠萝切好后一定要放入淡盐水中浸泡十多分钟,破坏菠萝蛋白酶后再吃。营养师:菠萝属于热带水果,含有丰富的膳食纤维和菠萝蛋白酶,对预防便秘和消化不良很有好     

宠物除跳蚤杀虫剂

正【产品名称】除跳蚤杀虫剂【适用范围】适用7日龄及以上的犬。【产品特点】1.高效快速杀灭跳蚤、虱子、螕虫等虫体虫卵。2.可阻断无脊椎动物神经调节功能,效果好,持续时间长。3.产品检测安全无毒,爱犬不慎舔用,无需担忧。4.适应多种环境,一瓶多用,可用于宠物经常去的地方,在室外可喷洒在活动范围周边,有效防止野外害虫侵害;宠物出门前后,在室内喷洒防止宠物将害虫带入家中。     

浅谈食品安全中的过敏问题

环境中能够刺激机体发生过敏反应的都可称为过敏原,食品过敏已成为食品安全的一个极为严重的问题。介绍食品过敏原的概念、种类、危害、检测分析方法,以及食品过敏原的管理与控制问题。     

猪皮炎肾病综合症预防与诊治

随着天气转暖,蚊虫增多,近一段时期一些猪场的猪只皮肤上出现急性红、粉红甚至紫斑性的皮疹,其与疥螨性皮炎、过敏性皮炎、渗出性皮炎和猪痘等有明显的区别,常导致病猪生长缓慢,饲料报酬降低,死亡率上升。这种病被称为猪皮炎肾病综合症,多发生于炎热的夏季,其他季节呈散发,常同其他疾病如附红细胞体病、链球菌病等混发。1主要症状猪皮炎肾炎综合症多发生在8~20周龄的生长育肥猪和保育后期猪。病猪精神萎靡,体重迅速下降,耳背、背部、下腹部和后躯臀部皮肤上出现     

豚鼠全身主动过敏试验剂量设计的探讨

目的,探讨全身主动过敏试验的剂量设计。方法,以丁酸氯维地平为供试品,设计全身主动过敏试验,并观察试验结果。结果,丁酸氯维地平全身主动过敏试验结果为阴性。结论,针对不同药物的全身主动过敏试验,应按照不同的方法进行实验设计,设计时应熟练掌握并满足《化学药物刺激性、过敏性和溶血性研究技术指导原则》针对全身主动过敏试验所提出的要求。