细鳞斜颌鲴规模化人工繁育技术

从试验研究和生产实践中总结出细鳞鲴规模化人工繁育技术,对具体技术进行了描述、量化说明和讨论。细鳞鲴规模化人工繁育是项综合技术,其关键技术是催产药物配伍及其用量、自然产卵受精脱粘材料的选用和鱼苗至夏花阶段的培育。最佳的催产药物是促黄体素释放激素LHRH—A2＋地欧酮（DOM）＋绒促性素（HCG）,雌鱼剂量（LHRH—A210μg＋DOM5mg＋HCG300 IU）／kg,雄鱼减半。自然产卵受精脱粘材料宜选用滑石粉、食盐液（滑石粉200g、食盐25g加水5kg搅匀）。鱼苗至夏花阶段的关键是要培育适口的天然饵料和掌握鱼苗下塘时机。     

A new image dataset for the evaluation of automatic fingerlings counting

Fingerling counting in fish farming is still a problem to be solved, although there are some technological solutions used in experimental cases and controlled environments. In this paper, a fingerlings counter is evaluated using a new image database with 448 videos of the Pintado Real® fingerlings species. A total of 21,402 combinations of 6 parameters of the fingerlings counter were tested. In the training phase, 314 videos were randomly selected, which represent 70% of the database. The remaining 30%, corresponding to 134 videos, were used for testing. Focusing on the parameters that best recognize and track the fingerlings of Pintado Real®, it was obtained Pearson Coefficient of 0.9803 and a quadratic average error of 2.65 when comparing the manual and automatic counting. The results obtained six parameters sets that achieved these metrics, reaching higher performance on the fingerlings counter from a new image database. The image database used in our research is available for researchers.     

斑鳠鱼种耗氧率和窒息点的测定

在水温 2 4 .4～ 2 6.0℃时对斑鱼种 (尾重 1.12～ 4 .61g )进行了耗氧率与窒息点测定。结果表明 :耗氧率与窒息点均随鱼体体重的增加而降低。平均尾重 4 .61g的斑耗氧率为0 .1580 mg/g.h ,窒息点为 0 .12 92 mg/L ;平均尾重 1.12 g的斑耗氧率为 0 .2 636mg/g.h ,窒息点为 0 .30 4 0 mg/L。白昼和夜晚耗氧率的差异不大     

史氏鲟与杂交鲟幼鱼生长对比试验

在流水条件下,对人工饲料驯化的史氏鲟和杂交鲟幼鱼的生长进行了比较试验,结果表明：经６０ｄ饲养,杂交鲟幼鱼平均体重由７５ｇ增至１２００ｇ,最大个体重１８００ｇ,体长４２０ｃｍ;史氏鲟幼鱼平均体重由７３ｇ增至６８２ｇ,最大个体重１２００ｇ,体长３１０ｃｍ;本文还就体长与体重关系作了初步分析,并测得杂交鲟幼鱼生长方程为：Ｌ杂＝４４１７Ｇ０４４８２（ｒ＝０９６０４）,史氏鲟幼鱼生长方程为：Ｌ史＝５９４０２Ｇ０３７５７（ｒ＝０９８４７）。     

花鲈鱼种的网围培育试验

从河口捕获的花鲈苗，通过水泥池驯养和分级培育，至体长达４～５ｃｍ后采取池塘网围分级培育。在２６～４０天的网围培育期内，经２～３次过筛分级，鱼种平均体长达７～１２ｃｍ，成活率在８０％以上，明显优于传统的鱼种培育法。     

湖白鲑幼鱼对常用水产药物敏感性的试验

在水温为 1 6～ 1 9℃的条件下 ,进行了敌百虫 ( 90 %晶体 )、NaCl、ClO2 、甲醛、CuSO4和FeSO4合剂 ( 5∶2 )对湖白鲑幼鱼 ( 2 2～ 74mm)的耐受性试验 ,试验结果表明 :湖白鲑幼鱼对 5种水产常用药物敏感性的大小顺序为 :敌百虫 ( 90 %晶体 ) >CuSO4和FeSO4( 5∶2 )合剂 >甲醛 >ClO2 >NaCl。NaCl、ClO2 、甲醛、CuSO4和FeSO4合剂 ( 5∶2 )可以作为湖白鲑的鱼病防治药物使用 ,敌百虫 ( 90 %晶体 )不能作为湖白鲑的鱼病防治药物使用。     

Effect of different dietary taurine levels on the conjugated bile acid composition and growth performance of juvenile and fingerling Japanese flounder Paralichthys olivaceus

This study investigated the effects of different taurine levels on the conjugated bile acid composition and growth performance of juvenile and fingerling Japanese flounder. Five level diets of dietary taurine were prepared by the supplementation of taurine (0, 0.1, 0.2, 0.5 and 1.5%) to a basal composition. Fish meal washed with 70% ethanol to remove taurine was used as the sole protein source. Two feeding experiments were carried out at 20 °C by using different sized fish (BW: 0.7 g and 9.6 g). Both size groups of fish were fed the experimental diets for 6 weeks. At the end of experiments, fish were weighed and stored at − 80 °C for analysis of free amino acids and conjugated bile acids composition. The body weight and feed efficiency of Japanese flounder were improved by taurine supplementation in the experimental diets. The taurine contents of the whole body and tissues increased with the increase of the taurine supplementation. The conjugated bile acids in gall bladder were composed with taurocholic acid (Expt.I: 33.0-146.8 mg/ml; Expt.II: 64.8-145.3 mg/ml) and taurochenodeoxycholic acid (Expt.I: 0.8-5.5 mg/ml; Expt.II: 3.5-4.9 mg/ml). These bile acids increased with the increase of the dietary taurine level. Taurocholic acid accounted for more than 95% of the total conjugated bile acids. No other conjugated bile acids except the taurine conjugated bile acids were detected in the gall bladder of Japanese flounder. This means that taurine is the sole amino acid to conjugate bile acid in Japanese flounder. Dietary taurine intake affects the conjugated bile acid composition in juvenile and fingerling Japanese flounder.     

四种抗菌促长药物在团头鲂饲料中适宜添加量的初步研究

用正交法设计了九种不同配方的团头鲂鱼种饲料。饲养结果表明,团头鲂鱼种配合饲料中磺胺二甲嘧啶的适宜添加量为50×10-6,黄霉素为2×10-6,碘化酪蛋白为20×10-6,痢特灵为100×10-6。     

异育银鲫高密度育种技术研究

为解决水库投放异育银鲫鱼种的需要,利用自繁的异有银鲫夏花在10个共4．27hm2池塘中进行了高密度育种技术研究。经60d饲养,在467250－520710尾／hm2的高密度下,成活率80％,饵料系数22,平均单产2445kg／hm2,最高单产3800kg／hm2,平均个体重6．3g,平均体长5．7cm,最大个体重18．5g,最大体长8．4cm。对高密度饲养条件下异育银鲫体长和体重关系作了分析,关系式为：W＝0．011L3．62。