喹乙醇对鲤鱼的急性毒性试验

以一龄越冬的德国镜鲤为试验对象，测定一次内服和腹注喹乙醇96h的死亡率，探讨喹乙醇对鲤鱼的毒性作用。试验结果表明，2－10g/kg体重的等比浓度梯度喹乙醇内服剂量不引起任何毒性反应，腹注喹乙醇LD50为1022.6mg/kg。结果提示，喹乙醇对鲤鱼的毒性极低。     

外源基因导入鲤（Cyp rinus carpio）受精卵最佳时期的研究

本研究以鲤鱼受精卵为材料，通过显微注射技术，在受精卵的不同发育时期把鲤鱼ＭＴ启动子基因和大麻哈鱼ＧＨ基因导入受精卵中，按孵出的鱼苗数分别计算成活率，再通过斑点杂交和Ｓｏｕｔｈｅｒｎ Ｂｌｏｔ杂交检测整合情况，计算整合率，孵化率最高的为单细胞中期（９０．８３％），整合率最高的为单细胞后期和囊胚期（４０％）。综合研究结果外源基因导入鲤鱼受精卵的最佳时期应选在单细胞后期。     

红鲤4群体间红细胞免疫功能及其差异

应用红细胞C3b受体花环试验和红细胞天然免疫粘附肿瘤细胞花环试验方法，证实兴国红鲤(Cyprinuscarpio.var.singguonensis)、玻璃红鲤(C.carpio.var.wananensis)、荷包红鲤(C.carpio.var.wananensis)及瓯江彩鲤(C.carpio.var.color)的红细胞表面都存在C3b补体受体，均可形成花环；红鲤的红细胞免疫粘附能力存在着种群间差异，上述2种花环试验的花环率大小的顺序均是：兴国红鲤＞荷包红鲤＞瓯江彩鲤＞玻璃红鲤，差异都极显著(P＜0．01)；4群体红鲤的红细胞均具有吞噬作用。结果证实，鱼类红细胞免疫功能在机体防御病原的过程中占有重要地位。     

溴化钠对鲤生长、甲状腺激素的影响及其在体内的蓄积

水体中添加不同浓度的溴化钠(NaBr)对鲤进行试验,分别在15、30和45d进行生长速度测定和组织蓄积研究,并在45d对甲状腺激素进行测定。研究发现,NaBr对鲤生长有促进作用,其中浓度为15mg·L-1的NaBr试验组促进作用最显著(P<0.05);NaBr影响甲状腺激素水平,浓度为15mg·L-1的NaBr试验组含量最高(P<0.01);鲤各组织中肝和皮的溴蓄积量较高,而肾在各浓度试验组均保持较高水平,浓度为25mg·L-1NaBr时肌肉中溴蓄积量为72.40mg·L-1;添加NaCl对鲤组织的溴蓄积量影响显著(P<0.01)。水体中NaBr含量低于25mg·L-1时,对淡水水生生物是安全的,所养殖生物中溴蓄积量符合食品卫生标准。     

Survival rate and growth performance of Cyprinus carpio L. in intensively cultivated rice fields

In the freshwater area of Vietnam's Mekong Delta, Barbodes gonionotus (Bleeker), Cyprinus carpio L. and Oreochromis niloticus (L.) are often reared together in rice fields. The survival rate and specific growth rate (SGR) of C. carpio showed a large variance. To understand the underlying reasons for this variation, we used multiple regressions for the variables SGR and survival rate, using the results of eight experiments in such polycultures at the Co Do rice–fish experimental station. The SGR of C. carpio declined with increasing rice density. Interspecific competition with O. niloticus also had a negative effect on C. carpio SGR. When fertilizer or manure is added to the trench of the rice–fish field or when extra food can be given to the fish, O. niloticus seems more appropriate than C. carpio. The survival rate of C. carpio depended on season, C. carpio stocking weight and density and the biomass of wild fish species. To obtain higher survival rates, one should stock fewer fish but of a larger size, after thorough eradication of wild fish species.     

饲料中添加鸡肠粉对鲤抗氧化及部分免疫酶活力的影响

为研究饲料中添加鸡肠粉对鲤(Cyprinus carpio)抗氧化能力和部分免疫酶活力的影响,配制了4组等氮等脂饲料和1组鸡肠粉含量为100％ 的饲料,标记为D1、D2、D3、D4、D5.鸡肠粉添加量分别为0％(D1)、5％(D2)、10％(D3)、15％(D4)和100％(D5).选取初始体重约为57 g的鲤150尾...     

复方磺胺甲噁唑在松浦镜鲤体内的药动学及残留消除规律

采用高效液相色谱法,研究了以50 mg/kg (体质量)的剂量在口灌给药条件下,复方磺胺甲噁唑(甲氧苄啶.磺胺甲噁唑=1.5)在松浦镜鲤Cyprinus carpio Songpu体内的药动学与残留消除规律。结果表明：单次给药后,甲氧苄啶在血浆和肌肉中的药时关系符合一级吸收一室开放模型,在肝胰脏和肾中的药时关系符合一级吸收二室开放模型,磺胺甲噁唑在血浆、肌肉、肝胰脏和肾中的药时关系符合一级吸收二室开放模型;每天一次连续5 d给药后,甲氧苄啶于第15天开始低于0·05 mg/kg,磺胺甲噁唑于第9天开始低于0·10 mg/kg。研究表明,在本试验条件下,建议休药期不低于15 d。     

钻井噪声与振动对鲤鱼生长的影响

采用剪标记法，现场模拟测定了钻井噪声与振动对鲤鱼生长的影响，研究结果表明，钻井噪声与振动对鲁鱼生长有显著影响，其临界等效噪声级和振动级约为８３．９dB和８９.7dB,影响域径约为９m，污染持续时间，体重和群居行为等生态因素，能显著改变钻井噪声与振动对鲤鱼生长的污染效应，由在污染消失后鲤鱼生长率能迅速恢复，这说明钻井噪声与振动鲤鱼的生长的污染效应，由于在污染消失后鲤鱼生长率能迅速恢复，这说明钻井噪声与振动对鲤鱼的影响机制可能是合理的。     

喹乙醇诱导鲤肝细胞凋亡的研究

细胞凋亡(apoptosis)是受基因控制的细胞自主的有序死亡。凋亡细胞的形态特征主要表现为细胞核的染色质浓缩,边移,聚集在核膜下,进而核发生裂解,核碎片与细胞碎片有单层膜包裹形成凋亡小体(apoptosisbody)[1]。目前已发现某些物理因素(辐射、高温等)、细胞因子、病毒感染和     

Growth testing of two breeds of common carp, Cyprinus carpio L. (Hungarian mirror and Třeboň scaly carp), in ponds with low and high stocking density

The growth of two breeds of common carp, Cyprinus carpio L., was tested in ponds under the climatic conditions of South Bohemia. T?eboň scaly carp (TR) and Hungarian mirror carp (M2) were kept in both low and high stocking densities during the second growing season and then stocked together for communal testing during the third growing season. Before the communal testing, the mean initial weights of fish from low‐ and high‐density stocks differed significantly (374.1 vs. 227.7 g for the TR breed and 766.7 vs. 317.3 g for the M2 breed respectively, P<0.01). After communal testing, mean weights of fish from low‐ and high‐density stocks gained 761.8 vs. 543.8 g for the TR breed and 1339.7 vs. 706.7 g for the M2 breed respectively. These observed weights were also significantly different (P<0.01). However, the test of corrected weight gain, i.e. gain not related to the initial weight of fish, revealed insignificant differences (P>0.01) between the weight gains after correction, i.e. the effect of different initial weights was successfully eliminated. These results seem to confirm the applicability of this method for the assessment of growth of purebred common carp under the climatic conditions of Central European fish farms.