鸡白细胞介素-6原核表达及双抗夹心ELISA方法的建立

应用分子生物学技术原核表达ChIL-6，以ChIL-6重组蛋白为免疫原，按免疫程序分别制备兔抗和鼠抗IL-6重组蛋白的多克隆抗体。应用此抗体建立双抗夹心EL-ISA方法后，为了优化此方法本试验对该方法的最佳试验条件、标准曲线、重复性和初步应用进行确定。结果显示，经SDS-PAGE和Western-blot分析，表明ChIL-6在大肠杆菌中正确表达，为了建立检测ChIL6的双抗夹心ELISA方法，本试验应用表达的重组蛋白制备兔抗和鼠抗多克隆抗体。建立的双抗夹心ELISA方法最佳反应条件为包被抗体的质量浓度为50mg／L，4℃过夜；酶标二抗稀释度为1：400，37。C1h；应用该方法检测感染金黄色葡萄球菌后的ChIL-6，其结果与本实验室之前应用人的ELISA试剂盒检测的结果相似。结果表明，本试验建立的检测ChlL-6的双抗夹心ELISA方法可用于临床。     

GLIS6下用EXCEL数据实现读者信息批量快速导入的方法

为新生读者办理借阅证是高校图书馆流通管理的一个基础环节,如何短时间内为学生办好借阅证,是摆在各家图书馆面前一项急需解决的问题。通过在实践中摸索,找到了一种利用Excel数据,实现读者信息批量快速导入SQL数据库的方法,从而极大地提高了办理借阅证的效率。     

山丹马6-磷酸葡萄糖脱氢酶及葡萄糖磷酸异构酶的多态性研究

用淀粉凝胶电泳及琼脂覆盖技术对山丹马的血液红细胞6磷酸葡萄糖脱氢酶和葡萄糖磷酸异构酶的电泳变异进行了测定。在6磷酸葡萄糖脱氢酶座位发现了3种表现型,即FF,FD和FS,其频率分别为0.958,0.021和0.021。在葡萄糖磷酸异构酶座位发现了两种表现型,即II和FI,其频率分别为0.771和0.229。等位基因频率直接通过表型计算出:PGDF为0.979,PGDD为0.0104,PGDS为0.0104;GPIF为0.1146,GPII为0.8854。亲子关系排除概率在6PGD和GPI座位点分别为0.1009和0.0912。两座位的个体识别概率分别为0.081和0.353。     

Effect of pyrethroids on carbohydrate metabolic pathways in common carp,Cyprinus carpio

The activity levels of succinate dehydrogenase (SDH) and glucose‐6‐phosphate dehydrogenase (G6PD) were assessed in various tissues of Cyprinus carpio var communis which had been exposed to lethal concentrations of group‐II pyrethroids (deltamethrin, cypermethrin, fenvalerate and fluvalinate) for a period of 72 h. The results indicated a steady decrease in SDH activity with a concomitant increase in G6PD activity. The decreased SDH activity indicated inhibition of SDH at mitochondrial level and the increased G6PD activity an enhancement of an alternative pathway of carbohydrate metabolism, viz the hexose monophosphate shunt (HMP) or pentose phosphate pathway as a biochemical adaptation to overcome the toxic stress. © 2001 Society of Chemical Industry     

Effects of N-methyl-D, L-aspartate on secretion of growth hormone-releasing hormone from the boar hypothalamic-preoptic area and median eminence in vitro

N-methyl-D, L-aspartate (NMA) elicited secretion of growth hormone (GH)-releasing hormone from both the hypothalamic-preoptic area and the median eminence that were collected from boars. We suggest that the previously described increase in GH secretion that follows peripheral treatment of swine with NMA is attributable, at least in part, to NMA-stimulated secretion of GH-releasing hormone from the central nervous system.     

Sequences of the 3′ Halves of the Genomes of Barley Yellow Dwarf Virus-PAV cpA Isolates that Vary in Symptom Severity

Barley yellow dwarf virus (BYDV)-PAV isolates from USA have been separated into two distinct clusters (Chay et al. (1996) Virology 219: 57–65; Chay et al. (1996) Phytopathology 86: 370–377). Following this finding we have shown that BYDV-PAV is divided into two groups cpA and cpB based on their coat protein gene sequence, and distinct host preferences (Mastari et al. (1998) Phytopathology 88: 818–821). We have sequenced the complete 3 half of the genomes of two lethal and two mild cpA isolates and compared them with those of several known PAV cpA isolates to assess variability and locate potential determinants of severity. Open reading frames (ORFs) 3, 4, 5, 6 and the 3 untranslated regions had different percent homologies between isolates: ORF5 (92–97%), ORF3 (88–98%) 3-translational enhancer (87–100%) ORF4 (85–99%), 3 untranslated region (72–97%) and ORF6 (61–99%). In contrast to the mild isolates, the field-lethal isolates (FHv1 and FHv2) fell into the same cluster, regardless of the genomic region analysed. The isolates FHv1 and FHv2 differed from mild isolates by eight amino acid substitutions in ORFs 3 and 4, and insertions in ORF5. Four amino acid substitutions in the 17-kDa protein encoded by ORF4 caused a change in local net charge in the field-lethal isolates. Two insertions of four amino acids were identified in the C-terminal half of ORF5 of the field-lethal isolates, but were not present systematically in all lethal isolates analysed. The potential relationships of these differences in predicted amino acid sequences to disease severity are discussed.     

Alteration of serum interleukin-6 and tumor necrosis factor-α after ischemic stimulation of coronary artery in PTCA

AIM: Inflammatory responses play an important role in the post- percutaneous transluminal coronary angioplasty (PTCA) restenosis and has been demonstrated occuring immediately after PTCA. Interleukin-6(IL-6) and tumor necrosis factor-α(TNF-α) are the main inflammatory cytokines. We try to compare the changes in interleukin-6(IL-6) and TNF-α after PTCA in the patients with and without collateral circulation to probe into the pathogenesis of early inflammatory response. METHODS: The extent of myocardial ischemia induced by balloon inflation was quantified by a scoring system referring to the Leaman coronary score. The IL-6、TNF-α levels of coronary heart disease group and control group before and after PTCA are calculated. RESULTS: The concentrations of IL-6 and TNF-α were (9.592±1.847) ng/L and (26.959±1.967) ng/L, respectively, and were significantly increased 4 hours after PTCA. CONCLUSION: IL-6 and TNF-α are sensitive indicators of the early inflammatory response after PTCA. Ischemia scores reflected the extent of ischemia reperfusion injury during PTCA. Collateral circulation decreased the early inflammatory response after PTCA.     

Flowering-pattern and yield components at inflorescence nodes of snap bean as affected by irrigation and plant density

This paper reports the results of a 2 × 2 factorial experiment on bush snap beans ‘Oregon 1604’. The treatments were 2 contrasted irrigation regimes and 2 contrasted plant densities, and were applied in 1978 and repeated in 1979. Data were collected on the number of flowers and pods, and pod size, at each node of the terminal inflorescence (6-T) of the main stem, and at each node of the oldest inflorescence (2-A) at Node 2. High and low plant densities were 45 and 18 plants m^?2 in 1978 and 54 and 33 plants m^?2 in 1979. High temperatures, frequently above 32°C, prevailed during bloom and pod development in 1978, but for the most part occurred only during the week prior to bloom in 1979. Inflorescences 6-T and 2-A usually formed 4 and 3 RN's, respectively, in 1978 and 3 and 2 RN's in 1979. The flowers at the proximal nodes of each inflorescence all opened within a few days of one another (duration of flowering at proximal nodes between 3 and 5 days); the flowering-periods of adjacent nodes overlapped, and the flowering period increased acropetally within the inflorescence (duration of flowering at distal nodes between 7 and 13 days). In general, number of flowers, pods formed, pods harvested and percent set decreased acropetally within each inflorescence. The rate of acropetal decline was lessened by high irrigation or low plant density. In both years, high irrigation increased the percent set of all RN's of the 2-A inflorescences, but few other consistent effects between years were observed. The 2 most proximal RN's together produced 93% or more of the yield of each inflorescence. High irrigation significantly increased the total number of pods harvested from these RN's of inflorescences 6-T and 2-A, and low density had a similar effect on 2-A.     

Present Concepts on the Inflammatory Modulators with Special Reference to Cytokines*

The pro- and anti-inflammatory cytokines create a network of interactions between cells that lead to both stimulatory and inhibitory responses that maintain an effective homeostatic regulation. The anti-inflammatory cytokines are a family of peptides that modulate the pro-inflammatory cytokine response. Cytokines act in concert with non-cytokine mediators, such as prostaglandin E₂, glucocorticosteroids, lipocortins, and catecholamines. This review highlights new developments in our understanding of the pathophysiology of inflammation and gives an example of a more recent approach to the modulation of acute systemic inflammatory disorders: activation of ₂-adrenergic receptors on macrophages. In this respect the potent ₂-adrenergic agonist clenbuterol seems of therapeutic interest.     

猪ERK6基因编码区的克隆及组织表达谱分析

利用RT-PCR方法扩增了猪ERK6基因编码区的序列，将扩增产物与pMD18-T载体连接，构建了重组质粒；重组质粒经PCR、酶切鉴定后进行测序；采用Northern杂交和半定量RT-PCR方法在猪不同部位骨骼肌中分析了ERK6基因转录本的个数、大小及组织表达谱。结果显示，所克隆的猪ERK6基因片段长1113bp，含有1个1104bp的开放阅读框（ORF），该ORF编码367个氨基酸；该基因与已报道的人ERK6基因核苷酸序列相似性为90％；猪ERK6基因在肌肉组织中只有一个转录本，大小约1．5kb。此基因在骨骼肌中的表达量最高，心、子宫次之，卵巢最低；而在脂肪、胃、肝、脾、肺、肾、十二指肠和胰腺中未见表达。结果表明，猪ERK6基因与已报道的小鼠和人ERK6基因一样，主要在骨骼中特异表达。