Analysis on Polymorphisms of Three CNV Regions in Five Pig Breeds

The paper was aimed to investigate the polymorphism of copy number variation (CNV) in different pig breeds.Three CNV regions of CNVR91,CNVR92 and CNVR143 were chosen from the porcine SNP60 chip genotyping results.The polymorphisms of three CNVs were determined by Real-time quantitative PCR method,taking five pig breeds as samples,including Yorkshire pig,Xiang pig,Kele pig,Nuogu pig and Rongchang pig breeds.The results showed that the dominant status of CNVR91 was loss in Xiang pig,while it was normal in other four pig breeds.The major type of CNVR92 was deletion in Xiang pig,Yorkshire pig,Kele pig and Rongchang pig breeds,with a high normal percent in Nuogu pig.For CNVR143,the dominant event was gain in Xiang pig and Nuogu pig breeds,but it was not diverse in other three pig breeds.These results indicated that three CNV regions emerged with polymorphism in five pig breeds,which might have effects on gene expression in CNV regions and physiological function by dosage effect especially in Xiang pig,Nuogu pig and Kele pig breeds.     

猪瘟病毒石门株在ST细胞连续传代后E0基因变异分析

为了解猪瘟病毒(CSFV)石门(Shimen)株连续传代后变异情况,根据已发表的CSFV E0基因序列(AF092448.2),设计合成引物,以在ST细胞中连续传代培养的CSFV石门株细胞毒总RNA为模板,每5代通过RT-PCR方法扩增病毒的E0基因,测定其核苷酸序列和氨基酸序列,用DNA Star软件分析比较原代、5代、10代、15代、20代、25代接毒细胞中CSFV E0基因的变异性。结果显示,接种病毒第10代的病毒E0基因在630核苷酸位点出现变异,第15代病毒在632核苷酸位点出现变异。本研究通过分子生物学方法发现CSFV Shimen株在ST细胞连续传代过程中E0基因能保持遗传的稳定性。     

A morphological analysis of the skull size and shape of Kerivoulinae (Chiroptera: Vespertilionidae) from Vietnam

Southeast Asia is a region of high biodiversity, containing species of plants and animals that are yet to be discovered. In this region, bats of the subfamily Kerivoulinae are widespread and diverse with six species recorded in Vietnam. However, the taxonomy of the Kerivoulinae in Asia is complicated. In our study, we used diagnostic characters and multivariate analysis to determine morphological differences between the genera Kerivoula and Phoniscus. We showed that the two genera are distinguishable by the size of second upper incisors, the shape of skull, nasal sinus, canines, second upper and lower premolars. In addition, the two genera can be osteometrically separated by measurements of the braincase height, interorbital width and shape of anterior palatal emargination. Our data clearly revealed the morphological variations in the skull shape of Kerivoula hardwickii in Vietnam. This suggests a possible separation into three morphotypes, representing cryptic species supported by statistical differences with wide variation in skull shape, size and teeth. These results demonstrated Kerivoula hardwickii can be separated three subspecies, and the result will serve as the basis for the future assessment and classification of this group in Southeast Asia.     

"已实现"波动率理论研究与评价

"已实现"波动率是一种全新的金融波动率测量方法。"已实现"波动率在理论上没有测量误差的无偏估计量,在实证建模方面比其他模型更易于估计参数,同时最优频率的选取对于"已实现"波动率的测量精确度是很重要的。     

县域耕地土壤养分时空变异特征研究

土壤养分空间变异研究是精准农业实施的前提,对提高粮食作物单位面积产量和保护农业生态环境具有重要作用。为掌握陕西渭北旱塬区土壤养分时空变异规律,综合空间自相关和重心模型,分析1980—2007年、2007—2017年白水县耕地土壤有机质(SOM)、速效磷(AP)和速效钾(AK)的时空变异特征。结果表明：(1)研究区土壤SOM的平均含量分别增加了1.29、3.22 g·kg^-1,AP的平均含量分别增加了6.89、24.45 mg·kg^-1,仅有AK的平均含量呈现出先减少后增加的趋势。(2)三期耕地土壤养分的变异系数范围为30.2%～90.8%,属于中等变异。1980—2017年,耕地土壤SOM、AP和AK的全局Moran’s I指数均降低,空间结构性减弱,随机因素作用增强。(3)土壤SOM、AP和AK含量均有不同程度的增加,土壤SOM含量总体呈北低南高分布,土壤AP、AK含量分布情况较为一致,呈东高西低。(4)土壤SOM含量重心在东南方向发生偏移,AP、AK含量重心在东北方向发生偏移。土壤养分标准差椭圆空间分布格局同研究区分布方向一致,转角呈现“...     

Using genetic tools to inform conservation of fragmented populations of Asian elephants (Elephas maximus) across their range in China

A herd of 15 Chinese elephants attracted international attention during their 2021 northward trek, motivating the government to propose establishment of an Asian elephant national park. However, planning is hampered by a lack of genetic information on the remaining populations in China. We collected DNA from 497 dung samples from all 5 populations encompassing the entire range of elephants in China and used mitochondrial and microsatellite markers to investigate their genetic and demographic structure. We identified 237 unique genotypes (153 females, 84 males), representing 81% of the known population. However, the effective population size was small (28, range 25–32). Historic demographic contraction appeared to account for low haplotype diversity (H_d = 0.235), but moderate nucleotide and nuclear diversity (π = 0.6%, H_e = 0.55) was attributable to post-bottleneck recovery involving recent population expansion plus historical gene exchange with elephants in Myanmar, Lao PDR, and Vietnam. The 5 populations fell into 3 clusters, with Nangunhe elephants differing consistently from the other 4 populations (F_ST = 0.23); elephants from Mengyang, Simao, and Jiangcheng belonged to a single population (henceforth, MSJ), and differed from the Shangyong population (F_ST = 0.11). Interpopulation genetic variation reflected isolation by distance and female-biased dispersal. Chinese elephants should be managed as 2 distinct units: Nangunhe and another combining Shangyong and MSJ; their long-term viability will require restoring gene flow between Shangyong and MSJ, and between elephants in China and neighboring countries. Our results have the potential to inform conservation planning for an iconic megafaunal species.     

中国沙棘的性状变异与演化趋势

在中国沙棘天然分布区的主轴线上,自西南向东北,选择5个有代表性的地段,用样地调查和统计分析方法.研究了中国沙棘主要性状变异及性状间的组配情况;比较了不同地段的异同;统计了各主要经济性状的变异参数;探索了几个主要性状的演化趋势。认为中国沙棘乃是多态型的种群系统,即存在很大幅度的随机变异,也存在着地理种群间的差异。种群变异的主要特征是各主要性状出现频率随生态——地理条件而递变,但群内变异幅度小,而群间差异显著。     

小麦品种无性系变异改良技术研究

针对晋阳345和晋麦73两个定型品种存在抗寒性较差的缺点,对其幼胚愈伤组织进行了4种处理:1、愈伤组织在MD培养基上继代2～3次;2、愈伤组织在紫外灯下约10cm照射2h;3、愈伤组织在低温2℃和常温25℃下交替培养30d;4、愈伤组织在紫外灯下照射2h后,再在低温2℃和常温25℃下交替培养30d。结果表明,4个处理后代R2主要农艺性状的变异率差异显著,且紫外灯照射幼胚愈伤组织的变异率比低温2℃和常温25℃交替处理幼胚愈伤组织高,其后代各种农艺性状的变异范围较大,变异率较高。但后者有利于改良抗寒性较差的定型小麦品种。     

Saturation and Parameter Variation Influence Precision of Observer

The authors analyze the influence in vector controlled induction machine caused by parameter variation and main flux saturation affects speed estimation of adaptive state observer. According to simulation of the modified scheme with saturated induction motor model, it is proved that when the vector control scheme operates in field weakening region, the main flux saturation will obviously influence precision of speed observer and bring great error of speed estimation. Thereby, the main flux saturation should be compensated to improve performance of the vector control system.