饲粮精料水平和蛋氨酸铬添加剂量对舍饲滩羊生长性能、屠宰性能、肉品质和脂肪沉积的影响

本试验旨在探究饲粮精料水平和蛋氨酸铬(Cr-Met)添加剂量对舍饲滩羊生长性能、屠宰性能、肉品质和脂肪沉积的影响。采用2×3双因素试验设计,2个因素分别为饲粮精料水平和Cr-Met添加剂量,其中饲粮精料水平分别设为35%(低精料饲粮,饲粮精粗比为35∶65)和55%(高精料饲粮,饲粮精粗比为55∶45),Cr-Met添加剂量分别设为0、0.75和1.50 g/(d·只)。将60只雄性滩羊羔羊[平均体重为(21±1)kg]随机分配到6个试验组,每组10只。试验期为80 d,其中预试期15 d,正试期65 d。结果显示:1)与低精料饲粮组相比,高精料饲粮组滩羊的平均日增重、屠宰率、背膘厚度、肌内脂肪含量和后腿肉比重显著增加(P<0.05),但料重比与肌肉蒸煮损失、剪切力、pH、亮度(L*)值、红度(a*)值和黄度(b*)值则显著降低(P<0.05)。2)低精料饲粮组滩羊背膘厚度、皮下脂肪厚度、肌内脂肪含量和后腿肉比重随Cr-Met添加剂量的增加而线性降低(P<0.05),但肌肉pH则线性升高(P<0.05)。3)高精料饲粮组滩羊肋肉比重、腰肉比重以及肌肉剪切力和pH随Cr-Met添加剂量的增加而线性升高(P<0.05),而肌内脂肪含量和肌肉a*值则线性降低(P<0.05)。综合本试验测定指标,建议在滩羊养殖中选择精粗比为55∶45的高精料饲粮,Cr-Met的适宜添加剂量为1.50 g/(d·只),且不建议在精粗比为35∶65的低精料饲粮中添加Cr-Met。     

智慧物流在饲料企业管理中的应用研究

饲料行业的竞争日益激烈,其需要学会使用现代科技提升竞争力,智慧物流就是这些技术之一。智慧物流可以提升饲料企业对原材料和产品在物流过程中的管控。本文试图探索智慧物流如何提升饲料企业的管理。通过智慧物流,饲料企业可以改进生产管理的流程和模式,加强采购管理中的风险管理,追踪产品售后的流向和信息,提升企业的产品质量,保证企业财务数据的真实性和可靠性。     

Exogenous application of allelopathic water extracts helps improving tolerance against terminal heat and drought stresses in bread wheat (Triticum aestivum L. Em. Thell.)

The allelopathic water extracts (AWEs) may help improve the tolerance of crop plants against abiotic stresses owing to the presence of the secondary metabolites (i.e., allelochemicals). We conducted four independent experiments to evaluate the influence of exogenous application of AWEs (applied through seed priming or foliage spray) in improving the terminal heat and drought tolerance in bread wheat. In all the experiments, two wheat cultivars, viz. Mairaj‐2008 (drought and heat tolerant) and Faisalabad‐2008 (drought and heat sensitive), were raised in pots. Both wheat cultivars were raised under ambient conditions in the wire house till leaf boot stage (booting) by maintaining the pots at 75% water‐holding capacity (WHC). Then, managed drought and heat stresses were imposed by maintaining the pots at 35% WHC, or shifting the pots inside the glass canopies (at 75% WHC), at booting, anthesis and the grain filling stages. Drought stress reduced the grain yield of wheat by 39%–49%. Foliar application of AWEs improved the grain yield of wheat by 26%–31%, while seed priming with AWEs improved the grain yield by 18%–26%, respectively, than drought stress. Terminal heat stress reduced the grain yield of wheat by 38%. Seed priming with AWEs improved the grain yield by 21%–27%; while foliar application of AWEs improved the grain yield by 25%–29% than the heat stress treatment. In conclusion, the exogenous application of AWEs improved the stay green, accumulation of proline, soluble phenolics and glycine betaine, which helped to stabilize the biological membranes and improved the tolerance against terminal drought and heat stresses.     

Replication of neurovirulent equine herpesvirus type 1 (EHV-1) in CD172a+ monocytic cells

Equine herpesvirus type 1 (EHV-1) is responsible for respiratory disorders, abortion and myeloencephalopathy (EHM) in horses. Two pathotypes of EHV-1 strains are circulating in the field: neurovirulent (N) and non-neurovirulent (NN). For both strains, CD172a⁺ monocytic cells are one of the main carrier cells of EHV-1 during primary infection, allowing the virus to invade the horse’s body. Recently, we showed that EHV-1 NN strains showed a restricted and delayed replication in CD172a⁺ cells. Here we characterize the in vitro replication kinetics of two EHV-1 N strains in CD172a⁺ cells and investigate if the replication of these strains is similarly silenced as shown for EHV-1 NN strains. We found that EHV-1 N replication was restricted to 7–8% in CD172a⁺ cells compared to 100% in control RK-13 cells. EHV-1 N replication was not delayed in CD172a⁺ cells but virus production was significant lower (10^3.0 TCID₅₀/10⁵ inoculated cells) than in RK-13 cells (10^8.5 TCID₅₀/10⁵ inoculated cells). Approximately 0.04% of CD172a⁺ cells produced and transmitted infectious EHV-1 to neighbour cells compared to 65% of RK-13 cells. Unlike what we observed for the NN strain, pretreatment of CD172a⁺ cells with histone deacetylases inhibitors (HDACi) did not influence the replication of EHV-1 N strains in these cells. Overall, these results show that the EHV-1 replication of N strains in CD172a⁺ cells differs from that observed for NN strains, which may contribute to their different pathogeneses in vivo.     

Determination of critical nitrogen dilution curve based on leaf area index for winter wheat in the Guanzhong Plain,Northwest China

Excessive use of nitrogen(N) fertilizers in agricultural systems increases the cost of production and risk of environmental pollution. Therefore, determination of optimum N requirements for plant growth is necessary. Previous studies mostly established critical N dilution curves based on aboveground dry matter(DM) or leaf dry matter(LDM) and stem dry matter(SDM), to diagnose the N nutrition status of the whole plant. As these methods are time consuming, we investigated the more rapidly determined leaf area index(LAI) method to establish the critical nitrogen(N_c) dilution curve, and the curve was used to diagnose plant N status for winter wheat in Guanzhong Plain in Northwest China. Field experiments were conducted using four N fertilization levels(0, 105, 210 and 315 kg ha-1) applied to six wheat cultivars in the 2013–2014 and 2014–2015 growing seasons. LAI, DM, plant N concentration(PNC) and grain yield were determined. Data points from four cultivars were used for establishing the N_c curve and data points from the remaining two cultivars were used for validating the curve. The N_c dilution curve was validated for N-limiting and non-N-limiting growth conditions and there was good agreement between estimated and observed values. The N nutrition index(NNI) ranged from 0.41 to 1.25 and the accumulated plant N deficit(N_(and)) ranged from 60.38 to –17.92 kg ha~(-1) during the growing season. The relative grain yield was significantly affected by NNI and was adequately described with a parabolic function. The N_c curve based on LAI can be adopted as an alternative and more rapid approach to diagnose plant N status to support N fertilization decisions during the vegetative growth of winter wheat in Guanzhong Plain in Northwest China.     

Identification and quantification of anthocyanins in different coloured cultivars of ornamental kale (Brassica oleracea L. var. acephala DC)

Anthocyanins are responsible for the colour of many fruits, vegetables, flowers, and coloured-leaved trees. Ornamental kale (Brassica oleracea L. var. acephala DC) is widely cultivated for its colourful inner leaves. To investigate the relationship between the degree of colouration and anthocyanin distribution, content, and composition in ornamental kale, the authors studied the pigment characteristics of five cultivars with different coloured leaves (white, pink, red, purple, and purple-black). Microscopy observation, spectrophotometer, and high-performance liquid chromatography-mass spectrometry (HPLC-MS) analysis of fresh inner leaves revealed that pink, red, and purple colourations were associated with high levels of anthocyanin, while purple-black was the result of the combination of anthocyanins and chlorophyll. In the coloured cultivars, anthocyanins were abundant mainly in the first and second cell layers below the epidermis in both the hypocotyls and inner leaves. No anthocyanin was found in the white-leaved phenotype cultivar. Anthocyanin content increased as leaf colour deepened from pink, red, to purple cultivars, which had little chlorophyll and carotenoid. The authors identified eight anthocyanins in the four coloured cultivars, including one non-acylated, four monoacylated, and three diacylated cyanidin glycosides. Cyanidin-3-(sinapoyl)(feruloyl)-diglucoside-5-glucoside was the most abundant anthocyanin in the four coloured cultivars followed by cyanidin-3-(sinapoyl)-diglucoside-5-glucoside. The analysis of anthocyanin accumulation characterisation provides important information on evaluating colouration patterns in coloured plants, and will be helpful for breeding desired leaf colours in ornamental kale.     

杏鲍菇秋葵咀嚼片配方优化与质量标准研究

为了优选杏鲍菇秋葵咀嚼片配方,以杏鲍菇粗多糖、杏鲍菇超细粉为主要原料,采用全粉末直接压片技术,选取片重差异、崩解时限、硬度及脆碎度的综合评定值为评价指标,通过响应面法确定最佳配方,并制定产品的质量指标。结果表明,杏鲍菇秋葵咀嚼片的最佳配方为杏鲍菇粗多糖38%、杏鲍菇超细粉49%和黄秋葵超细粉13%,制成的咀嚼片表面光滑、色泽均匀。综上,采用多指标综合评分法优化杏鲍菇秋葵咀嚼片配方是可行的,所得工艺配方稳定可靠,这为我国咀嚼片剂工艺创新及其产业化应用研究提供了新思路。     

不同LED光质对紫甘蓝幼苗生长的影响

以紫甘蓝品种‘紫辉甘蓝’为试材,在南方塑料大棚内密闭式光照植物培养架中,采用新型LED光源研究了不同光质对紫甘蓝幼苗生长的影响,试验共设置6个处理:R(红)、B(蓝)、8R2B(红光∶蓝光=8∶2)、5R5B(红光∶蓝光=5∶5)、2R8B(红光∶蓝光=2∶8)和荧光灯(对照CK)。结果表明:(1)在8R2B处理下,紫甘蓝幼苗地下部鲜重、地上部鲜重、单株鲜重、株高、根体积、根总表面积和根投影面积均最大;(2)与CK相比,不同光质处理均提高了幼苗叶片叶绿素a、叶绿素b和类胡萝卜素的含量,以2R8B处理最高;(3)与CK相比,除R处理的幼苗可溶性蛋白质含量降低外,其它处理均有助于幼苗可溶性蛋白质含量的积累,以8R2B处理最高;(4)可溶性糖含量以R处理最高;(5)与CK相比,各光质处理均显著降低了幼苗MDA含量,以B处理效果最显著,8R2B和5R5B次之;(6)各光质处理对紫甘蓝幼苗叶片脯氨酸含量的影响与CK相比无显著性差异。     

氮肥不同用量对北方粳稻群体质量及品质的影响

氮是植物体内氨基酸、蛋白质、核酸、辅酶以及光合色素分子等的组成成分,因此,氮的营养状况与许多生理过程密切相关。针对水稻氮肥用量过高这一突出问题,本试验研究了氮肥施用总量对北方粳稻产量及其构成因素、叶面积指数、干物质积累、群体库容量以及品质特征等方面的影响,进一步明确不同氮肥施用量条件下北方粳稻产量和品质形成的调控机制。结果表明,施氮量的增加对叶面积指数、光合势及群体生长率有促进作用,随着施氮量的增加,水稻最高茎数、最高分蘖数、分蘖穗数和分蘖穗比例有逐渐增加的趋势;增加施氮量会导致整精米率、蛋白质含量上升,精米白度、食味值、峰值粘度、最低粘度、崩解值、最终粘度值降低,但不同施氮量对直链淀粉含量影响不大。