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91.
LI Mei-dong 《山东饲料》2014,(21)
This paper gives a brief introduction of Kate Chopin and his famous work The Story of an Hour [1].By analyzing the psychological change of the protagonist Mrs. Mallard and her reactions to her husband'... 相似文献
92.
Damián Escribano Ana M. Gutiérrez María Fuentes-Rubio José J. Cerón 《Veterinary journal (London, England : 1997)》2014,199(3):355-359
Salivary chromogranin A (CgA) is considered to be a biomarker of activation of the sympatho-adrenomedullary system, and has recently been proposed as a useful indicator of the acute stress response in pigs. The aim of the present study was to determinate whether salivary CgA concentrations in healthy growing pigs exhibits any circadian pattern during the daytime, and to evaluate its stability under different storage conditions. A total of 80 pigs (40 in spring and another 40 in autumn) of two different ages and genders were used. To establish the circadian pattern, saliva samples were collected at 07.00, 11.00, 15.00 and 19.00 h on two consecutive days. Pooled samples were used for the stability study and were measured on the day of sampling and periodically for up to 360 days later. Samples were stored at 4 °C, ?20 °C or ?80 °C and the effect of repeated freezing and thawing was also evaluated.No circadian pattern was detected for salivary CgA in either season and there were no significant effects of gender or age. However, mean salivary CgA concentrations were significantly higher (P < 0.0001) in the pigs sampled in autumn, compared to those sampled in the spring. Short term storage at 4 °C is recommended for up to 2 days, whereas frozen samples can be stored for 1 year at ?20 °C or ?80 °C, without substantial reduction in CgA values. In addition, samples can be frozen and thawed up to seven times without significant loss of the biomarker. 相似文献
93.
弓形虫可感染多种动物引起严重的弓形虫病,为了对不同宿主感染的弓形虫株基因型差异及不同基因型弓形虫虫株的致病力差异进行进一步研究,弓形虫基因型的分析多采用限制性片段长度多态性PCR(PCR-RFLP)、随机扩增多态性DNA、多位点PCR-RFLP分析、DNA序列分析和微卫星DNA序列分析等技术,扩增位点多选择SAG1、SAG2、SAG3、BTUB和GRA6等遗传标记。传统的弓形虫基因型主要有3个,随着基因型研究的不断深入,越来越多的基因型被发现。本文就弓形虫基因分型研究进展进行讨论分析。 相似文献
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《国际水土保持研究(英文)》2022,10(3):470-486
This study was performed to examine the separate and simultaneous influence of predictive models’ choice alongside sample ratios selection in soil organic matter (SOM). The research was carried out in northern Morocco, characterized by relatively cold weather and diverse geological conditions. The dataset herein used accounted for 1591 soil samples, which were randomly split into the following ratios: 10% (~150 sample ratio), 20% (~250 sample ratio), 35% (~450 sample ratio), 50% (~600 sample ratio) and 95% (~1200 sample ratio). Models herein involved were ordinary kriging (OK), regression kriging (RK), multiple linear regression (MLR), random forest (RF), quantile regression forest (QRF), Gaussian process regression (GPR) and an ensemble model. The findings in the study showed that the accuracy of SOM prediction is sensitive to both predictive models and sample ratios. OK combined with 95% sample ratio performed equally to RF in conjunction with all the sample ratios, as the latter did not show much sensitivity to sample ratios. ANOVA results revealed that RF with a ~10% sample ratio could also be optimum for predicting SOM in the study area. In conclusion, the findings herein reported could be instrumental for producing cost-effective detailed and accurate spatial estimation of SOM in other sites. Furthermore, they could serve as a baseline study for future research in the region or elsewhere. Therefore, we recommend conducting series of simulation of all possible combinations between various predictive models and sample ratios as a preliminary step in soil organic matter prediction. 相似文献
95.
粒重稳定性是小麦稳产性的重要评价指标。为了发掘小麦在遭受虫害等减源逆境伤害时影响粒重稳定性的QTL,以小偃81和西农1376杂交衍生的重组自交系(RILs)群体为试验材料,设早播和晚播两个播期,对减源处理下该群体的粒长、粒宽和千粒重进行表型分析,同时计算减源处理与对照处理的表型比值,进而估测发育稳定性,并利用50K芯片构建的遗传连锁图谱进行QTL定位。结果表明,与对照处理相比,减源处理下RIL群体的粒长、粒宽和千粒重均显著下降。采用完备复合区间模型对不同处理的表型值、表型比值分别进行QTL定位,共检测到18个QTL,分布于2A、3A、3B、3D、4B、4D和5D染色体上,其中有8个QTL在减源处理和对照处理中均表达, Qgl.nwafu-5D.3和 Qkgw.nwafu-5D均在早播对照处理和晚播减源处理中被检测到,平均可解释表型变异的8.73%和8.19%,为稳定QTL,且 Qgl.nwafu-5D.3也在早播减源处理中被检测到,推测这2个稳定QTL可能为新发现的位点。控制表型比值的QTL大多与控制粒长、粒宽和千粒重的QTL区间重合,推测维持减源后粒重稳定性的QTL与控制粒长、粒宽和千粒重的位点紧密连锁或存在“一因多效”现象。 相似文献
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选择浙西低丘地区的黄筋泥田和红紫砂田2类土壤的水田与相应改旱不同年限的旱地、果园和茶园,比较研究了水田改旱后土壤团聚体组成及其稳定性的变化.结果表明,水田改旱后,土壤中>5.00 mm和>0.25 mm非水稳定性团聚体和水稳定性团聚体数量均呈现下降,土壤团聚体的水稳定性减弱,其中,水稳定性团聚体的下降比非水稳定性团聚体更为明显.水田改旱种植旱粮,土壤中>5.00 mm和>0.25 mm非稳定团聚体、水稳定性团聚体及团聚体的稳定呈持续下降;而当水田改旱种植果树和茶树时,初期土壤中>5.00 mm和>0.25 mm非稳定团聚体、水稳定性团聚体和团聚体的稳定呈明显下降,但随着时间的增加,其大团聚体逐渐恢复.改旱后土壤团聚体的变化与其有机碳和无定形氧化铁数量发生变化有关. 相似文献
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