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991.
利用14个微卫星标记对云南茶花鸡12个家系的等位基因频率、平均基因杂合度、平均多态信息含量以及家系间的亲缘关系进行了分析。结果表明:共检测到99个等位基因,每个位点的等位基因分布并不均匀,再对12个家系进行分析,将其聚为3类,一类为Z-1,Z-2,Z-3,Z-4,Z-5,Z-6,B-1,一类为Z-7,Z-8,Z-9,Z-10,矮脚系B-2自成一类,这表明了家系间的遗传相似性,同时我们对特殊的矮脚系B-2进行了相关的探讨,最后我们提出了对茶花鸡保种的相关建议。 相似文献
992.
2004~2005年上海和江苏3家猪场发生仔猪急性死亡,用ELISA鉴别试剂盒证明为猪瘟感染。用PK15细胞分离病毒,传代至第3代无细胞病变,RT-PCR扩增E2基因为阳性,扩增片段经纯化后克隆入T载体并进行序列测定。通过DNAStar软件对3株病毒的基因序列进行了比较,同时构建了系统进化树,结果表明,三株病毒分离株均扩增出269bp片段并且核苷酸序列100%同源,这3株与Alfort、Shimen株、兔化弱毒株(HCLV)和Brescia4个标准毒株核苷酸序列同源性均在90%以上,说明在江苏、上海地区分离得到的3株猪瘟病毒与流行毒株在基因序列上没有发生大的变异。 相似文献
993.
Y-M. Chang D. Gianola B. Heringstad & G. Klemetsdal 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2006,123(5):290-300
Robust threshold models with multivariate Student's t or multivariate Slash link functions were employed to infer genetic parameters of clinical mastitis at different stages of lactation, with each cow defining a cluster of records. The robust fits were compared with that from a multivariate probit model via a pseudo‐Bayes factor and an analysis of residuals. Clinical mastitis records on 36 178 first‐lactation Norwegian Red cows from 5286 herds, daughters of 245 sires, were analysed. The opportunity for infection interval, going from 30 days pre‐calving to 300 days postpartum, was divided into four periods: (i) ?30 to 0 days pre‐calving; (ii) 1–30 days; (iii) 31–120 days; and (iv) 121–300 days of lactation. Within each period, absence or presence of clinical mastitis was scored as 0 or 1 respectively. Markov chain Monte Carlo methods were used to draw samples from posterior distributions of interest. Pseudo‐Bayes factors strongly favoured the multivariate Slash and Student's t models over the probit model. The posterior mean of the degrees of freedom parameter for the Slash model was 2.2, indicating heavy tails of the liability distribution. The posterior mean of the degrees of freedom for the Student's t model was 8.5, also pointing away from a normal liability for clinical mastitis. A residual was the observed phenotype (0 or 1) minus the posterior mean of the probability of mastitis. The Slash and Student's t models tended to have smaller residuals than the probit model in cows that contracted mastitis. Heritability of liability to clinical mastitis was 0.13–0.14 before calving, and ranged from 0.05 to 0.08 after calving in the robust models. Genetic correlations were between 0.50 and 0.73, suggesting that clinical mastitis resistance is not the same trait across periods, corroborating earlier findings with probit models. 相似文献
994.
LIU Mei-lian XU Xia XIE Ping LU Jin CHEN Shu-hua ZENG Wei-min SONG Hui-ping 《园艺学报》2006,22(9):1674-1679
AIM:To investigate the influence of ovariectomy and estrogen replacement treatment on profile of gene expression in myocardium by cDNA microarray,and to characterize the targeting genes of estrogen.METHODS:cDNA microarray containing 1 400 rat cDNAs was used to study the genes differentially expressed in myocardium between sham (Ⅰ),ovariectomy (Ⅱ,OVX) and estrogen replacement treatment (Ⅲ,OVX+E2) group.Then down-regulated genes in myocardium of OVX rats were further confirmed by RT-PCR.RESULTS:177 genes were differentially expressed in myocardium between sham and OVX rats,with 91 genes up-regulated and 86 genes down-regulated in OVX rats.164 genes were differentially expressed in myocardium between OVX and OVX+E2 rats,with 113 genes up-regulated and 54 genes down-regulated in OVX rats.There were 54 genes differentially expressed in OVX compared to sham and OVX+E2.They are involved in membrane channels and transporters (18),cell receptors (9),intracellular transducers/effectors/modulator (7) and metabolism (6).Most of the genes (45) were down-regulated in OVX rats and up-regulated in OVX+E2 rats.RT-PCR test confirmed the results of cDNA microarray.CONCLUSIONS:Long-term estrogen replacement may influence the expression of genes involved in membrane channels and transporters,cell receptors,intracellular transducers/effectors/ modulator and metabolism.Long-term estrogen replacement has some beneficial effects on ionic concentration and cardiac function which partially comes from the results of influence of expression on Na+,K+-ATPase and Na+/H+ exchanger.Estrogen has an inhibitory effect on the expression of dopamine receptor,which partially clarify the myocardial protection of estrogen. 相似文献
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采用反相高效液相色谱法对蛇床子素进行分离定量,本方法的标准偏差为0.004, 变异系数0.41%;平均回收率为99.86%;线性相关系数为0.999 99。 相似文献
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