基于环保理念的“绿色图书馆”建设

国家＂十一五＂规划中,将建设节约环保型社会作为重点内容,实行限制与鼓励性政策并举的方式,制定建筑节能环保措施,提高建筑物能源利用效率和环保功能。图书馆作为公共文化建筑物,是现代文化的标志性建筑物,理应践行绿色环保理念,引导时代潮流。本文提出了＂绿色图书馆＂的构建理念,并从建筑理念、建筑模式以及建筑原则等多方面进行论述,以探究＂绿色图书馆＂在未来社会中的价值。     

绿叶蔬菜工厂化生产关键技术研究

围绕绿叶蔬菜的清洁高效生产和均衡供应需求,借鉴日本、荷兰等国家植物工厂发展的先进经验,针对绿叶蔬菜工厂化生产的关键环节和技术突破点,以小白菜的工厂化生产为例,综述了品种选择、基质研发、肥水运筹、节能生产、绿色防控、物流加工、质量管理等关键设备与技术研究进展,探索符合中国国情的有机生态型绿叶蔬菜工厂化生产的可行性。     

表达新城疫病毒融合蛋白基因禽痘病毒重组体的构建

通过RT-PCR扩增新城疫病毒F基因,利用禽痘病毒复合启动子、SV40 PolyA终止信号序列构建F基因表达盒。将F基因表达盒与loxp-GFP-loxP序列串联插入禽痘病毒重组臂,构建了禽痘病毒转移质粒载体。在脂质体介导下转染CEF,获得了带有绿色荧光信号的重组病毒rFPVFGFP。通过二次转染,利用Cre-loxP位点特异性重组将重组病毒基因组的GFP自动去除,结果获得了只含新城疫病毒F基因表达盒的重组禽痘病毒rFPVF。试验结果表明,rFPVF复制稳定,表达的F蛋白具有免疫原性,能够刺激鸡只产生抗新城疫病毒的中和抗体。     

产蛋鸡的啄羽及成因分析

从动物本身、营养及环境、饲养管理等方面分析啄羽及其成因,总结出啄羽是一种行为转向的规律。凡能引导鸡啄食的物体或环境措施都能有效降低啄羽,而营养方面只要在NRC标准内都不会额外增加鸡的啄羽(色氨酸除外)。啄羽的最终解决需要各方面专家的共同努力。     

real-time PCR将成为分子生物学研究的重要检测技术

近年来,real-tim e PCR技术作为一种敏感、快速、简便、重复性好的检测技术已广泛出现在生物学研究领域。real-tim e polym erase chain reaction(RT-PCR)是Kary M ullis在二十世纪八十年代在PCR的基础上发展革新的一项新技术,它能使小量DN A片段扩增一百万倍。这项技术推动了分子生物学技术的发展,简便的操作不仅可应用于普通实验,还可用于人类基因组工程。根据2003年调查的406位科学工作者,48%的研究人员近年来正在使用这项技术。随着使用这项技术的人数增多,各类相关的论文也相继增多。real-tim e PCR将会影响PCR技术的发展,并…     

荧光定量PCR检测小鼠卵母细胞中Mad2 mRNA的表达

目的:应用SYBR G reen I染料法建立检测Mad2 mRNA表达的实时荧光定量PCR的方法,并探讨小鼠卵母细胞减数分裂成熟中Mad2 mRNA的表达。方法:采用实时荧光定量PCR的方法,以SYBR G reen I为荧光染料,梯度稀释的重组质粒为模板制作标准曲线,并以此方法分析了小鼠卵母细胞减数分裂成熟过程中Mad2 mRNA表达的动态变化。结果:建立了实时荧光定量检测方法分析小鼠卵母细胞中Mad2 mRNA的表达,该方法灵敏、特异,扩增效率接近100%。进行了标准曲线的制作,以及对目的基因Mad2转录水平的绝对定量。结论:实时荧光定量PCR是检测基因表达水平的理想选择,在小鼠卵母细胞减数分裂成熟过程中Mad2 mRNA表达存在动态变化。     

茶树新品系福选9号的观察研究

为观察鉴定茶树新品系福选9号在重庆市巴南区的适应性及表现,以福鼎大白茶为对照品种,于2002-2004年进行了品比试验。结果表明,福选9号表现出如下特性:(1)春芽萌动特早,较对照提早11～13d,春茶产量较高。(2)育芽能力强,生长势旺盛,秋梢休止期较对照晚5～7d,全年生育期较对照长16～20d,平均单产高20.5%。(3)抗逆性强,耐寒性和抗旱性与对照相当,抗病虫性稍强。(4)发芽整齐度高,适宜机械化作业。(5)制绿茶品质优良,适制性强。     

豆瓣菜不同茎节扦插繁殖试验

以豆瓣菜葡匐茎的上,中、下三段的试材,进行不同茎段的扦插繁殖试验结果表明,中段成活率高,分枝快,适于做扦插材料。     

Spatial and temporal variability of air temperature across urban neighborhoods with varying amounts of tree canopy

Recent studies have emphasized the presence of microclimates in urban settings, but most do not have the high resolution observations necessary to understand the interactions taking place at a neighborhood scale. This study used a network of 10 identical weather stations and high resolution land cover data in Knoxville, Tennessee, to analyze the microclimates of a medium-sized city with a temperate climate. Two stations were installed in each of four urban neighborhoods in locations with varying localized tree cover, and two additional stations were installed in the center of downtown and in a nearby urban nature center. The intra-neighborhood results suggested that there is significant temperature variability within a single neighborhood based on the tree canopy density immediately surrounding a given weather station. However, the inter-neighborhood variability (differences between neighborhoods) was similar in magnitude, which suggests that the overall differences in neighborhood characteristics also have an effect on climate. Land cover at the neighborhood scale (in particular tree canopy percentages at the 500-m radii) had the highest correlation with the minimum daily temperature (Tmin) during the summer season. Maximum daily temperature (Tmax) relied most on the distance of each station from Downtown and the amount of impervious area in the 50 m surrounding each station. Tmax was also most influenced by surrounding land cover during dry conditions (a Dry Moderate air mass). Overall, highly localized impervious land cover percentages and larger-scale forested canopy were important in explaining temperature fluctuation, pointing to the importance of scale in microclimate assessments. Dry air masses enhanced the relationship between land cover and temperature during the day, while moist air masses did the same overnight. These data can be used to better inform planning strategies to build resiliency to extreme heat into urban environments by considering the influence of tree canopy.