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11.
高梁不育系Tx3197A减数分裂中染色体行为 总被引:3,自引:0,他引:3
以Tx3197B和晋粱5号为对照,对Tx3197A进行了观察。结果发现,减数分裂前期Ⅰ和中期Ⅰ无明显异常。自后期Ⅰ开始,染色体行为出现了多类型的异常现象,导致减数分裂不能正常进行,无法形成花粉粒,从而产生雄性不育。 相似文献
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Gülşen Sertkaya Marta Martini Paolo Ermacora Rita Musetti Ruggero Osler 《Phytoparasitica》2005,33(4):380-390
During the late summer-early autumn of 2002, surveys were carried out in Turkey to determine the presence of phytoplasma diseases
in fruit trees. Phytoplasmas were detected and characterized by PCR-RFLP analysis and TEM technique in stone fruit and pear
trees in the eastern Mediterranean region of the country. Six out of 24 samples, including almond, apricot, peach, pear and
plum, gave positive results in PCR assays. RFLP analysis usingSspI andBsaAI enzymes of PCR products obtained with primer pair f01/r01 enabled identification of the phytoplasmas involved in the diseases.
Stone fruit trees, including a local apricot variety (‘Sakıt’) and a pear sample, were found to be infected with European
stone fruit yellows (ESFY, 16SrX-B) and pear decline (PD, 16SrX-C) phytoplasmas, respectively. This is the first report in
Turkey of PD phytoplasma infecting pear and of ESFY phytoplasma infecting almond, apricot, myrobalan plum and peach; ESFY
phytoplasma infecting Japanese plum was previously reported.
http://www.phytoparasitica.org posting July 21, 2005. 相似文献
14.
Wanyoike Mary Wanjiru Kang Zhensheng Heinrich Buchenauer 《European journal of plant pathology / European Foundation for Plant Pathology》2002,108(8):803-810
Cytological studies were carried out to elucidate the importance of cell wall degrading enzymes (CWDE) during infection of wheat spikes by Fusarium graminearum. Scanning electron micrographs revealed that at 6–24 hours after inoculation (hai) of single spikelets with macroconidia of F. graminearum, the fungus germinated by forming several germ tubes and developed a dense hyphal network in the cavity of the spikelet. At 24–36hai, the fungus formed infection hyphae which invaded the ovary and inner surface of the lemma and palea. Transmission electron microscopical studies revealed that the fungus extended inter- and intracellularly in the ovary, lemma and rachis and caused considerable damage and alterations to the host cell walls. In different tissues of healthy and F. graminearum-infected wheat spikes the cell wall components cellulose, xylan and pectin were localized by means of enzyme-gold and immuno-gold labelling techniques. Localization of cellulose, xylan and pectin showed that host cell walls which were in direct contact with the pathogen surface had reduced gold labelling compared to considerable higher labelling densities of walls distant from the pathogen–host interface or in non-colonized tissues. The reduced gold labelling densities in the infected host cell walls indicate that these polysaccharide degrading enzymes might be important pathogenicity factors of F. graminearum during infection of wheat spikes. The results revealed that, infection and colonization of wheat spikes by F. graminearum and reactions of infected host tissue were similar to those reported for F. culmorum. 相似文献
15.
Y. Antignus M. Lapidot N. Ganaim J. Cohen O. Lachman M. Pearlsman B. Raccah A. Gera 《Phytoparasitica》1997,25(4):319-330
Received April 24, 1997; received in final form June 29, 1997. Symptoms resembling tomato spotted wilt virus (TSWV) infections
were documented among ornamental and vegetable crops in commercial greenhouses and open fields in Israel. Plants exhibiting
these symptoms were collected from January 1992 to December 1996. Among cultivated plants analyzed for TSWV by enzyme-linked
immunosorbent assay (ELISA), 19 species representing five families were found to be infected; natural infection was also recorded
in six plant species of weeds. Virus identity was characterized by host range, serology and electron microscopy. Serological
reaction with the isolates, found in Israel, using antisera from different sources as well as the sequence analysis of the
nucleocapsid gene, demonstrated that the Israeli isolates of TSWV are a member of tospovirus serogroup I, type I (BR-01 strain).
No virus transmission was found in seeds collected from virus-infected vegetable and ornamental crops. A non-radioactive molecular
probe derived from the cloned nucleocapsid isolate enables specific detection of the virus in crude sap from infected plants.
The detection of TSWV in Israel constitutes a severe potential threat to the ornamental and vegetable industry. 相似文献
16.
抗鸡传染性喉气管炎重组鸡痘病毒基因工程疫苗同居感染试验 总被引:3,自引:0,他引:3
疫苗的接触传播是疫苗免疫接种需要考虑的重要因素,为了检测重组鸡痘病毒载体疫苗水平传播的能力,对隔离条件下饲养的SPF鸡用重组鸡痘病毒基因工程疫苗接种,同时设立非免疫对照鸡,饲养期间特意延长清粪时间以增加感染的机会,1个月之后攻击传染性喉气管炎WG株强毒和鸡痘102株强毒,疫苗免疫鸡全部获得保护,而非免疫鸡则全部发病.在试验动物饲养场的自然条件下,将免疫鸡和试验对照两组鸡饲养在同一个鸡舍内,让疫苗毒的传播更接近自然条件.在每个月的攻毒试验中,对照鸡都没有获得对鸡痘和传染性喉气管炎强毒的保护.在疫苗免疫期间进行连续5个月的跟踪检测,同居未免疫鸡没有检测到抗传染性喉气管炎病毒gB抗体.这些实验结果表明抗鸡传染性喉气管炎重组鸡痘病毒基因工程疫苗不能通过接触传播. 相似文献
17.
从蔬菜产业的纵向看,批发和零售是两个重要环节,蔬菜批发价格和零售价格的走势以及它们之间的关系是各界关注的焦点。本文以北京市场为例,以番茄和黄瓜两种果类蔬菜的按日批零价格为对象,运用有限分布滞后模型和误差修正模型进行实证分析,研究蔬菜批零价格间的传导关系,并在此基础上揭示蔬菜批发价格在蔬菜价格形成中的作用。研究发现:蔬菜批零价格走势基本一致,相关系数很高;批零价格间存在批发价格向零售价格单向传导的因果关系;批零价格的传导是顺畅的,但不同种蔬菜传导的时间效应有所不同;批发价格在蔬菜价格形成中占据主导作用,当蔬菜价格受到冲击时,若零售价格过高则其会向着批发价格下调,若批发价格过高则零售价格会随之走高。 相似文献
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Guo Lixue Shi Yuzhen Li Junwen Gong Juwu Liu Aiying Shang Haihong Gong Wankui Chen Tingting Ge Qun Sun Jie Yuan Youlu 《棉花学报》2015,27(6):550-560
In this study, F2 populations from two chromosome segment substitution lines MBI7455 and MBI7358, were quantified using SSR to evaluate the parents' genotype and detect QTL related to fiber quality plus yield traits of cotton. Results show that the recurrent parent (CCRI45) hosted 96.70% and 95.60% of chromosome segment substitution in MBI7455 with 12 chromosome segments and in MBI7358 with 16 chromosome substitution segments of Gossypium barbadense, respectively. In the F2 population, the average rate of chromosome substitution of the recurrent parent (CCRI45) was 96.44%, and the average segments of chromosome substitution of Gossypium barbadense was 13.42, with an average segments of homozygous donor chromosome value of 3.90. Analysis showed 19 fiber quality-related QTL with a phenotypic variance of between 2.52%-13.11% and seven yield traits-related QTL with a range of 2.93%-11.40% phenotypic variance, resulting in a total of 26 QTL. The CSSLs could be used to detect QTL for fiber yield and quality traits, which offer an important foundation for the cotton molecular-assisted breeding. 相似文献