HOTAIR与山羊皮肤黑色素沉积的关系及体外表达规律的研究

旨在探讨山羊HOX转录反义RNA（HOTAIR）及其部分家族基因与山羊皮肤黑色素沉积的关系以及它们在黑色素细胞中的表达规律。本研究采用qRT-PCR检测HOTAIR和HOXD10、HOXC11、HOXC12基因在健康成年雌性酉州乌羊（（19.16±1.44）kg）、板角山羊（（23.27±3.24）kg）皮肤组织中的mRNA水平，及其在小鼠皮肤黑色素瘤细胞（B16-F10）增殖、分化过程中（培养1、3、5和7 d）的表达模式，并分析HOTAIR与其他3个基因表达的相关性。结果显示，在皮肤组织中，酉州乌羊HOTAIR和HOXC12的相对表达量极显著高于板角山羊（P<0.01），酉州乌羊HOXD10的相对表达量显著低于板角山羊（P<0.05）；HOTAIR与HOXC12表达显著正相关（P<0.05），与HOXD10表达显著负相关（P<0.05）。在B16-F10细胞增殖阶段，HOTAIR在各阶段表达差异不显著（P>0.05），HOXC11、HOXC12和HOXD10呈"高-低-高"的表达模式，且均在培养1 d的相对表达量极显著高于3和5 d（P<0.01）；HOTAIR与HOXC11表达呈极显著正相关（P<0.01）。在B16-F10细胞分化阶段，HOTAIR表达持续上升，HOXD10表达持续下降，HOXC11在各阶段的相对表达差异不显著（P>0.05），HOXC12在培养1和5 d的相对表达量极显著高于3和7 d（P<0.01）；HOTAIR与HOXD10表达极显著负相关（P<0.01）。HOTAIR在乌皮山羊皮肤中高表达，在B16-F10细胞分化阶段的表达随着培养时间的延长而增加，HOTAIR对HOXD10存在负向调控作用。HOTAIR与HOXD10互作可能调控山羊皮肤黑色素细胞的分化与黑色素生成。     

Cloning RPL11 cDNA 3'' End by SMART RACE Technique

RT-PCR and RACE techniques were used to clone 3' end real sequence of RPL11 gene from a goat. The sequences included some non-structural protein (3D) genes, and 3D gene immediately downstream 3' non-coding region (UTR) and poly (A) tail. The results showed that the length of specific amplified fragment was 566 bp (not including polyA tail). RPL11 gene encoded 188 amino acids, amino acid sequence was conducted for homology analysis by NCBI BLAST tool. The highest homology was Mus (RPL11, mRNA homology 99%), ...     

山羊胃的腹腔镜探查

将腹腔镜从山羊胸骨柄后缘的腹中线插入腹腔,观察山羊在仰卧状态下瘤胃、网胃、瓣胃和皱胃,以及它们与肝脏、膈肌、腹壁和肠管的位置关系,并获得清晰的图片,为诊断和治疗反刍动物胃部疾病提供依据。     

舍饲条件下不同出生年份对中卫山羊羔羊初生重的影响

对舍饲条件下2004--2010年7年间中卫山羊羔羊初生重进行分析研究。结果表明，2004--2010年7年间的羔羊初生重均非常接近，且差异均不显著（P〉0．05），其中，2007年公羔羊初生重依次分别比其他年份羔羊初生重提高12．87％、12．04％、3．37％、10．83％、10．04％和10．83％，而2007年母羔羊初生重依次分别提高8．88％、11．02％、1．43％、5．22％、11．46％和2．55％；相同年份间的初生重公羔均比母羔高，但差异不显著（P〉0．05），其中，2004--2010年公羔初生重分别比母羔高5．02％、7．87％、6．84％、8．87％、3.36％、10．27％和0．70％。     

如何防治仔猪黄白痢——论山区农村零散饲养

由于山区农村部分群众受陈旧观念的影响,不知道如何科学饲养管理母猪、仔猪,导致仔猪黄白痢病症成为制约山区农村养猪业发展的重要因素之一。     

Environmental factors regulating the recruitment of walleye Sander vitreus and white bass Morone chrysops in irrigation reservoirs

Understanding the environmental factors that regulate fish recruitment is essential for effective management of fisheries. Generally, first‐year survival, and therefore recruitment, is inherently less consistent in systems with high intra‐ and interannual variability. Irrigation reservoirs display sporadic patterns of annual drawdown, which can pose a substantial challenge to recruitment of fishes. We developed species‐specific models using an 18‐year data set compiled from state and federal agencies to investigate variables that regulate the recruitment of walleye Sander vitreus and white bass Morone chrysops in irrigation reservoirs in south‐west Nebraska, USA. The candidate model set for walleye included only abiotic variables (water‐level elevation, minimum daily air temperature during winter prior to hatching, annual precipitation, spring warming rate and May reservoir discharge), and the candidate model set for white bass included primarily biotic variables (catch per unit effort (CPUE) of black crappie Pomoxis nigromaculatus, CPUE of age‐0 walleye, CPUE of bluegill Lepomis macrochirus and CPUE of age‐3 and older white bass), each of which had a greater relative importance than the single abiotic variable (minimum daily air temperature during winter after hatching). Our findings improve the understanding of the recruitment of fishes in irrigation reservoirs and the relative roles of abiotic and biotic factors.     

Investigations on the light and scanning electron microscopic structure of the lingual papillae in the angora goat (Capra hircus): II. Mechanical papillae

This study was conducted to examine the light microscopy (LM) and scanning electron microscopic (SEM) structures of mechanical papillae on the tongue in the Angora goat (Capra hircus). As study materials, the tongues of four adult female Angora goats were used. The samples were collected from the dorsal surfaces of the apex, body, root and torus of the tongue and the ventral surfaces of the lingual apex for light and scanning electron microscopic examinations. Three types of mechanical papillae were seen in Angora goat tongue: papilla filiformes, papilla lentiformes and papilla conicae.The filiform papillae were detected in the dorsal surface of the tongue from lingual apex to lingual torus, and in the ventro-lateral of the lingual apex and on both sides of the lingual torus. The morphological differences were observed in filiform papillae according to their location in the tongue. The lenticular papillae settled on the centre of the lingual torus. Two types of these papillae, irregular-round and pyramid-shaped were identified. The conical papillae were scattered all over the lingual torus, except for the central part and were also seen on the root of the tongue. In the light microscopic examination, it was found that mechanical papillae had a stratified squamous epithelium and a varying degree of keratin layer on epithelial surfaces. In this study, by examining the light and scanning electron microscopic structure of the mechanical papillae in the Angora goat tongue, their similarities and differences with other domestic and wild ruminant species were determined.     

IFN-α对山羊副流感病毒3型的抗病毒活性

旨在探讨原核表达纯化的山羊α干扰素(IFN-α)对山羊副流感病毒3型(CPIV3)的抗病毒活性。通过分析山羊IFN-α的序列特点,比对不同种属IFN-α的同源性,进而构建山羊IFN-α成熟蛋白编码基因(去除信号肽基因序列)的原核表达载体pET-30a-gIFN-α,将其转化至感受态细胞Rosetta (DE3),IPTG诱导后镍柱及亲和纯化获得山羊IFN-α。利用RT-qPCR测定山羊IFN-α作用于牛肾细胞(Madin-Darby bovine kidney cell, MDBK cell)后6种干扰素刺激基因(interferon-stimulated genes, ISGs)的相对表达水平;同时,利用TCID₅₀及Western blot测定其对CPIV3的抗病毒活性。结果表明,原核表达的山羊IFN-α蛋白含量为0.20 mg·mL^-1,Western blot结果表明表达产物相对分子质量约为20 ku,与预期结果相符。RT-qPCR结果显示,山羊IFN-α孵育MDBK细胞后,可显著刺激RSAD2、STAT1及ISG15等6种ISGs基...     

花椒天牛空间分布型的研究

１９９２～１９９３年在河北省涉县对花椒天牛在树上的分布规律以及空间分布型进行了系统研究。结果表明：在同一环境中植株越大受害愈重；老熟幼虫有８８．６％分布于１ｍ以下的树干上；花椒天牛幼虫的空间分布型属于聚集分布。还对其理论抽样数进行了研究。     

菊花白锈病菌冬孢子萌发的生物学特性

菊花(Dendranthema × grandiflorum(Ramat) Kitam.)是中国传统名花,其花色丰富,花型各异,深受人们的喜爱.同时菊花也是世界四大切花之一,在国际花卉贸易中占有重要的经济地位.