Regulatory role of l-proline in fetal pig growth and intestinal epithelial cell proliferation

l-proline (Pro) is a precursor of ornithine, which is converted into polyamines via ornithine decarboxylase (ODC). Polyamines plays a key role in the proliferation of intestinal epithelial cells. The study investigated the effect of Pro on polyamine metabolism and cell proliferation on porcine enterocytes in vivo and in vitro. Twenty-four Huanjiang mini-pigs were randomly assigned into 1 of 3 groups and fed a basal diet that contained 0.77% alanine (Ala, iso-nitrogenous control), 1% Pro or 1% Pro + 0.0167% α-difluoromethylornithine (DFMO) from d 15 to 70 of gestation. The fetal body weight and number of fetuses per litter were determined, and the small and large intestines were obtained on d 70 ± 1.78 of gestation. The in vitro study was performed in intestinal porcine epithelial (IPEC-J2) cells cultured in Dulbecco''s modified Eagle medium-high glucose (DMEM-H) containing 0 μmol/L Pro, 400 μmol/L Pro, or 400 μmol/L Pro + 10 mmol/L DFMO for 4 d. The results showed that maternal dietary supplementation with 1% Pro increased fetal weight; the protein and DNA concentrations of the fetal small intestine; and mRNA levels for potassium voltage-gated channel, shaker-related subfamily, member 1 (Kv1.1) in the fetal small and large intestines (P < 0.05). Supplementing Pro to either gilts or IPEC-J2 cells increased ODC protein abundances and polyamine concentrations in the fetal intestines and IPEC-J2 cells (P < 0.05). In comparison with the Pro group, the combined administration of Pro and DFMO reduced the expression of ODC protein and spermine concentration in the fetal intestine, as well as the concentrations of putrescine, spermidine and spermine in IPEC-J2 cells (P < 0.05). Meanwhile, the percentage of cells in the S-phase and the mRNA levels of proto-oncogenes c-fos and c-myc were increased in response to Pro supplementation, whereas depletion of cellular polyamines with DFMO increased tumor protein p53 (p53) mRNA levels (P < 0.05). Taken together, dietary supplementation with Pro improved fetal pig growth and intestinal epithelial cell proliferation via enhancing polyamine synthesis.     

生物炭对酸化茶园土壤性状和真菌群落结构的影响

为探讨生物炭长期施用对酸化茶园土壤改良和真菌群落结构的影响,分析了按生物炭用量0、2.5、5、10、20、40 t·hm^-2施用5年后的茶园土壤性状和真菌群落结构变化。结果表明,施用生物炭5年后的茶园土壤pH提高了0.16~1.11,可溶性有机碳含量提高了52.6%~92.3%,而铵态氮和硝态氮含量以10 t·hm^-2处理最高。施用生物炭5年后的土壤性质变化,进一步影响了真菌群落结构,表现为Chao指数、ACE指数和Shannon指数随生物炭用量增加呈先增加后降低的趋势;提高生物炭施用量对茶园土壤次要作用的真菌（LDA值<3.50）丰度的增加效果高于优势真菌（LDA值>3.50）的效果,其中被孢霉属、木霉属、毛壳菌属的相对丰度增加,黑盘孢属的相对丰度降低。     

Soil quality assessments in integrated crop–livestock–forest systems: A review

Integrated crop–livestock–forest is a promising strategy to improve soil quality. It comprises four different integrated farming systems: crop–livestock, crop–forest, forest–livestock and crop–livestock–forest. This work systematically reviewed studies about integrated crop–livestock–forest systems and soil quality. A total of 92 papers were retrieved from the Web of Science—Clarivate Analytics platform, and the following information was analysed: publication year, institution, region of the studied site, type of integrated system, soil type, tillage system, maximum soil depth and the soil quality indicators assessed. Most studies were published in the second half of the 2010s. Brazil is a prominent focus of research about soil quality and integrated crop–livestock–forest systems, with significant contribution from its central and southern regions. The Embrapa was the main publishing institution, present in over one‐third of the studies. Crop–livestock was the most common integrated system, Ferralsols was the most common soil group, and most of the studied soils were clayey. No tillage was the main tillage system. Most studies focused on the topsoil, assessing physical and/or chemical soil quality indicators. More emphasis on biological indicators of soil quality is required, as well as assessments integrating biological, physical and chemical indicators of soil quality. Future works should compare different integrated systems, including assessments deeper in the soil profile, especially in systems with the forest component, and also in sandy and silty soils. Soil quality indicators that have been rarely used should be further tested. Novel indicators should be added to better understand the promotion of soil quality by integrated crop–livestock–forest systems.     

有机肥对土壤特性及农产品产量和品质影响研究进展

施用有机肥是农业生产的重要措施,对绿色农业发展起到积极作用,而且越来越广泛施用。本文介绍了目前有机肥基本概况,重点综述了有机肥对提高土壤肥力、提高作物产量和品质、促进养分利用以及对病虫害发生影响等方面研究的最新进展,分析了当前有机肥施用对耕地质量和农产品安全风险、施肥效率低、以及有机肥品种研发不足、相关质量标准滞后等问题,并对当前有机肥推广应用的形势和发展前景进行了分析和展望。     

Nutrients in litterfall,forest floor and mineral soils in two adjacent forest ecosystems in Greece

The fluxes of masses and the nutrients Ca,Mg,K,N,P and S were determined in the litterfall of two adjacent forest ecosystems of Hungarian oak(Quercus frainetto L.)and European beech(Fagus sylvatica L.)in a mountainous area of northeastern Greece in 2010–2015.The foliar litterfall for both species reached about 70%of the total litterfall,and was significantly higher from the other two fractions(woody and rest litterfall).The fluxes of masses and nutrients were compared between ecosystems for each fraction separately.Only one significant statistical difference was found,that of K in the woody litterfall.In addition,the stocks of masses and nutrients were calculated in the forest floors and mineral soils of the two ecosystems.Likewise,the stocks of nutrients in the forest floors and mineral soils were compared between ecosystems.In the L horizon of the forest floors,statistical differences,as a result of species effect,were found for the stocks of Ca and N.In the FH horizons,the masses and all the nutrient stocks differed significantly,as the beech plot had much higher quantities of organic matter and nutrients.These higher quantities were probably due to low soil temperatures(microclimate)and high acidity in the beech plot(species effect)that slowed down decomposition.In the mineral soils,the propagation of random error derived from random errors of the individual soil layers was an important factor in the statistical comparisons.Because of the soil acidity in the beech plot,the stocks of exchangeable base cations were significantly higher in the oak plot,whereas the other nutrient stocks did not differ.     

热风干燥过程中山药水分状态的变化研究

为了解山药的水分赋存状态的变化，以襄阳道地山药为材料，分别在60、70、80、90、100 ℃条件下进行热风干燥，采用低场核磁共振（LF-NMR）和差式量热扫描（DSC）技术，每隔15 min测定山药在热风干燥过程中的水分状态及迁移规律。结果表明，在60~90 ℃条件下，温度越高，干基含水率的降速越快。山药的T2弛豫图谱有3个较为明显的吸收峰，随干燥进程的延续，各峰面积均明显减少，其中自由水所在峰的面积降幅最大，表明干燥过程中自由水散失最多，而且自由水逐渐向半结合水和结合水迁移，冷冻峰和解冻峰也随之变小。但在100 ℃下干燥时，样品可能因表面板结导致干基含水率、低场核磁吸收峰升高，冷冻和解冻峰面积增加。因此，在实际干燥过程中，山药的热风干燥温度不宜高至100 ℃。     

Identification of an H1N1 subtype of swine influenza virus and serological analysis

To investigate the epizootic of swine influenza virus(SIV), 60 nasal swabs were collected from a clinical cases of pig farm in Tai'an City, Shandong Province of China in April 2017. SIV was isolated by inoculating into 10-day-old Special Pathogen Free embryonated eggs and the whole genome was sequenced. An H1N1 subtype SIV was isolated and designated as A/swine/Shandong/TA04/2017(H1N1). Phylogenetic analysis showed that apart from the polymerase A(PA) fragment belonging to the 2009 pandemic H1N1 branch, seven genome segments belonged to avian-like H1N1 influenza virus lineage. The cleavage site sequence of the hemagglutinin(HA) protein was PSIQSR↓G, which is a typical molecular biological characteristic. Five potential N-glycosylation sites(N14, N26, N277, N484 and N543) were found in the HA gene. To further investigate the epidemiology of SIV in this farm, the 995 serum samples were assessed with EAH1N1 2009 pandemic H1N1 and H3 N2 antigens. The results showed that the total positive rate was 65.43%. The positive rates of single virus infection detected by EAH1N1, 2009 pdmH1N1 and H3 N2 for serum HI(Hemagglutination inhibition) were 48.35, 30.85 and 7.47%, respectively. The results showed that SIV in Shandong Province has been reassorted in some segments and the SIV-positive rate was high on the SIV outbreak farm. These data provide evidence of an epizootic of SIV.     

面向养殖水体分布差异的COD光谱法检测研究

面向养殖水体，传统光谱法对化学需氧量（Chemical Oxygen Demand，COD）检测模型构建的基础：源域（现有样本库）与目标域（检测地水体）间光谱数据独立同分布。但是当源域与目标域分布间存在差异时，由源域得到的低误差模型常在目标域上表现下滑。针对该问题，提出面向UV Vis光谱的域对抗训练网络（DAUVwpNet），将分布不同的源域和目标域数据映射至相同分布的特征空间中，使其在该空间的分布距离尽可能接近，从而在特征空间中对源域训练的目标函数也可以迁移至目标域上，以降低模型在目标域的误差。试验表明：面向同一批测试数据，DAUVwpNet的预测误差为0.78，要低于传统模型的预测误差（0.85）；DAUVwpNet预测值与实测值间相关系数为0.95，要高于传统模型的相关系数（0.89）。表明了该网络能够较好对齐两域特征空间数据分布，降低因分布差异带来的COD检测误差。