试论我国科技期刊发展的集团化趋势

在分析我国科技期刊发展的现状的基础上,提出了集团化是科技期刊发展的必然趋势。     

中草药制剂对泌乳奶牛机体免疫性能的影响

试验采用大青叶、大黄、党参等19味中草药分别组成内服和子宫灌注制剂治疗奶牛子宫内膜炎，并采集颈静脉血测定淋巴细胞刺激指数（SI）和中性粒细胞吞噬力，以观察中草药制剂对奶牛免疫性能的影响。结果显示，中草药制剂均可提高奶牛免疫力，内服中草药制剂应用效果优于子宫灌注制剂（P〈0．05）；复方制剂优于单味中草药制剂（P〈0．05）；复方中草药内服制剂可使淋巴细胞刺激指数（SI）和中性粒细胞吞噬力分别提高53．76％和4．58％（P〈0．01）；复方子宫灌注制剂可使淋巴细胞刺激指数（SI）和中性粒细胞吞噬力分别提高39．58％和1．98％（P〈0．01）。     

Screening and Analysis of Proteins Interacting with TaPDK from Physiological Male Sterility Induced by CHA in Wheat

To further research the regulatory network of pyruvate dehydrogenase kinase (designated as TaPDK) in physiological male-sterility (PHYMS) of wheat induced by chemical hybridizing agent (CHA) SQ-1, an anther cDNA library was constructed, and the proteins interacting with TaPDK were screened via yeast two-hybrid technique. Subsequently, a few candidate proteins in nucleotide expression levels were detected by real-time quantitative PCR. Yeast-two hybrid screening was performed by mating yeast strain Y2HGold containing BD-TaPDK bait plasmid with yeast strain Y187 including anther cDNA library plasmid. Diploid yeast cells were plated on synthetic dropout nutrient medium (SD/-Ade/-His/-Leu/-Trp) (QDO), and further were incubated on QDO medium containing AbA and X-α-Gal. The interactions between TaPDK and the proteins obtained from positive colonies were further confirmed by co-transformation validation. After plasmids DNA were extracted from blue colonies and sequenced, the sequences results were analyzed by bioinformatic methods. Finally, 24 colonies were obtained, including eight genes, namely non-specific lipid-transfer protein precursor (TanLTP), polyubiquitin (TaPUbi), glyceraldehyde-3-phosphate dehydrogenase, proliferating cell nuclear antigen (TaPCNA), CBS domain containing protein (TaCBS), actin, guanine nucleotide-binding protein beta subunit, chalcone synthase, and three new genes with unknown function. The results of quantitative RT-PCR showed that the expression levels of TanLTP, TaPUbi, and TaPCNA were obviously up-regulated in PHYMS anther, and TaCBS expression was only increased at the tricellular stage in PHYMS anther compared with in fertile lines. Whereas, the expression of TaPDK was obviously down-regulated in PHYMS lines. Collectively, these datas indicated that the majority of candidate proteins might be related to pollen abortion in PHYMS lines, which further suggested that TaPDK plays multiple roles in pollen development, besides participating in regulating pyruvate dehydrogenase complex activity.     

广西农产品物流企业WLC品牌元素的选择研究

针对广西农产品物流企业的特点,从众多影响企业品牌建设与发展的因素中,采用AHP-TOPSIS模型选择确定品牌全生命周期阶段对品牌竞争力影响力度最大的品牌元素,以实现品牌的可持续发展。     

4种不同杀虫剂防治淮山斜纹夜蛾药效试验

通过选用4种不同的杀虫剂防治淮山斜纹夜蛾试验，结果表明：药后10d，10亿PIB／mL银纹夜蛾多角体病毒悬浮剂（奥绿一号）处理的防效达100％，0．15％甲氨阿维·1000万PIB／mL苜银夜核悬浮剂（攻蛾）防效达92．8％，15％杜邦安打悬浮剂防效达91．7％，这3种药剂值得在生产上推广应用。     

Effects of Shading on Chlorophyll Content,Chlorophyll Fluorescence and Photosynthesis of Subterranean Clover

A field‐experiment (2004/2005 and 2005/2006 seasons) was conducted in the coastal plain of south‐eastern Sicily (37°03′N, 15°18′E, 15 m a.s.l.), on a Calcixerollic Xerochrepts soil, aimed at quantifying the effect of shading on chlorophyll (Chl) content, Chl fluorescence, photosynthesis and growth of subterranean clover. Four levels of photosynthetically active radiation reduction (from 0 % to 90 %) were tested on Trifolium brachycalycinum cv. ‘Clare’ and Trifolium subterraneum ecotype ‘Ragalna’. In both species shading progressively increased F_v/F_m, internal CO₂ concentration, diffusive leaf resistance and specific leaf area (up to 8 %, 34 %, 18 % and 68 %, respectively), and decreased Chl content, T_max, photosynthetic rate and plant dry weight (up to 9 %, 24 %, 79 % and 39 %, respectively). As plants aged, characteristic bell‐shaped trends were evident for photosynthetic parameters, with F_v/F_m increasing up until the onset of flowering, and thereafter declining. This implies that F_v/F_m may be a useful indicator of earliness in subterranean clover genotypes. The aboveground dry biomass response to shading was both genotype‐ and season‐dependent, but was predictable from the measurement of relative leaf Chl content. Moreover, our results suggest that an improvement in the interaction between host‐rhizobium may represent a major potential breeding target for enhancing subterranean clover tolerance to shading.     

金针菇航天种菌丝诱变效应的研究

本实验以2个金针菇航天诱变菌种(航金1号和航金2号)和地面对照菌种(江山白)为材料,进行了拮抗实验、菌丝生长速度实验、抗杂性实验和酯酶同工酶分析实验研究,结果表明,金针菇航天种与对照之间有拮抗现象,但无拮抗线。航天搭载菌株的菌丝生长速率比对照快,其中航金1号生长速度最快。航金1号和航金2号均表现出较强的抗杂性,能抑制并盖过霉菌菌丝生长。通过酯酶同工酶聚丙烯酰胺凝胶电泳分析发现,航金1号和航金2号与对照相比都出现了特异谱带,说明经过航天诱变的菌株发生了一定的突变。     

不同肥料处理对盆栽红豆杉生长的影响

研究了不同肥料处理对盆栽红豆杉的茎高、新枝、根长和鲜重的影响。结果表明:每周对红豆杉叶面喷施3‰磷酸二氢钾,对红豆杉的生长效果最好。     

W/O微乳液体系中SA和MS清除·O_2~-的研究

以水溶液体系作为对照,利用分光光度法研究了W/O型微乳液体系中水杨酸（SA）和水杨酸甲酯（MS）清除超氧阴离子自由基（·O2-）的性能.结果发现,在水浴时间为20min、pH值为8.20和邻苯三酚加入量适宜的情况下,W/O微乳液体系中SA、MS对·O2-的清除率分别可达72.92%、81.47%.微小的pH值变化会引起邻苯三酚自氧化速率的显著变化,电解质溶液对其也有明显的影响,但对SA、MS清除·O2-的能力影响不大.     

含LacZ重组逆转录病毒的制备及其在NIH3T3细胞的表达

构建了含有3．7kbLacZ的重组逆转录病毒载体,转染包装细胞系PA317后,经G418筛选,得到了G418的抗性的产毒细胞系,用收获的含有重组逆转录病毒粒子的浓缩病毒上清,感染NIH3T3细胞。经X-gal染色检测,发现表达了LacZ的NIH3T3细胞呈蓝色。