阿维菌素·灭幼脲Ⅲ号防治马尾松毛虫技术

阿维菌素·灭幼脲Ⅲ号为阿维菌素和灭幼脲Ⅲ号两种生物农药的复合制剂,有激素和抗生素两类杀虫剂的特点,具胃毒兼触杀之功效.运用阿维菌素.灭幼脲Ⅲ号进行低剂量喷雾防治马尾松毛虫具有良好的防治效果;在使用阿维菌素·灭幼脲Ⅲ号防治马尾松毛虫时,应采用80 mL/hm2(600倍液)的剂量进行低剂量均匀喷雾,可达到药物用量少、防效...     

偶氮氯磷Ⅲ吸光光度法测定工业碳酸锂中微量钙

研究了工业碳酸锂中微量钙的测定方法。采用偶氮氯磷Ⅲ［２，７－双－（４－氯─２－膦苯偶氮）－１，８－二羟基苯酚－３，６－二磺酸］作显色剂，在ｐＨ＝２．３─２．７条件下，钙离子与偶氮氯磷Ⅲ生成水溶性蓝色配合物，最适测定波长为λ＝６５８ｎｍ，摩尔吸光系数ε＝３．１×１０４，有色溶液稳定６小时，钙含量在０─１μｇ／ｍＬ范围内服从比耳定律。该显色剂用于工业碳酸锂中微量钙的测定与原子吸收法相比较，结果一致。     

基于物种敏感性分布（Burr-Ⅲ）模型预测Cd对水稻毒害的生态风险阈值HC5

Burr-Ⅲ型分布是一种灵活的分布函数模型,对有害物质产生毒性的物种敏感性数据有很好的拟合效果。本研究采用水培实验研究了我国常见的17种不同水稻对Cd毒性的剂量-效应关系,结合Burr—Ⅲ物种敏感性分布模型对不同水稻Cd毒性的物种敏感性分布频次和基于保护95％水稻品种的Cd毒性阈值HC5进行了预测。结果表明：水培条件下,随着Cd处理浓度（0．30 - 6．0mg·L^-1）的增加,水稻对Cd富集系数（SCV）明显下降,而转运系数（TF）呈现出增加的趋势,总体而言,杂交品种水稻对Cd的转运系数高于常规品种水稻。不同水稻对Cd胁迫的半抑制浓度阈值（EC50）变化范围为0．552—24．01mg·L^-1,不同水稻品种EC50相差1．18-43．49倍,10％抑制浓度（EC10）变化范围为0．033～1．624mg·L^-1,不同水稻品种EC50相差1．758-49．21倍。不同水稻对Cd的毒性呈现出明显的敏感性差异特征,Bur-Ⅲ分布模型预测结果表明,基于保护95％的水稻品种,Cd的10％抑制浓度值（HC5^10%）为0．045mg·L^-1,50％抑制浓度值（HC550％）为0．594mg·L^-1。研究结果为我国水稻Cd污染防治及土壤Cd质量标准的修订提供了依据。     

蚓激酶F-Ⅲ-1基因植物表达载体的构建

[目的]探讨蚓激酶F-III-1基因在烟草中的高效表达。[方法]以经烟草密码子优化、人工合成的蚓激酶成熟肽F-III-1为目的基因,以p CAMBIA3301和p BA002质粒为基础,构建由35S启动子调控、抗草铵膦基因(bar)为选择标记的植物表达载体。[结果]成功构建了4个植物表达载体,分别是p CAMBIA3301-F-III-1、p CAMBIA3301-F-III-1-6x His、p BA002-F-III-1和p BA002-F-III-1-6x His。[结论]4个植物表达载体的成功构建为探讨蚓激酶在植物中的表达及其植物反应器生产奠定了基础。     

海水常见革兰氏阴性病原菌Ⅲ型分泌系统的研究进展

许多革兰氏阴性病原细菌借助细菌的Ⅲ型分泌系统(Type Ⅲ secretion system,T3SS)传递与致病有关的毒性因子和效应子来发挥病原细菌的毒性.副溶血弧菌(Vibrio parahaemolyticus)、迟缓爱德华民菌(Edwardsiella tarda)和嗜水气单胞菌(Aeromonas hydrophila)是海水中3种常见革兰氏阴性病原细菌.这3种细菌不仅广泛引起海水养殖生物的病害,还经常导致人类肠胃炎等其他危害,而Ⅲ型分泌系统也与它们的致病性密切相关.对海水中这3种常见革兰氏阴性病原菌Ⅲ型分泌系统的组成、功能及相关转录调控机制进行了综述.     

Rescue of Genotype Ⅲ Velogenic Newcastle Disease Virus Strain JS/7/05/Ch

The genome of JS/7/05/Ch isolate shared more than 99% nucleotide identity with that of Mukteswar strain. However, the pathogenicity of JS/7/05/Ch was much stronger than that of Mukteswar strain. In order to provide a good foundation for the further related research, we built the rescue system of JS/7/05/Ch in this study. Based on the genomic sequence of JS/7/05/Ch strain, eight pairs of primers were designed to amplify the genomic fragments by RT-PCR and cloned into pCR2.1 vector, to construct the plasmid pJS/7/05/Ch which contained the full-length cDNA of NDV. Then the full-length cDNA was transferred to TVT7R(0.0) vector to construct full-length NDV infectious clone, pTVT/JS705, using specific enzymes. The infectious clone together with three helper plasmids (pCI-NP, pCI-P and pCI-L) were cotransfected into BSR-T7/5 cell, and the transfection supernatant was inoculated into SPF embryonated eggs to rescue the virus. The results of HA and RT-PCR indicated that JS/7/05/Ch strain of NDV was successfully rescued.     

Knowledge Mapping Analysis on Global Bovine Mastitis Vaccine Research

To understand the overall status and focus of bovine mastitis vaccine research,knowledge maps of mainly country,institution,author,cite journal,cite reference,keyword,year distribution of publications on research of bovine mastitis vaccine were studied by Citespace Ⅲ,which collected 4 205 international scientific literatures download from Web of Science^TM core collection (1995 to 2015).The results showed that in research field of bovine mastitis vaccine,the number of published papers increased from 1995 to 2012,but it began to decrease in 2013;Countries and institution involved in the research were mainly distributed in America,Europe and Asia,and universities played important roles in this field;In addition,the research field were mainly focus on:Isolation and identification of pathogens,detection of drug susceptibility and pathogenesis exploration;Immune response and relevant regulatory factor in infected dairy cows;Best period for prevention and treatment;Screening for candidate antigen.In future time,studies will pay more attention on research of NF-κB and biofilm in field of bovine mastitis vaccine.     

多糖对水稻土中异化Fe(Ⅲ)还原过程的影响

本研究通过土壤泥浆厌氧培养的方法,在水稻土中添加不同质量浓度淀粉和纤维素,探讨多糖作为电子供体对异化Fe(Ⅲ)还原过程的影响。结果表明,在2种水稻土泥浆的厌氧培养过程中,淀粉、纤维素可以促进水稻土中的异化Fe(Ⅲ)还原,其质量浓度在0～20 g/L时,Fe(Ⅱ)最大累积量和速率常数随质量浓度增加而增大,其对土壤中Fe(Ⅲ)氧化物异化还原的促进作用与土壤pH、有机质和无定形铁含量有关。对培养过程中pH与异化铁还原动力学数据的比较发现,在微生物正常生长的pH范围内,较低的pH利于Fe(Ⅲ)的还原。     

甘蔗蔗糖磷酸合成酶磷酸化位点的突变及相应表达载体的构建

用Clontech公司的定点突变试剂盒TransformerTMSite-Directed Mutagenesis Kit对克隆载体pMD18T-SPS3L上的SPS基因SPS3L进行定点突变,使其459位的丝氨酸密码子分别突变为编码苏氨酸、丙氨酸和谷氨酸的密码子。然后将突变后的和未突变的SPS3L基因分别克隆至表达载体质粒pCAMBIA1301中,构建重组质粒pCAMBIA1301-SPS3L。经测序鉴定,对SPS3L基因的定点突变成功,构建表达载体pCAMBIA1301-SPS3L成功。     

植物膜脂过氧化作用和内源激素对UV-B辐射和稀土的响应

[目的]为稀土农用拓展及环境污染防治提供策略。[方法]采用水培法研究了大豆幼苗（Glycinemax）膜脂过氧化作用和内源激素对UV-B辐射和稀土的响应。[结果]UV-B（T1-0.15W/m2和T2-0.45W/m2）辐射下,大豆幼苗叶绿素（Chl）和吲哚乙酸（IAA）含量在胁迫期（1～5d）逐渐下降,在恢复期（6～11d）上升;丙二醛（MDA）和脱落酸（ABA）含量先增（1～5d）后减（6～11d）。20mg/LLa（Ⅲ）减缓了上述指标在胁迫期的下降/上升趋势,加快了恢复期的上升/下降速度。[结论]La（Ⅲ）对大豆幼苗膜脂过氧化作用和内源激素的调控能提高叶绿素和IAA含量,改善活性氧的新陈代谢,抑制膜脂过氧化作用,减少ABA积累,且对低剂量紫外辐射（T1）的防护效果优于高剂量紫外辐射（T2）。 更多还原