| 甘蔗蔗糖磷酸合成酶磷酸化位点的突变及相应表达载体的构建 |
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| 引用本文： | 胡巍,张积森,叶冰莹,陈由强,陈如凯.甘蔗蔗糖磷酸合成酶磷酸化位点的突变及相应表达载体的构建[J].甘蔗(福建),2010(4):280-283. |
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| 作者姓名： | 胡巍 张积森 叶冰莹 陈由强 陈如凯 |
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| 作者单位： | [1]福建师范大学生命科学学院,福建福州350108 [2]农业部甘蔗遗传改良重点开放实验室,福建福州350108 |
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| 基金项目： | 国家自然科学基金项目（30871575）; 国家高技术研究发展计划“863”项目（SQ2007AA10XK140067） |
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| 摘 要： | 用Clontech公司的定点突变试剂盒TransformerTMSite-Directed Mutagenesis Kit对克隆载体pMD18T-SPS3L上的SPS基因SPS3L进行定点突变,使其459位的丝氨酸密码子分别突变为编码苏氨酸、丙氨酸和谷氨酸的密码子。然后将突变后的和未突变的SPS3L基因分别克隆至表达载体质粒pCAMBIA1301中,构建重组质粒pCAMBIA1301-SPS3L。经测序鉴定,对SPS3L基因的定点突变成功,构建表达载体pCAMBIA1301-SPS3L成功。
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| 关 键 词： | 甘蔗 SPSⅢ 磷酸化位点 定点突变 表达载体 |
Site-directed mutagensis of the phosphorylation site of sucrose phosphate synthase from Saccharum officinarum and constructed the relation expression vector |
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| HE Wei,ZHANG Ji-sen,YE Bing-ying,CHEN You-qiang,CHEN Ru-kai.Site-directed mutagensis of the phosphorylation site of sucrose phosphate synthase from Saccharum officinarum and constructed the relation expression vector[J].Sugarcane,2010(4):280-283. |
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| Authors: | HE Wei ZHANG Ji-sen YE Bing-ying CHEN You-qiang CHEN Ru-kai |
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| Institution: | 1.College of Life Sciences,Fujian Normal University,Fuzhou,Fujian 350108,China;2.Key Laboratory of Genetics Improvement for Sugarcane,Ministry of Agriculture,Fuzhou,Fujian 350108,China) |
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| Abstract: | The SPS3L gene of sucrose phosphate synthase（SPS） on the vector pMD18T-SPS3L was Site-directed Mutagensised by the TransformerTM Site-Directed Mutagenesis Kit（Clontech）,the Ser condon which at 456 to 459 was respectively mutagensised into three condons（Thr,Ala,Glu）.The mutagensised and unmutagensised SPS3L were cloned into expression vector pCAMBIA1301-SPS3L.According to the sequenced result,the Site-directed Mutagensis was correct and the expression vector pCAMBIA1301-sps3L was constructed successfully. |
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| Keywords: | Saccharum officinarum sucrose phosphate synthase Ⅲ phosphorylation site site-directed mutagensis expression vector |
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